Abstract
Constitutive expression of mRNA was seen for the vesicular glutamate transporter brain-specific Na+-dependent inorganic phosphate cotransporter (BNPI), but not differentiation-associated Na+-dependent inorganic phosphate cotransporter, in rat calvarial osteoblasts cultured for 7 and 21 days in vitro (DIV). Three different agonists for ionotropic glutamate receptors (iGluR) at 1 mM, as well as 50 mM KCl, significantly increased the release of endogenous L-glutamate from osteoblasts cultured for 7 DIV when determined 5 min after the addition by using a high performance liquid chromatograph. The inhibitor of desensitization of DL-α-amino-3-hydroxy-5-methylisoxasole-4-propionate (AMPA) receptors cyclothiazide significantly potentiated and prolonged the release of endogenous L-glutamate evoked by AMPA in a dose-dependent manner. The release evoked by AMPA was significantly prevented by the addition of an AMPA receptor antagonist as well as by the removal of Ca2+ ions. These results suggest that endogenous L-glutamate could be released from intracellular vesicular constituents associated with BNPI through activation of particular iGluR subtypes expressed in cultured rat calvarial osteoblasts.
| Original language | English |
|---|---|
| Pages (from-to) | 452-458 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 297 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2002 |
| Externally published | Yes |
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Keywords
- AMPA receptors
- BNPI
- Exocytosis
- Glutamate
- Osteoblasts
- Release
- Vesicular transporter
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Facilitation of glutamate release by ionotropic glutamate receptors in osteoblasts. / Hinoi, Eiichi; Fujimori, Sayumi; Takarada, Takeshi; Taniura, Hideo; Yoneda, Yukio.
In: Biochemical and Biophysical Research Communications, Vol. 297, No. 3, 2002, p. 452-458.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Facilitation of glutamate release by ionotropic glutamate receptors in osteoblasts
AU - Hinoi, Eiichi
AU - Fujimori, Sayumi
AU - Takarada, Takeshi
AU - Taniura, Hideo
AU - Yoneda, Yukio
PY - 2002
Y1 - 2002
N2 - Constitutive expression of mRNA was seen for the vesicular glutamate transporter brain-specific Na+-dependent inorganic phosphate cotransporter (BNPI), but not differentiation-associated Na+-dependent inorganic phosphate cotransporter, in rat calvarial osteoblasts cultured for 7 and 21 days in vitro (DIV). Three different agonists for ionotropic glutamate receptors (iGluR) at 1 mM, as well as 50 mM KCl, significantly increased the release of endogenous L-glutamate from osteoblasts cultured for 7 DIV when determined 5 min after the addition by using a high performance liquid chromatograph. The inhibitor of desensitization of DL-α-amino-3-hydroxy-5-methylisoxasole-4-propionate (AMPA) receptors cyclothiazide significantly potentiated and prolonged the release of endogenous L-glutamate evoked by AMPA in a dose-dependent manner. The release evoked by AMPA was significantly prevented by the addition of an AMPA receptor antagonist as well as by the removal of Ca2+ ions. These results suggest that endogenous L-glutamate could be released from intracellular vesicular constituents associated with BNPI through activation of particular iGluR subtypes expressed in cultured rat calvarial osteoblasts.
AB - Constitutive expression of mRNA was seen for the vesicular glutamate transporter brain-specific Na+-dependent inorganic phosphate cotransporter (BNPI), but not differentiation-associated Na+-dependent inorganic phosphate cotransporter, in rat calvarial osteoblasts cultured for 7 and 21 days in vitro (DIV). Three different agonists for ionotropic glutamate receptors (iGluR) at 1 mM, as well as 50 mM KCl, significantly increased the release of endogenous L-glutamate from osteoblasts cultured for 7 DIV when determined 5 min after the addition by using a high performance liquid chromatograph. The inhibitor of desensitization of DL-α-amino-3-hydroxy-5-methylisoxasole-4-propionate (AMPA) receptors cyclothiazide significantly potentiated and prolonged the release of endogenous L-glutamate evoked by AMPA in a dose-dependent manner. The release evoked by AMPA was significantly prevented by the addition of an AMPA receptor antagonist as well as by the removal of Ca2+ ions. These results suggest that endogenous L-glutamate could be released from intracellular vesicular constituents associated with BNPI through activation of particular iGluR subtypes expressed in cultured rat calvarial osteoblasts.
KW - AMPA receptors
KW - BNPI
KW - Exocytosis
KW - Glutamate
KW - Osteoblasts
KW - Release
KW - Vesicular transporter
UR - http://www.scopus.com/inward/record.url?scp=0036387153&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036387153&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(02)02223-4
DO - 10.1016/S0006-291X(02)02223-4
M3 - Article
C2 - 12270113
AN - SCOPUS:0036387153
VL - 297
SP - 452
EP - 458
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -