Fabrication of endothelialized tube in collagen gel as starting point for self-developing capillary-like network to construct three-dimensional organs in vitro

Takayuki Takei, Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A possible strategy for creating three-dimensional (3D) tissue-engineered organs in vitro with similar volumes to the primary organs is to develop a capillary network throughout the constructs to provide sufficient oxygenation and nutrition to the cells composing them. Here, we propose a novel approach for the creation of a capillary-like network in vitro, based on the spontaneous tube-forming activity of vascular endothelial cells (ECs) in collagen gel. We fabricated a linear tube of 500 μm in diameter, the inner surface of which was filled with bovine carotid artery vascular endothelial cells (BECs), in type I collagen gel as a starting point for the formation of a capillary-like network. The BECs exposed to a medium containing vascular endothelial growth factor (VEGF) migrated into the ambient gel around the tube. After 2 weeks of VEGF exposure, the distance of the migration into the ambient gel in the radial direction of the tube reached approximately 800 μm. Cross-sections of capillary-like structures composed of the migrating BECs, with a lumen-like interior space, were observed in slices of the gel around the tube stained with hematoxylin-eosin (H&E). These results demonstrate that this approach using a pre-established tube, which is composed of ECs, as a starting point for a self-developing capillary-like network is potentially useful for constructing 3D organs in vitro.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalBiotechnology and Bioengineering
Volume95
Issue number1
DOIs
Publication statusPublished - Sep 5 2006
Externally publishedYes

Fingerprint

Collagen
Gels
Endothelial cells
Fabrication
Endothelial Cells
Vascular Endothelial Growth Factor A
Oxygenation
Hematoxylin
Eosine Yellowish-(YS)
Nutrition
Collagen Type I
Carotid Arteries
In Vitro Techniques
Tissue
Intercellular Signaling Peptides and Proteins

Keywords

  • Angiogenesis
  • Capillary-like network
  • Collagen gel
  • Endothelial cell
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Fabrication of endothelialized tube in collagen gel as starting point for self-developing capillary-like network to construct three-dimensional organs in vitro. / Takei, Takayuki; Sakai, Shinji; Ono, Tsutomu; Ijima, Hiroyuki; Kawakami, Koei.

In: Biotechnology and Bioengineering, Vol. 95, No. 1, 05.09.2006, p. 1-7.

Research output: Contribution to journalArticle

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