Fetal bovine serum adsorption onto hydroxyapatite sensor monitoring by quartz crystal microbalance with dissipation technique

Motohiro Tagaya, Toshiyuki Ikoma, Satoshi Migita, Mitsuhiro Okuda, Taro Takemura, Nobutaka Hanagata, Tomohiko Yoshioka, Junzo Tanaka

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Adsorption behavior of fetal bovine serum (FBS) onto hydroxyapatite (HAp) was analyzed in situ in phosphate buffer saline (PBS), minimum essential mediums (αMEM or DMEM), and different carbonate-concentrated buffers by using a quartz crystal microbalance with dissipation (QCM-D) technique. Although the adsorption behaviors of FBS onto gold showed no dependence on the solvents, those onto HAp clearly depended on the adsorbed ions in the solvents (PBS or MEMs). The amount and viscoelasticity of FBS adsorbed onto HAp in PBS were approximately two times larger than those in MEMs. The adsorption amounts of carbonate ions on HAp in the carbonate buffers increased with increasing carbonate concentrations, which inhibited the adsorption of FBS and decreased the viscoelastic property of the adsorption layers.

Original languageEnglish
Pages (from-to)176-181
Number of pages6
JournalMaterials Science and Engineering B: Solid-State Materials for Advanced Technology
Volume173
Issue number1-3
DOIs
Publication statusPublished - Feb 26 2010
Externally publishedYes

Keywords

  • Adsorption
  • Ceramics
  • Ions
  • Surface and interface states
  • Surface structure

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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