Facilitation of glutamate release by ionotropic glutamate receptors in osteoblasts

Eiichi Hinoi, Sayumi Fujimori, Takeshi Takarada, Hideo Taniura, Yukio Yoneda

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Constitutive expression of mRNA was seen for the vesicular glutamate transporter brain-specific Na+-dependent inorganic phosphate cotransporter (BNPI), but not differentiation-associated Na+-dependent inorganic phosphate cotransporter, in rat calvarial osteoblasts cultured for 7 and 21 days in vitro (DIV). Three different agonists for ionotropic glutamate receptors (iGluR) at 1 mM, as well as 50 mM KCl, significantly increased the release of endogenous L-glutamate from osteoblasts cultured for 7 DIV when determined 5 min after the addition by using a high performance liquid chromatograph. The inhibitor of desensitization of DL-α-amino-3-hydroxy-5-methylisoxasole-4-propionate (AMPA) receptors cyclothiazide significantly potentiated and prolonged the release of endogenous L-glutamate evoked by AMPA in a dose-dependent manner. The release evoked by AMPA was significantly prevented by the addition of an AMPA receptor antagonist as well as by the removal of Ca2+ ions. These results suggest that endogenous L-glutamate could be released from intracellular vesicular constituents associated with BNPI through activation of particular iGluR subtypes expressed in cultured rat calvarial osteoblasts.

Original languageEnglish
Pages (from-to)452-458
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume297
Issue number3
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Ionotropic Glutamate Receptors
Osteoblasts
Glutamic Acid
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
AMPA Receptors
Rats
Vesicular Glutamate Transport Protein 1
Vesicular Glutamate Transport Protein 2
Vesicular Glutamate Transport Proteins
Propionates
Chemical activation
Ions
Messenger RNA
Liquids
In Vitro Techniques

Keywords

  • AMPA receptors
  • BNPI
  • Exocytosis
  • Glutamate
  • Osteoblasts
  • Release
  • Vesicular transporter

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Facilitation of glutamate release by ionotropic glutamate receptors in osteoblasts. / Hinoi, Eiichi; Fujimori, Sayumi; Takarada, Takeshi; Taniura, Hideo; Yoneda, Yukio.

In: Biochemical and Biophysical Research Communications, Vol. 297, No. 3, 2002, p. 452-458.

Research output: Contribution to journalArticle

Hinoi, Eiichi ; Fujimori, Sayumi ; Takarada, Takeshi ; Taniura, Hideo ; Yoneda, Yukio. / Facilitation of glutamate release by ionotropic glutamate receptors in osteoblasts. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 297, No. 3. pp. 452-458.
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