Fabrication of a silica monolith micro-bioreactor entrapping highly activated lipase by a two-step sol-gel method

K. Kawakami, D. Abe, T. Urakawa, S. Sakai, Tsutomu Ono, H. Ijima

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Micro-bioreactors are effective for high throughput production of expensive products from small amounts of substrates. Lipases are versatile enzymes for chiral syntheses, and highly activated when immobilized in alkyl-substituted silicates by the sol-gel method. For application of the immobilized lipases to a continuous-flow system, a micro-bioreactor loaded with a macroporous silica monolith is well suited, because it can easily be integrated with a separator for optical resolution. We attempted to develop a micro-capillary bioreactor containing a silica monolith-immobilized lipase. A silica monolith without shrinkage during lyophilization of the hydrogel was first formed from a 1:4 mixture of tetramethoxysilane (TMOS) and methyltrimethoxysilane (MTMS). It was then coated with silica precipitates entrapping lipase, derived from a 1:4 mixture of TMOS and n-butyltrimethoxysilane (BTMS). As a result, the monolith treated with the BTMS-based silica entrapping lipase, exhibited approximately ten times higher esterification activity than the non-treated monolith-immobilized lipase derived from the MTMS-based silica.

Original languageEnglish
Title of host publicationCHISA 2006 - 17th International Congress of Chemical and Process Engineering
Publication statusPublished - 2006
Externally publishedYes
EventCHISA 2006 - 17th International Congress of Chemical and Process Engineering - Prague, Czech Republic
Duration: Aug 27 2006Aug 31 2006

Other

OtherCHISA 2006 - 17th International Congress of Chemical and Process Engineering
CountryCzech Republic
CityPrague
Period8/27/068/31/06

Fingerprint

Lipases
Bioreactors
Lipase
Silicon Dioxide
Sol-gel process
Silica
Fabrication
Silicates
Hydrogel
Esterification
Separators
Hydrogels
Precipitates
Enzymes
Throughput
Substrates

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Kawakami, K., Abe, D., Urakawa, T., Sakai, S., Ono, T., & Ijima, H. (2006). Fabrication of a silica monolith micro-bioreactor entrapping highly activated lipase by a two-step sol-gel method. In CHISA 2006 - 17th International Congress of Chemical and Process Engineering

Fabrication of a silica monolith micro-bioreactor entrapping highly activated lipase by a two-step sol-gel method. / Kawakami, K.; Abe, D.; Urakawa, T.; Sakai, S.; Ono, Tsutomu; Ijima, H.

CHISA 2006 - 17th International Congress of Chemical and Process Engineering. 2006.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kawakami, K, Abe, D, Urakawa, T, Sakai, S, Ono, T & Ijima, H 2006, Fabrication of a silica monolith micro-bioreactor entrapping highly activated lipase by a two-step sol-gel method. in CHISA 2006 - 17th International Congress of Chemical and Process Engineering. CHISA 2006 - 17th International Congress of Chemical and Process Engineering, Prague, Czech Republic, 8/27/06.
Kawakami K, Abe D, Urakawa T, Sakai S, Ono T, Ijima H. Fabrication of a silica monolith micro-bioreactor entrapping highly activated lipase by a two-step sol-gel method. In CHISA 2006 - 17th International Congress of Chemical and Process Engineering. 2006
Kawakami, K. ; Abe, D. ; Urakawa, T. ; Sakai, S. ; Ono, Tsutomu ; Ijima, H. / Fabrication of a silica monolith micro-bioreactor entrapping highly activated lipase by a two-step sol-gel method. CHISA 2006 - 17th International Congress of Chemical and Process Engineering. 2006.
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AU - Ono, Tsutomu

AU - Ijima, H.

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N2 - Micro-bioreactors are effective for high throughput production of expensive products from small amounts of substrates. Lipases are versatile enzymes for chiral syntheses, and highly activated when immobilized in alkyl-substituted silicates by the sol-gel method. For application of the immobilized lipases to a continuous-flow system, a micro-bioreactor loaded with a macroporous silica monolith is well suited, because it can easily be integrated with a separator for optical resolution. We attempted to develop a micro-capillary bioreactor containing a silica monolith-immobilized lipase. A silica monolith without shrinkage during lyophilization of the hydrogel was first formed from a 1:4 mixture of tetramethoxysilane (TMOS) and methyltrimethoxysilane (MTMS). It was then coated with silica precipitates entrapping lipase, derived from a 1:4 mixture of TMOS and n-butyltrimethoxysilane (BTMS). As a result, the monolith treated with the BTMS-based silica entrapping lipase, exhibited approximately ten times higher esterification activity than the non-treated monolith-immobilized lipase derived from the MTMS-based silica.

AB - Micro-bioreactors are effective for high throughput production of expensive products from small amounts of substrates. Lipases are versatile enzymes for chiral syntheses, and highly activated when immobilized in alkyl-substituted silicates by the sol-gel method. For application of the immobilized lipases to a continuous-flow system, a micro-bioreactor loaded with a macroporous silica monolith is well suited, because it can easily be integrated with a separator for optical resolution. We attempted to develop a micro-capillary bioreactor containing a silica monolith-immobilized lipase. A silica monolith without shrinkage during lyophilization of the hydrogel was first formed from a 1:4 mixture of tetramethoxysilane (TMOS) and methyltrimethoxysilane (MTMS). It was then coated with silica precipitates entrapping lipase, derived from a 1:4 mixture of TMOS and n-butyltrimethoxysilane (BTMS). As a result, the monolith treated with the BTMS-based silica entrapping lipase, exhibited approximately ten times higher esterification activity than the non-treated monolith-immobilized lipase derived from the MTMS-based silica.

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