TY - JOUR
T1 - Ezrin enhances line tension along transcellular tunnel edges via NMIIa driven actomyosin cable formation
AU - Stefani, Caroline
AU - Gonzalez-Rodriguez, David
AU - Senju, Yosuke
AU - Doye, Anne
AU - Efimova, Nadia
AU - Janel, Sébastien
AU - Lipuma, Justine
AU - Tsai, Meng Chen
AU - Hamaoui, Daniel
AU - Maddugoda, Madhavi P.
AU - Cochet-Escartin, Olivier
AU - Prévost, Coline
AU - Lafont, Frank
AU - Svitkina, Tatyana
AU - Lappalainen, Pekka
AU - Bassereau, Patricia
AU - Lemichez, Emmanuel
N1 - Publisher Copyright:
© 2017 The Author(s).
PY - 2017/6/23
Y1 - 2017/6/23
N2 - Transendothelial cell macroaperture (TEM) tunnels control endothelium barrier function and are triggered by several toxins from pathogenic bacteria that provoke vascular leakage. Cellular dewetting theory predicted that a line tension of uncharacterized origin works at TEM boundaries to limit their widening. Here, by conducting high-resolution microscopy approaches we unveil the presence of an actomyosin cable encircling TEMs. We develop a theoretical cellular dewetting framework to interpret TEM physical parameters that are quantitatively determined by laser ablation experiments. This establishes the critical role of ezrin and non-muscle myosin II (NMII) in the progressive implementation of line tension. Mechanistically, fluorescence-recovery-after-photobleaching experiments point for the upstream role of ezrin in stabilizing actin filaments at the edges of TEMs, thereby favouring their crosslinking by NMIIa. Collectively, our findings ascribe to ezrin and NMIIa a critical function of enhancing line tension at the cell boundary surrounding the TEMs by promoting the formation of an actomyosin ring.
AB - Transendothelial cell macroaperture (TEM) tunnels control endothelium barrier function and are triggered by several toxins from pathogenic bacteria that provoke vascular leakage. Cellular dewetting theory predicted that a line tension of uncharacterized origin works at TEM boundaries to limit their widening. Here, by conducting high-resolution microscopy approaches we unveil the presence of an actomyosin cable encircling TEMs. We develop a theoretical cellular dewetting framework to interpret TEM physical parameters that are quantitatively determined by laser ablation experiments. This establishes the critical role of ezrin and non-muscle myosin II (NMII) in the progressive implementation of line tension. Mechanistically, fluorescence-recovery-after-photobleaching experiments point for the upstream role of ezrin in stabilizing actin filaments at the edges of TEMs, thereby favouring their crosslinking by NMIIa. Collectively, our findings ascribe to ezrin and NMIIa a critical function of enhancing line tension at the cell boundary surrounding the TEMs by promoting the formation of an actomyosin ring.
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U2 - 10.1038/ncomms15839
DO - 10.1038/ncomms15839
M3 - Article
C2 - 28643776
AN - SCOPUS:85021345811
VL - 8
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
M1 - 15839
ER -