Fabrication of benzylamine-terminated surfaces onto glass substrates for detection of catecholamines by fluorogenic derivatization

Nobuko Fukuda, Kazuma Tsuboi, Hirobumi Ushijima

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

We achieved fabrication of the benzylamine-terminated surface onto a glass substrate for detection of catecholamines by fluorogenic derivatization. Fabrication was carried out in turn as follows, (1) immobilization of 3-aminopropyltriethoxysilane (APS) onto the glass surface, (2) modification of 1,4-phenylenediisothiocyanate (PDC) onto the amino-terminated surface, and (3) modification of p-xylylenediamine (XDA) onto the isothiocyanate-terminated surface. The sequent surface reactions were monitored by UV-vis spectroscopy, contact angle measurement, X-ray photoelectron spectroscopy (XPS), and surface plasmon spectroscopy. Fluorogenic derivatization of adrenaline was performed by exposure of the benzylamine-terminated surface to borate buffer containing adrenaline and potassium hexacyanoferrate (III) as an oxidant. Fluorescent spectra indicated existence of a fluorophore on the benzylamine-terminated surface after treatment with adrenaline.

Original languageEnglish
Pages (from-to)502-506
Number of pages5
JournalSensor Letters
Volume6
Issue number4
DOIs
Publication statusPublished - Aug 2008
Externally publishedYes

Keywords

  • Benzylamine
  • Catecholamine
  • Derivatization
  • Fluorogenic
  • Surface reaction
  • Ubiquitous mental health care system

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

Fingerprint

Dive into the research topics of 'Fabrication of benzylamine-terminated surfaces onto glass substrates for detection of catecholamines by fluorogenic derivatization'. Together they form a unique fingerprint.

Cite this