Fabrication of hydroxyapatite ultra-thin layer on gold surface and its application for quartz crystal microbalance technique

Akira Monkawa, Toshiyuki Ikoma, Shunji Yunoki, Tomohiko Yoshioka, Junzo Tanaka, Dinko Chakarov, Bengt Kasemo

Research output: Contribution to journalArticle

76 Citations (Scopus)


We present a method for coating gold quartz crystal microbalance with dissipation (QCM-D) sensor with ultra-thin layer of hydroxyapatite nanocrystals evenly covering and tightly bound to the surface. The hydroxyapatite layer shows a plate-like morphology and less than 20 nm in thickness. The hydroxyapatite sensor operated in liquid with high stability and sensitivity. The in-situ adsorption mechanism and conformational change of fibrinogen on gold, titanium and hydroxyapatite surfaces were investigated by QCM-D technique and Fourier-transform infrared spectroscopy. The change of secondary structures of fibrinogen adsorbed on the surfaces depended on the adsorbed amounts of protein. The secondary structure of fibrinogen adsorbed on the surfaces changes with increasing coverage. This is explained by repulsion among fibrinogens, affecting water structure and thus the strength of fibrinogen interactions on the surface. The study indicates that the hydroxyapatite sensor is applicable for qualitative and conformational analysis of protein adsorption.

Original languageEnglish
Pages (from-to)5748-5754
Number of pages7
Issue number33
Publication statusPublished - Nov 2006
Externally publishedYes



  • Adsorption
  • Fibrinogen
  • FT-IR
  • Hydroxyapatite
  • QCM-D

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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