Ferrous iron production mediated by tetrathionate hydrolase in tetrathionate-, sulfur-, and iron-grown Acidithiobacillus ferrooxidans ATCC 23270 cells

Tsuyoshi Sugio, Taher M. Taha, Fumiaki Takeuchi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

When tetrathionate-grown Acidithiobacillus ferrooxidans ATCC 23270 cells were incubated with ferric ions and tetrathionate at pH 3.0, ferrous ions were produced enzymatically. Fe3+-reductase, which catalyzes Fe 3+ reduction with tetrathionate, was purified to homogeneity not only from tetrathionate-grown, but also from sulfur-and iron-grown A. ferrooxidans ATCC 23270 cells. The results for apparent molecular weight measured by SDS-PAGE (52.3 kD) and the N-terminal amino acid sequences of the purified enzymes from iron-, sulfur, and tetrathionate-grown cells (AVAVPMDSTG) indicate that Fe 3+-reductase corresponds to tetrathionate hydrolase. The evidence that tetrathionate-grown A. ferrooxidans ATCC 23270 cells have high ironoxidizing activity at the early log phase, comparable to that of iron-grown ATCC 23270 cells, is supported by our finding that tetrathionate hydrolase produces Fe2+ from tetrathionate during growth on tetrathionate. This is the first report on ferric reductase activity associated with tetrathionate hydrolase.

Original languageEnglish
Pages (from-to)1381-1386
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number6
DOIs
Publication statusPublished - 2009

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Keywords

  • Acidithiobacillus ferrooxidans
  • Fe -reduction
  • Ferrous iron production
  • Tetrathionate hydrolase

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

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