Extraction of lipid from sea urchin (Strongylocentrotus nudus) gonad by enzyme-assisted aqueous and supercritical carbon dioxide methods

Bei Wei Zhu, Lei Qin, Da Yong Zhou, Hai Tao Wu, Jing Wu, Jing Feng Yang, Dong Mei Li, Xiu Ping Dong, Yoshiyuki Murata

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

As rich in polyunsaturated fatty acid (PUFA), sea urchin gonad could be used as a potential resource of such type ingredients. In the present study, lipid was extracted from sea urchin (Strongylocentrotus nudus) gonad, without using organic solvent but using an enzyme-assisted aqueous method and a supercritical carbon dioxide (SC-CO2) method. The yield of lipid extracted was calculated in comparison with the soxhlet extraction using ethyl ether as solvent. The results showed that enzyme-assisted aqueous extraction allowed recovering 82.0 ± 2.1,76.0 ± 2.3,72.9 ± 3.6, and 61.5 ± 1.6% of sea urchin gonad lipid from the sample hydrolyzed by papain, neutral protease, alkaline protease, and trypsin, respectively. Meanwhile, a lipid yield of 53.7 ± 2.6% was achieved by SC-CO2 extraction. The lipid obtained was divided into unsaponifiable (sterol) and fatty acid, and analyzed by GC-MS. Results demonstrated that the compositions of sterol and fatty acid were very similar for samples extracted by different methods. PUFA was the chief component which accounts for approximately 35% of the total fatty acid.

Original languageEnglish
Pages (from-to)737-743
Number of pages7
JournalEuropean Food Research and Technology
Volume230
Issue number5
DOIs
Publication statusPublished - 2010

Fingerprint

Strongylocentrotus
Strongylocentrotus nudus
Sea Urchins
Gonads
Echinoidea
Carbon Dioxide
Lipids
gonads
Carbon dioxide
Enzymes
carbon dioxide
Fatty acids
Polyunsaturated fatty acids
lipids
enzymes
Fatty Acids
Sterols
fatty acids
Unsaturated Fatty Acids
water

Keywords

  • Enzyme-assisted aqueous extraction
  • Fatty acid
  • Gonad
  • Sea urchin (Strongylocentrotus nudus)
  • Sterol
  • Supercritical carbon dioxide extraction

ASJC Scopus subject areas

  • Food Science
  • Chemistry(all)
  • Biochemistry
  • Biotechnology
  • Industrial and Manufacturing Engineering

Cite this

Extraction of lipid from sea urchin (Strongylocentrotus nudus) gonad by enzyme-assisted aqueous and supercritical carbon dioxide methods. / Zhu, Bei Wei; Qin, Lei; Zhou, Da Yong; Wu, Hai Tao; Wu, Jing; Yang, Jing Feng; Li, Dong Mei; Dong, Xiu Ping; Murata, Yoshiyuki.

In: European Food Research and Technology, Vol. 230, No. 5, 2010, p. 737-743.

Research output: Contribution to journalArticle

Zhu, Bei Wei ; Qin, Lei ; Zhou, Da Yong ; Wu, Hai Tao ; Wu, Jing ; Yang, Jing Feng ; Li, Dong Mei ; Dong, Xiu Ping ; Murata, Yoshiyuki. / Extraction of lipid from sea urchin (Strongylocentrotus nudus) gonad by enzyme-assisted aqueous and supercritical carbon dioxide methods. In: European Food Research and Technology. 2010 ; Vol. 230, No. 5. pp. 737-743.
@article{7a9ea7e3b8b14803a0b631593e1af861,
title = "Extraction of lipid from sea urchin (Strongylocentrotus nudus) gonad by enzyme-assisted aqueous and supercritical carbon dioxide methods",
abstract = "As rich in polyunsaturated fatty acid (PUFA), sea urchin gonad could be used as a potential resource of such type ingredients. In the present study, lipid was extracted from sea urchin (Strongylocentrotus nudus) gonad, without using organic solvent but using an enzyme-assisted aqueous method and a supercritical carbon dioxide (SC-CO2) method. The yield of lipid extracted was calculated in comparison with the soxhlet extraction using ethyl ether as solvent. The results showed that enzyme-assisted aqueous extraction allowed recovering 82.0 ± 2.1,76.0 ± 2.3,72.9 ± 3.6, and 61.5 ± 1.6{\%} of sea urchin gonad lipid from the sample hydrolyzed by papain, neutral protease, alkaline protease, and trypsin, respectively. Meanwhile, a lipid yield of 53.7 ± 2.6{\%} was achieved by SC-CO2 extraction. The lipid obtained was divided into unsaponifiable (sterol) and fatty acid, and analyzed by GC-MS. Results demonstrated that the compositions of sterol and fatty acid were very similar for samples extracted by different methods. PUFA was the chief component which accounts for approximately 35{\%} of the total fatty acid.",
keywords = "Enzyme-assisted aqueous extraction, Fatty acid, Gonad, Sea urchin (Strongylocentrotus nudus), Sterol, Supercritical carbon dioxide extraction",
author = "Zhu, {Bei Wei} and Lei Qin and Zhou, {Da Yong} and Wu, {Hai Tao} and Jing Wu and Yang, {Jing Feng} and Li, {Dong Mei} and Dong, {Xiu Ping} and Yoshiyuki Murata",
year = "2010",
doi = "10.1007/s00217-010-1216-8",
language = "English",
volume = "230",
pages = "737--743",
journal = "European Food Research and Technology",
issn = "1438-2377",
publisher = "Springer Verlag",
number = "5",

