Abstract
In many mammals, endometrial cells are remodeled by apoptosis and cell proliferation throughout the estrous cycle. Although apoptosis is known to be induced by various factors involving two major apoptotic pathways (the death receptor- and mitochondria-mediated pathways), how it is regulated in the bovine endometrium is unclear. We examined (1) the cyclic expressions of apoptosis-related factors, FAS, DcR3, BCL2 and BAX, in the bovine endometrium and (2) the effect of death ligands on the viability of, and FAS mRNA expression in, cultured bovine endometrial epithelial and stromal cells. FAS expression did not change during the estrous cycle, whereas DcR3 expression was higher at the mid and late luteal stages than at the early luteal and follicular stages. BCL2 expression was higher at the late luteal stage than at the early luteal and follicular stages, and the BAX/BCL2 ratio was higher at the early luteal stage than at the late luteal stage. Treatment or pretreatment with tumor necrosis factor-α (TNF). +. interferon γ (IFNG) in combination with FAS ligand significantly reduced the viability of both epithelial and stromal cells. Furthermore, TNF. +. IFNG treatment significantly increased the expression of FAS mRNA in both types of endometrial cells. The overall results suggest that both extrinsic and intrinsic pathways are involved in remodeling the bovine endometrium throughout the estrous cycle, and that the death ligands produced by immune cells and the endometrium play important roles in inducing cyclic endometrial cell death.
| Original language | English |
|---|---|
| Pages (from-to) | 97-104 |
| Number of pages | 8 |
| Journal | Animal Reproduction Science |
| Volume | 151 |
| Issue number | 3-4 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Apoptosis
- Cattle
- Endometrium
- Remodeling
ASJC Scopus subject areas
- Animal Science and Zoology
- Food Animals
- Endocrinology
- Medicine(all)
Cite this
FAS/FASL-mediated cell death in the bovine endometrium. / Arai, Miki; Yoshioka, Shin; Nishimura, Ryo; Okuda, Kiyoshi.
In: Animal Reproduction Science, Vol. 151, No. 3-4, 2014, p. 97-104.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - FAS/FASL-mediated cell death in the bovine endometrium
AU - Arai, Miki
AU - Yoshioka, Shin
AU - Nishimura, Ryo
AU - Okuda, Kiyoshi
PY - 2014
Y1 - 2014
N2 - In many mammals, endometrial cells are remodeled by apoptosis and cell proliferation throughout the estrous cycle. Although apoptosis is known to be induced by various factors involving two major apoptotic pathways (the death receptor- and mitochondria-mediated pathways), how it is regulated in the bovine endometrium is unclear. We examined (1) the cyclic expressions of apoptosis-related factors, FAS, DcR3, BCL2 and BAX, in the bovine endometrium and (2) the effect of death ligands on the viability of, and FAS mRNA expression in, cultured bovine endometrial epithelial and stromal cells. FAS expression did not change during the estrous cycle, whereas DcR3 expression was higher at the mid and late luteal stages than at the early luteal and follicular stages. BCL2 expression was higher at the late luteal stage than at the early luteal and follicular stages, and the BAX/BCL2 ratio was higher at the early luteal stage than at the late luteal stage. Treatment or pretreatment with tumor necrosis factor-α (TNF). +. interferon γ (IFNG) in combination with FAS ligand significantly reduced the viability of both epithelial and stromal cells. Furthermore, TNF. +. IFNG treatment significantly increased the expression of FAS mRNA in both types of endometrial cells. The overall results suggest that both extrinsic and intrinsic pathways are involved in remodeling the bovine endometrium throughout the estrous cycle, and that the death ligands produced by immune cells and the endometrium play important roles in inducing cyclic endometrial cell death.
AB - In many mammals, endometrial cells are remodeled by apoptosis and cell proliferation throughout the estrous cycle. Although apoptosis is known to be induced by various factors involving two major apoptotic pathways (the death receptor- and mitochondria-mediated pathways), how it is regulated in the bovine endometrium is unclear. We examined (1) the cyclic expressions of apoptosis-related factors, FAS, DcR3, BCL2 and BAX, in the bovine endometrium and (2) the effect of death ligands on the viability of, and FAS mRNA expression in, cultured bovine endometrial epithelial and stromal cells. FAS expression did not change during the estrous cycle, whereas DcR3 expression was higher at the mid and late luteal stages than at the early luteal and follicular stages. BCL2 expression was higher at the late luteal stage than at the early luteal and follicular stages, and the BAX/BCL2 ratio was higher at the early luteal stage than at the late luteal stage. Treatment or pretreatment with tumor necrosis factor-α (TNF). +. interferon γ (IFNG) in combination with FAS ligand significantly reduced the viability of both epithelial and stromal cells. Furthermore, TNF. +. IFNG treatment significantly increased the expression of FAS mRNA in both types of endometrial cells. The overall results suggest that both extrinsic and intrinsic pathways are involved in remodeling the bovine endometrium throughout the estrous cycle, and that the death ligands produced by immune cells and the endometrium play important roles in inducing cyclic endometrial cell death.
KW - Apoptosis
KW - Cattle
KW - Endometrium
KW - Remodeling
UR - http://www.scopus.com/inward/record.url?scp=84922741949&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84922741949&partnerID=8YFLogxK
U2 - 10.1016/j.anireprosci.2014.10.004
DO - 10.1016/j.anireprosci.2014.10.004
M3 - Article
C2 - 25458316
AN - SCOPUS:84922741949
VL - 151
SP - 97
EP - 104
JO - Animal Reproduction Science
JF - Animal Reproduction Science
SN - 0378-4320
IS - 3-4
ER -