Ezrin enrichment on curved membranes requires a specific conformation or interaction with a curvature-sensitive partner

Feng Ching Tsai, Aurelie Bertin, Hugo Bousquet, John Manzi, Yosuke Senju, Meng Chen Tsai, Laura Picas, Stephanie Miserey-Lenkei, Pekka Lappalainen, Emmanuel Lemichez, Evelyne Coudrier, Patricia Bassereau

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

One challenge in cell biology is to decipher the biophysical mechanisms governing protein enrichment on curved membranes and the resulting membrane deformation. The ERM protein ezrin is abundant and associated with cellular membranes that are flat, positively or negatively curved. Using in vitro and cell biology approaches, we assess mechanisms of ezrin’s enrichment on curved membranes. We evidence that wild-type ezrin (ezrinWT) and its phosphomimetic mutant T567D (ezrinTD) do not deform membranes but self-assemble anti-parallelly, zipping adjacent membranes. EzrinTD’s specific conformation reduces intermolecular interactions, allows binding to actin filaments, which reduces membrane tethering, and promotes ezrin binding to positively-curved membranes. While neither ezrinTD nor ezrinWT senses negative curvature alone, we demonstrate that interacting with curvature-sensing I-BAR-domain proteins facilitates ezrin enrichment in negatively-curved membrane protrusions. Overall, our work demonstrates that ezrin can tether membranes, or be targeted to curved membranes, depending on conformations and interactions with actin and curvature-sensing binding partners.

Original languageEnglish
Article numbere37262
JournaleLife
Volume7
DOIs
Publication statusPublished - Sep 2018
Externally publishedYes

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Conformations
Membranes
Cytology
Cell Biology
Actins
ezrin
Proteins
Actin Cytoskeleton

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Ezrin enrichment on curved membranes requires a specific conformation or interaction with a curvature-sensitive partner. / Tsai, Feng Ching; Bertin, Aurelie; Bousquet, Hugo; Manzi, John; Senju, Yosuke; Tsai, Meng Chen; Picas, Laura; Miserey-Lenkei, Stephanie; Lappalainen, Pekka; Lemichez, Emmanuel; Coudrier, Evelyne; Bassereau, Patricia.

In: eLife, Vol. 7, e37262, 09.2018.

Research output: Contribution to journalArticle

Tsai, FC, Bertin, A, Bousquet, H, Manzi, J, Senju, Y, Tsai, MC, Picas, L, Miserey-Lenkei, S, Lappalainen, P, Lemichez, E, Coudrier, E & Bassereau, P 2018, 'Ezrin enrichment on curved membranes requires a specific conformation or interaction with a curvature-sensitive partner', eLife, vol. 7, e37262. https://doi.org/10.7554/eLife.37262
Tsai, Feng Ching ; Bertin, Aurelie ; Bousquet, Hugo ; Manzi, John ; Senju, Yosuke ; Tsai, Meng Chen ; Picas, Laura ; Miserey-Lenkei, Stephanie ; Lappalainen, Pekka ; Lemichez, Emmanuel ; Coudrier, Evelyne ; Bassereau, Patricia. / Ezrin enrichment on curved membranes requires a specific conformation or interaction with a curvature-sensitive partner. In: eLife. 2018 ; Vol. 7.
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