Female-biased sex difference in vasotocin-immunoreactive neural structures in the developing quail brain

Nicoletta Aste, Naoki Yoshioka, Emiko Sakamoto, Noboru Saito

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The bed nucleus of the stria terminalis pars medialis (BSTM), medial preoptic nucleus (POM), and lateral septal region (LS) exhibit more vasotocin-immunoreactive (VT-ir) neural structures in male than in female adult quail. VT-ir cells and fibers in these regions are sensitive to gonadal steroids only in males. The insensitivity of adult female VT-ir neural structures to sex steroids is attributed to estradiol exposure during a critical period in embryonic life.Although the VT-ir system has been intensively examined in adult quail, information is limited in embryos and juveniles. Therefore, we herein investigated the development of VT-immunoreactive neural structures from embryonic day (E) 9 to adulthood with a particular focus on the BSTM, POM and LS of both sexes.VT-ir neural structures were more evident in female than in male embryos from E9 (BSTM and POM) and E11 (LS). This sex difference disappeared between E15 and post-hatch day 1 in the BSTM and POM, and during the first week of life in the LS. Male-biased sex differences in VT-ir structures appeared at puberty. Female-biased sexual dimorphism in the density of the VT-ir structures of BSTM was reflected by the stronger expression of VT mRNA in females than in males. However, the density of VT mRNA somata was comparable in the two sexes.The exposure of male embryos to estradiol resulted in the feminization of VT-ir neural structures in the BSTM, but not in the POM or LS at E11.Collectively, these results suggest that sex differences in VT-ir neural structures changes drastically throughout quail life. In embryos, endogenous estradiol may stimulate the expression of VT in females, resulting in a robust sex difference in VT-ir cells and fibers in favor of this sex.

Original languageEnglish
Pages (from-to)41-54
Number of pages14
JournalJournal of Chemical Neuroanatomy
Volume77
DOIs
Publication statusPublished - Nov 1 2016
Externally publishedYes

Fingerprint

Vasotocin
Quail
Sex Characteristics
Septal Nuclei
Septum of Brain
Brain
Embryonic Structures
Estradiol
Steroids
Feminization
Messenger RNA
Preoptic Area
Carisoprodol
Puberty

Keywords

  • Bird
  • Brain
  • BSTM
  • Development
  • Sex dimorphism
  • Vasotocin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Female-biased sex difference in vasotocin-immunoreactive neural structures in the developing quail brain. / Aste, Nicoletta; Yoshioka, Naoki; Sakamoto, Emiko; Saito, Noboru.

In: Journal of Chemical Neuroanatomy, Vol. 77, 01.11.2016, p. 41-54.

Research output: Contribution to journalArticle

@article{ca70b9471224469092b5c903c66b8632,
title = "Female-biased sex difference in vasotocin-immunoreactive neural structures in the developing quail brain",
abstract = "The bed nucleus of the stria terminalis pars medialis (BSTM), medial preoptic nucleus (POM), and lateral septal region (LS) exhibit more vasotocin-immunoreactive (VT-ir) neural structures in male than in female adult quail. VT-ir cells and fibers in these regions are sensitive to gonadal steroids only in males. The insensitivity of adult female VT-ir neural structures to sex steroids is attributed to estradiol exposure during a critical period in embryonic life.Although the VT-ir system has been intensively examined in adult quail, information is limited in embryos and juveniles. Therefore, we herein investigated the development of VT-immunoreactive neural structures from embryonic day (E) 9 to adulthood with a particular focus on the BSTM, POM and LS of both sexes.VT-ir neural structures were more evident in female than in male embryos from E9 (BSTM and POM) and E11 (LS). This sex difference disappeared between E15 and post-hatch day 1 in the BSTM and POM, and during the first week of life in the LS. Male-biased sex differences in VT-ir structures appeared at puberty. Female-biased sexual dimorphism in the density of the VT-ir structures of BSTM was reflected by the stronger expression of VT mRNA in females than in males. However, the density of VT mRNA somata was comparable in the two sexes.The exposure of male embryos to estradiol resulted in the feminization of VT-ir neural structures in the BSTM, but not in the POM or LS at E11.Collectively, these results suggest that sex differences in VT-ir neural structures changes drastically throughout quail life. In embryos, endogenous estradiol may stimulate the expression of VT in females, resulting in a robust sex difference in VT-ir cells and fibers in favor of this sex.",
keywords = "Bird, Brain, BSTM, Development, Sex dimorphism, Vasotocin",
author = "Nicoletta Aste and Naoki Yoshioka and Emiko Sakamoto and Noboru Saito",
year = "2016",
month = "11",
day = "1",
doi = "10.1016/j.jchemneu.2016.05.002",
language = "English",
volume = "77",
pages = "41--54",
journal = "Journal of Chemical Neuroanatomy",
issn = "0891-0618",
publisher = "Elsevier",

