Fecal metabolite of a gnotobiotic mouse transplanted with gut microbiota from a patient with Alzheimer’s disease

Yusuke Fujii, Thuy Tien Thi Nguyen, Yuta Fujimura, Naotaka Kameya, Shoji Nakamura, Kensuke Arakawa, Hidetoshi Morita

Research output: Contribution to journalArticle

Abstract

Studies of Alzheimer’s disease are based on model mice that have been altered by transgenesis and other techniques to elicit pathogenesis. However, changes in the gut microbiota were recently suggested to diminish cognitive function in patients, as well as in model mice. Accordingly, we have created model mice of the human gut microbiota by transplanting germ-free C57BL/6N mice with fecal samples from a healthy volunteer and from an affected patient. These humanized mice were stably colonized and reproduced the bacterial diversity in donors. Remarkably, performance on Object Location Test and Object Recognition Test was significantly reduced in the latter than in the former at 55 weeks of age, suggesting that gut microbiota transplanted from an affected patient affects mouse behavior. In addition, metabolites related to the nervous system, including γ-aminobutyrate, taurine, and valine, were significantly less abundant in the feces of mice transplanted with microbiota from the affected patient.

Original languageEnglish
Pages (from-to)2144-2152
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume83
Issue number11
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Germ-Free Life
Metabolites
Alzheimer Disease
Aminobutyrates
Taurine
Object recognition
Valine
Neurology
Gene Transfer Techniques
Microbiota
Inbred C57BL Mouse
Feces
Cognition
Nervous System
Gastrointestinal Microbiome
Healthy Volunteers
Tissue Donors

Keywords

  • 16S rRNA gene sequencing
  • Alzheimer’s disease
  • Behavior
  • Gut microbiota
  • Metabolome

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Fecal metabolite of a gnotobiotic mouse transplanted with gut microbiota from a patient with Alzheimer’s disease. / Fujii, Yusuke; Nguyen, Thuy Tien Thi; Fujimura, Yuta; Kameya, Naotaka; Nakamura, Shoji; Arakawa, Kensuke; Morita, Hidetoshi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 83, No. 11, 01.01.2019, p. 2144-2152.

Research output: Contribution to journalArticle

Fujii, Yusuke ; Nguyen, Thuy Tien Thi ; Fujimura, Yuta ; Kameya, Naotaka ; Nakamura, Shoji ; Arakawa, Kensuke ; Morita, Hidetoshi. / Fecal metabolite of a gnotobiotic mouse transplanted with gut microbiota from a patient with Alzheimer’s disease. In: Bioscience, Biotechnology and Biochemistry. 2019 ; Vol. 83, No. 11. pp. 2144-2152.
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