Fertilization and blastoderm development of quail oocytes after intracytoplasmic injection of chicken sperm bearing the W chromosome

S. Takagi, T. Ono, A. Tsukada, Y. Atsumi, S. Mizushima, N. Saito, K. Shimada

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Our previous study demonstrated that elongated spermatids and sperm carrying the female-specific W-chromosome of the sex-reversed domestic fowl can activate the mouse oocyte, but whether they can fertilize the avian oocyte and lead to a developing zygote remains undetermined. A single sperm isolated from the semen and testis of normal rooster and from a testis of sex-reversed hen was microinjected into a quail oocyte and cultured for 20 to 24 h. Blastoderms were fixed, cleaved, nuclei stained by 4′,6′-diamidino-2-phenylindole, and developmental stages were assessed. In the normal rooster group, ejaculated and testicular sperm induced blastodermal development in 22.6 and 20% of the quail oocytes, respectively. The developmental stages ranged from IV to VII. In the sex-reversal group, 20% of injected testicular sperm induced blastodermal development. The blastodermal stages varied from stage III to VI. Blastoderms after 4′,6′-diamidino-2-phenylindole staining were assayed by PCR to identify the W chromosome of either chicken sperm or quail oocyte. The PCR assay results showed that 2 out of 9 developed blastoderms microinjected with sperm of sex-reversed hen were identified containing the female-specific W chromosome derived from sex-reversed hen. From these results, it is concluded that chicken sperm bearing the W chromosome possess fertilizing ability and can function to stimulate blastoderm development similar to that of normal chicken sperm carrying the Z chromosome.

Original languageEnglish
Pages (from-to)937-943
Number of pages7
JournalPoultry Science
Volume86
Issue number5
DOIs
Publication statusPublished - May 1 2007

Keywords

  • Blastodermal development
  • Fertilization
  • Sex reversal
  • Sperm
  • W chromosome

ASJC Scopus subject areas

  • Animal Science and Zoology

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