As rich in polyunsaturated fatty acid (PUFA), sea urchin gonad could be used as a potential resource of such type ingredients. In the present study, lipid was extracted from sea urchin (Strongylocentrotus nudus) gonad, without using organic solvent but using an enzyme-assisted aqueous method and a supercritical carbon dioxide (SC-CO2) method. The yield of lipid extracted was calculated in comparison with the soxhlet extraction using ethyl ether as solvent. The results showed that enzyme-assisted aqueous extraction allowed recovering 82.0 ± 2.1,76.0 ± 2.3,72.9 ± 3.6, and 61.5 ± 1.6% of sea urchin gonad lipid from the sample hydrolyzed by papain, neutral protease, alkaline protease, and trypsin, respectively. Meanwhile, a lipid yield of 53.7 ± 2.6% was achieved by SC-CO2 extraction. The lipid obtained was divided into unsaponifiable (sterol) and fatty acid, and analyzed by GC-MS. Results demonstrated that the compositions of sterol and fatty acid were very similar for samples extracted by different methods. PUFA was the chief component which accounts for approximately 35% of the total fatty acid.
- Enzyme-assisted aqueous extraction
- Fatty acid
- Sea urchin (Strongylocentrotus nudus)
- Supercritical carbon dioxide extraction
ASJC Scopus subject areas
- Food Science
- Industrial and Manufacturing Engineering