Female mating receptivity inhibited by injection of male-derived extracts in Callosobruchus chinensis

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34 Citations (Scopus)

Abstract

The effects of male-derived extracts on female receptivity to remating were investigated in Callosobruchus chinensis (Coleoptera: Bruchidae). Injection of aqueous extracts of male reproductive tracts into the abdomen of females reduced receptivity. When aqueous extracts of male reproductive tracts were divided to three molecular weight (MW) fractions by ultrafiltration: 14 kDa, the filtrate containing MW substances 14 kDa inhibited receptivity 2 and 4 days after injection. Finally, male reproductive tract organs were divided into accessory gland, seminal vesicle, and testis. Aqueous extracts of testis reduced receptivity of females on the second day and at 3 h, and aqueous extracts of accessory gland reduced receptivity of females on the second day after injection. On the other hand, aqueous extracts of seminal vesicle did not reduce female receptivity. The results indicate that more than one mechanism may be involved in producing the effects of male-derived substances on female receptivity; low MW male-derived substances, which possibly exist in testis, cause short-term inhibition, while high MW substances, which possibly exist in the accessory gland, inhibit female mating later than low MW substances in C. chinensis.

Original languageEnglish
Pages (from-to)501-507
Number of pages7
JournalJournal of Insect Physiology
Volume54
Issue number2
DOIs
Publication statusPublished - Feb 2008

Fingerprint

Callosobruchus chinensis
injection
Injections
extracts
Molecular Weight
molecular weight
Testis
testes
seminal vesicles
Seminal Vesicles
water
Bruchidae
Beetles
filtrates
Ultrafiltration
ultrafiltration
abdomen
Abdomen
Coleoptera

Keywords

  • Accessory gland
  • Adzuki bean beetle
  • Remating inhibition
  • Seminal fluid
  • Testis

ASJC Scopus subject areas

  • Insect Science
  • Physiology

Cite this

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title = "Female mating receptivity inhibited by injection of male-derived extracts in Callosobruchus chinensis",
abstract = "The effects of male-derived extracts on female receptivity to remating were investigated in Callosobruchus chinensis (Coleoptera: Bruchidae). Injection of aqueous extracts of male reproductive tracts into the abdomen of females reduced receptivity. When aqueous extracts of male reproductive tracts were divided to three molecular weight (MW) fractions by ultrafiltration: 14 kDa, the filtrate containing MW substances 14 kDa inhibited receptivity 2 and 4 days after injection. Finally, male reproductive tract organs were divided into accessory gland, seminal vesicle, and testis. Aqueous extracts of testis reduced receptivity of females on the second day and at 3 h, and aqueous extracts of accessory gland reduced receptivity of females on the second day after injection. On the other hand, aqueous extracts of seminal vesicle did not reduce female receptivity. The results indicate that more than one mechanism may be involved in producing the effects of male-derived substances on female receptivity; low MW male-derived substances, which possibly exist in testis, cause short-term inhibition, while high MW substances, which possibly exist in the accessory gland, inhibit female mating later than low MW substances in C. chinensis.",
keywords = "Accessory gland, Adzuki bean beetle, Remating inhibition, Seminal fluid, Testis",
author = "Takashi Yamane and Yoshinobu Kimura and Maki Katsuhara and Takahisa Miyatake",
year = "2008",
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doi = "10.1016/j.jinsphys.2007.11.009",
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T1 - Female mating receptivity inhibited by injection of male-derived extracts in Callosobruchus chinensis

AU - Yamane, Takashi

AU - Kimura, Yoshinobu

AU - Katsuhara, Maki

AU - Miyatake, Takahisa

PY - 2008/2

Y1 - 2008/2

N2 - The effects of male-derived extracts on female receptivity to remating were investigated in Callosobruchus chinensis (Coleoptera: Bruchidae). Injection of aqueous extracts of male reproductive tracts into the abdomen of females reduced receptivity. When aqueous extracts of male reproductive tracts were divided to three molecular weight (MW) fractions by ultrafiltration: 14 kDa, the filtrate containing MW substances 14 kDa inhibited receptivity 2 and 4 days after injection. Finally, male reproductive tract organs were divided into accessory gland, seminal vesicle, and testis. Aqueous extracts of testis reduced receptivity of females on the second day and at 3 h, and aqueous extracts of accessory gland reduced receptivity of females on the second day after injection. On the other hand, aqueous extracts of seminal vesicle did not reduce female receptivity. The results indicate that more than one mechanism may be involved in producing the effects of male-derived substances on female receptivity; low MW male-derived substances, which possibly exist in testis, cause short-term inhibition, while high MW substances, which possibly exist in the accessory gland, inhibit female mating later than low MW substances in C. chinensis.

AB - The effects of male-derived extracts on female receptivity to remating were investigated in Callosobruchus chinensis (Coleoptera: Bruchidae). Injection of aqueous extracts of male reproductive tracts into the abdomen of females reduced receptivity. When aqueous extracts of male reproductive tracts were divided to three molecular weight (MW) fractions by ultrafiltration: 14 kDa, the filtrate containing MW substances 14 kDa inhibited receptivity 2 and 4 days after injection. Finally, male reproductive tract organs were divided into accessory gland, seminal vesicle, and testis. Aqueous extracts of testis reduced receptivity of females on the second day and at 3 h, and aqueous extracts of accessory gland reduced receptivity of females on the second day after injection. On the other hand, aqueous extracts of seminal vesicle did not reduce female receptivity. The results indicate that more than one mechanism may be involved in producing the effects of male-derived substances on female receptivity; low MW male-derived substances, which possibly exist in testis, cause short-term inhibition, while high MW substances, which possibly exist in the accessory gland, inhibit female mating later than low MW substances in C. chinensis.

KW - Accessory gland

KW - Adzuki bean beetle

KW - Remating inhibition

KW - Seminal fluid

KW - Testis

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