TY - JOUR
T1 - Fecal metabolite of a gnotobiotic mouse transplanted with gut microbiota from a patient with Alzheimer’s disease
AU - Fujii, Yusuke
AU - Nguyen, Thuy Tien Thi
AU - Fujimura, Yuta
AU - Kameya, Naotaka
AU - Nakamura, Shoji
AU - Arakawa, Kensuke
AU - Morita, Hidetoshi
N1 - Funding Information:
This work was supported by JSPS KAKENHI [grant number 16K15061].
Publisher Copyright:
© 2019 Japan Society for Bioscience, Biotechnology, and Agrochemistry
PY - 2019
Y1 - 2019
N2 - Studies of Alzheimer’s disease are based on model mice that have been altered by transgenesis and other techniques to elicit pathogenesis. However, changes in the gut microbiota were recently suggested to diminish cognitive function in patients, as well as in model mice. Accordingly, we have created model mice of the human gut microbiota by transplanting germ-free C57BL/6N mice with fecal samples from a healthy volunteer and from an affected patient. These humanized mice were stably colonized and reproduced the bacterial diversity in donors. Remarkably, performance on Object Location Test and Object Recognition Test was significantly reduced in the latter than in the former at 55 weeks of age, suggesting that gut microbiota transplanted from an affected patient affects mouse behavior. In addition, metabolites related to the nervous system, including γ-aminobutyrate, taurine, and valine, were significantly less abundant in the feces of mice transplanted with microbiota from the affected patient.
AB - Studies of Alzheimer’s disease are based on model mice that have been altered by transgenesis and other techniques to elicit pathogenesis. However, changes in the gut microbiota were recently suggested to diminish cognitive function in patients, as well as in model mice. Accordingly, we have created model mice of the human gut microbiota by transplanting germ-free C57BL/6N mice with fecal samples from a healthy volunteer and from an affected patient. These humanized mice were stably colonized and reproduced the bacterial diversity in donors. Remarkably, performance on Object Location Test and Object Recognition Test was significantly reduced in the latter than in the former at 55 weeks of age, suggesting that gut microbiota transplanted from an affected patient affects mouse behavior. In addition, metabolites related to the nervous system, including γ-aminobutyrate, taurine, and valine, were significantly less abundant in the feces of mice transplanted with microbiota from the affected patient.
KW - 16S rRNA gene sequencing
KW - Alzheimer’s disease
KW - Behavior
KW - Gut microbiota
KW - Metabolome
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U2 - 10.1080/09168451.2019.1644149
DO - 10.1080/09168451.2019.1644149
M3 - Article
C2 - 31327302
AN - SCOPUS:85073487273
VL - 83
SP - 2144
EP - 2152
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 11
ER -