}

TY - JOUR

T1 - Extraction of lipid from sea urchin (Strongylocentrotus nudus) gonad by enzyme-assisted aqueous and supercritical carbon dioxide methods

AU - Zhu, Bei Wei

AU - Qin, Lei

AU - Zhou, Da Yong

AU - Wu, Hai Tao

AU - Wu, Jing

AU - Yang, Jing Feng

AU - Li, Dong Mei

AU - Dong, Xiu Ping

AU - Murata, Yoshiyuki

PY - 2010

Y1 - 2010

N2 - As rich in polyunsaturated fatty acid (PUFA), sea urchin gonad could be used as a potential resource of such type ingredients. In the present study, lipid was extracted from sea urchin (Strongylocentrotus nudus) gonad, without using organic solvent but using an enzyme-assisted aqueous method and a supercritical carbon dioxide (SC-CO2) method. The yield of lipid extracted was calculated in comparison with the soxhlet extraction using ethyl ether as solvent. The results showed that enzyme-assisted aqueous extraction allowed recovering 82.0 ± 2.1,76.0 ± 2.3,72.9 ± 3.6, and 61.5 ± 1.6% of sea urchin gonad lipid from the sample hydrolyzed by papain, neutral protease, alkaline protease, and trypsin, respectively. Meanwhile, a lipid yield of 53.7 ± 2.6% was achieved by SC-CO2 extraction. The lipid obtained was divided into unsaponifiable (sterol) and fatty acid, and analyzed by GC-MS. Results demonstrated that the compositions of sterol and fatty acid were very similar for samples extracted by different methods. PUFA was the chief component which accounts for approximately 35% of the total fatty acid.

AB - As rich in polyunsaturated fatty acid (PUFA), sea urchin gonad could be used as a potential resource of such type ingredients. In the present study, lipid was extracted from sea urchin (Strongylocentrotus nudus) gonad, without using organic solvent but using an enzyme-assisted aqueous method and a supercritical carbon dioxide (SC-CO2) method. The yield of lipid extracted was calculated in comparison with the soxhlet extraction using ethyl ether as solvent. The results showed that enzyme-assisted aqueous extraction allowed recovering 82.0 ± 2.1,76.0 ± 2.3,72.9 ± 3.6, and 61.5 ± 1.6% of sea urchin gonad lipid from the sample hydrolyzed by papain, neutral protease, alkaline protease, and trypsin, respectively. Meanwhile, a lipid yield of 53.7 ± 2.6% was achieved by SC-CO2 extraction. The lipid obtained was divided into unsaponifiable (sterol) and fatty acid, and analyzed by GC-MS. Results demonstrated that the compositions of sterol and fatty acid were very similar for samples extracted by different methods. PUFA was the chief component which accounts for approximately 35% of the total fatty acid.

KW - Enzyme-assisted aqueous extraction

KW - Fatty acid

KW - Gonad

KW - Sea urchin (Strongylocentrotus nudus)

KW - Sterol

KW - Supercritical carbon dioxide extraction

UR - http://www.scopus.com/inward/record.url?scp=77950534949&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950534949&partnerID=8YFLogxK

U2 - 10.1007/s00217-010-1216-8

DO - 10.1007/s00217-010-1216-8

M3 - Article

AN - SCOPUS:77950534949

VL - 230

SP - 737

EP - 743

JO - European Food Research and Technology

JF - European Food Research and Technology

SN - 1438-2377

IS - 5

ER -