Fabrication of biomimetic bone tissue using mesenchymal stem cell-derived three-dimensional constructs incorporating endothelial cells

Jun Ichi Sasaki, Masanori Hashimoto, Satoshi Yamaguchi, Yoshihiro Itoh, Itsumi Yoshimoto, Takuya Matsumoto, Satoshi Imazato

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The development of technologies to promote vascularization of engineered tissue would drive major developments in tissue engineering and regenerative medicine. Recently, we succeeded in fabricating three-dimensional (3D) cell constructs composed of mesenchymal stem cells (MSCs). However, the majority of cells within the constructs underwent necrosis due to a lack of nutrients and oxygen. We hypothesized that incorporation of vascular endothelial cells would improve the cell survival rate and aid in the fabrication of biomimetic bone tissues in vitro. The purpose of this study was to assess the impact of endothelial cells combined with the MSC constructs (MSC/HUVEC constructs) during short- and long-term culture. When human umbilical vein endothelial cells (HUVECs) were incorporated into the cell constructs, cell viability and growth factor production were increased after 7 days. Furthermore, HUVECs were observed to proliferate and self-organize into reticulate porous structures by interacting with the MSCs. After long-term culture, MSC/HUVEC constructs formed abundant mineralized matrices compared with those composed of MSCs alone. Transmission electron microscopy and qualitative analysis revealed that the mineralized matrices comprised porous cancellous bone-like tissues. These results demonstrate that highly biomimetic bone tissue can be fabricated in vitro by 3D MSC constructs incorporated with HUVECs.

Original languageEnglish
Article numbere0129266
JournalPLoS One
Volume10
Issue number6
DOIs
Publication statusPublished - Jun 5 2015

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biomimetics
Biomimetics
Endothelial cells
Stem cells
Mesenchymal Stromal Cells
endothelial cells
stem cells
Bone
Human Umbilical Vein Endothelial Cells
Endothelial Cells
bones
Tissue
Bone and Bones
Fabrication
Cell culture
cell viability
Cell Survival
Cells
tissue engineering
Regenerative Medicine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Fabrication of biomimetic bone tissue using mesenchymal stem cell-derived three-dimensional constructs incorporating endothelial cells. / Sasaki, Jun Ichi; Hashimoto, Masanori; Yamaguchi, Satoshi; Itoh, Yoshihiro; Yoshimoto, Itsumi; Matsumoto, Takuya; Imazato, Satoshi.

In: PLoS One, Vol. 10, No. 6, e0129266, 05.06.2015.

Research output: Contribution to journalArticle

Sasaki, Jun Ichi ; Hashimoto, Masanori ; Yamaguchi, Satoshi ; Itoh, Yoshihiro ; Yoshimoto, Itsumi ; Matsumoto, Takuya ; Imazato, Satoshi. / Fabrication of biomimetic bone tissue using mesenchymal stem cell-derived three-dimensional constructs incorporating endothelial cells. In: PLoS One. 2015 ; Vol. 10, No. 6.
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