Fas-mediated apoptosis is suppressed by calf serum in cultured bovine luteal cells.

Dariusz J. Skarzynski, Masami Shibaya, Yukari Tasaki, Anna Korzekwa, Shuko Murakami, Izabela Woclawek-Potocka, Magdalena Majewska, Kiyoshi Okuda

Research output: Contribution to journalArticle

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Abstract

Calf serum (CS) is a common supplement used in cell culture. It has been suggested that CS contains substances protecting cells against apoptosis. To examine whether a culture system including CS is appropriate for studying apoptosis in bovine luteal cells, we examined the influence of CS on the expression of Fas, bcl-2 and bax gene. Since progesterone (P(4)) is known to be an anti-apoptotic factor in bovine luteal cells, the present study was carried out to examine the P(4) effect on apoptosis. Bovine mid-luteal cells were exposed to Fas ligand (Fas L) in the presence or in the absence of P(4) antagonist (onapristone, OP) in a basal medium (BM) containing 5% CS (BM-CS) or BM containing 0.1% BSA (BM-BSA). Although Fas L alone, OP alone or Fas L plus OP did not show any cytotoxic effect on the cells cultured in BM-CS, administration of OP or OP in combination with Fas L resulted in the killing of 30% and 55% of the cells cultured in BM-BSA medium, respectively (p

Original languageEnglish
Pages (from-to)3-15
Number of pages13
JournalReproductive biology
Volume7
Issue number1
Publication statusPublished - Mar 2007
Externally publishedYes

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Luteal Cells
luteal cells
apoptosis
calves
Apoptosis
cattle
Serum
cultured cells
Cultured Cells
bcl-2 Genes
Fas Ligand Protein
Progesterone
cytotoxicity
antagonists
progesterone
cell culture
Cell Culture Techniques
onapristone

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Skarzynski, D. J., Shibaya, M., Tasaki, Y., Korzekwa, A., Murakami, S., Woclawek-Potocka, I., ... Okuda, K. (2007). Fas-mediated apoptosis is suppressed by calf serum in cultured bovine luteal cells. Reproductive biology, 7(1), 3-15.

Fas-mediated apoptosis is suppressed by calf serum in cultured bovine luteal cells. / Skarzynski, Dariusz J.; Shibaya, Masami; Tasaki, Yukari; Korzekwa, Anna; Murakami, Shuko; Woclawek-Potocka, Izabela; Majewska, Magdalena; Okuda, Kiyoshi.

In: Reproductive biology, Vol. 7, No. 1, 03.2007, p. 3-15.

Research output: Contribution to journalArticle

Skarzynski, DJ, Shibaya, M, Tasaki, Y, Korzekwa, A, Murakami, S, Woclawek-Potocka, I, Majewska, M & Okuda, K 2007, 'Fas-mediated apoptosis is suppressed by calf serum in cultured bovine luteal cells.', Reproductive biology, vol. 7, no. 1, pp. 3-15.
Skarzynski DJ, Shibaya M, Tasaki Y, Korzekwa A, Murakami S, Woclawek-Potocka I et al. Fas-mediated apoptosis is suppressed by calf serum in cultured bovine luteal cells. Reproductive biology. 2007 Mar;7(1):3-15.
Skarzynski, Dariusz J. ; Shibaya, Masami ; Tasaki, Yukari ; Korzekwa, Anna ; Murakami, Shuko ; Woclawek-Potocka, Izabela ; Majewska, Magdalena ; Okuda, Kiyoshi. / Fas-mediated apoptosis is suppressed by calf serum in cultured bovine luteal cells. In: Reproductive biology. 2007 ; Vol. 7, No. 1. pp. 3-15.
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