}

TY - JOUR

T1 - Female-biased sex difference in vasotocin-immunoreactive neural structures in the developing quail brain

AU - Aste, Nicoletta

AU - Yoshioka, Naoki

AU - Sakamoto, Emiko

AU - Saito, Noboru

PY - 2016/11/1

Y1 - 2016/11/1

N2 - The bed nucleus of the stria terminalis pars medialis (BSTM), medial preoptic nucleus (POM), and lateral septal region (LS) exhibit more vasotocin-immunoreactive (VT-ir) neural structures in male than in female adult quail. VT-ir cells and fibers in these regions are sensitive to gonadal steroids only in males. The insensitivity of adult female VT-ir neural structures to sex steroids is attributed to estradiol exposure during a critical period in embryonic life.Although the VT-ir system has been intensively examined in adult quail, information is limited in embryos and juveniles. Therefore, we herein investigated the development of VT-immunoreactive neural structures from embryonic day (E) 9 to adulthood with a particular focus on the BSTM, POM and LS of both sexes.VT-ir neural structures were more evident in female than in male embryos from E9 (BSTM and POM) and E11 (LS). This sex difference disappeared between E15 and post-hatch day 1 in the BSTM and POM, and during the first week of life in the LS. Male-biased sex differences in VT-ir structures appeared at puberty. Female-biased sexual dimorphism in the density of the VT-ir structures of BSTM was reflected by the stronger expression of VT mRNA in females than in males. However, the density of VT mRNA somata was comparable in the two sexes.The exposure of male embryos to estradiol resulted in the feminization of VT-ir neural structures in the BSTM, but not in the POM or LS at E11.Collectively, these results suggest that sex differences in VT-ir neural structures changes drastically throughout quail life. In embryos, endogenous estradiol may stimulate the expression of VT in females, resulting in a robust sex difference in VT-ir cells and fibers in favor of this sex.

AB - The bed nucleus of the stria terminalis pars medialis (BSTM), medial preoptic nucleus (POM), and lateral septal region (LS) exhibit more vasotocin-immunoreactive (VT-ir) neural structures in male than in female adult quail. VT-ir cells and fibers in these regions are sensitive to gonadal steroids only in males. The insensitivity of adult female VT-ir neural structures to sex steroids is attributed to estradiol exposure during a critical period in embryonic life.Although the VT-ir system has been intensively examined in adult quail, information is limited in embryos and juveniles. Therefore, we herein investigated the development of VT-immunoreactive neural structures from embryonic day (E) 9 to adulthood with a particular focus on the BSTM, POM and LS of both sexes.VT-ir neural structures were more evident in female than in male embryos from E9 (BSTM and POM) and E11 (LS). This sex difference disappeared between E15 and post-hatch day 1 in the BSTM and POM, and during the first week of life in the LS. Male-biased sex differences in VT-ir structures appeared at puberty. Female-biased sexual dimorphism in the density of the VT-ir structures of BSTM was reflected by the stronger expression of VT mRNA in females than in males. However, the density of VT mRNA somata was comparable in the two sexes.The exposure of male embryos to estradiol resulted in the feminization of VT-ir neural structures in the BSTM, but not in the POM or LS at E11.Collectively, these results suggest that sex differences in VT-ir neural structures changes drastically throughout quail life. In embryos, endogenous estradiol may stimulate the expression of VT in females, resulting in a robust sex difference in VT-ir cells and fibers in favor of this sex.

KW - Bird

KW - Brain

KW - BSTM

KW - Development

KW - Sex dimorphism

KW - Vasotocin

UR - http://www.scopus.com/inward/record.url?scp=84969172347&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84969172347&partnerID=8YFLogxK

U2 - 10.1016/j.jchemneu.2016.05.002

DO - 10.1016/j.jchemneu.2016.05.002

M3 - Article

C2 - 27156479

AN - SCOPUS:84969172347

VL - 77

SP - 41

EP - 54

JO - Journal of Chemical Neuroanatomy

JF - Journal of Chemical Neuroanatomy

SN - 0891-0618

ER -