Extraction of DNA by reversed micelles

Masahiro Goto, Tsutomu Ono, Akihiko Horiuchi, Shintaro Furusaki

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Deoxyribonucleotide (DNA) from salmon testes was successfully extracted by reversed micelles that are formed by a cationic surfactant in isooctane. The degree of extraction of DNA was strongly influenced by the type of surfactant in the organic solvent and ionic strength in the material aqueous phase. The driving force of the DNA transfer was electrostatic interaction between cationic surfactants and negatively charged DNA. Cationic surfactants possessing two long-alkyl chains are one of the best candidates for the successful transfer of DNA from an aqueous solution to a reversed micellar solution.

Original languageEnglish
Pages (from-to)123-125
Number of pages3
JournalJournal of Chemical Engineering of Japan
Volume32
Issue number1
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Micelles
DNA
Cationic surfactants
Deoxyribonucleotides
Coulomb interactions
Ionic strength
Surface-Active Agents
Organic solvents
Surface active agents

Keywords

  • Biotechnology
  • Molecular Assembly
  • Separation
  • Surfactant

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Goto, M., Ono, T., Horiuchi, A., & Furusaki, S. (1999). Extraction of DNA by reversed micelles. Journal of Chemical Engineering of Japan, 32(1), 123-125.

Extraction of DNA by reversed micelles. / Goto, Masahiro; Ono, Tsutomu; Horiuchi, Akihiko; Furusaki, Shintaro.

In: Journal of Chemical Engineering of Japan, Vol. 32, No. 1, 1999, p. 123-125.

Research output: Contribution to journalArticle

Goto, M, Ono, T, Horiuchi, A & Furusaki, S 1999, 'Extraction of DNA by reversed micelles', Journal of Chemical Engineering of Japan, vol. 32, no. 1, pp. 123-125.
Goto, Masahiro ; Ono, Tsutomu ; Horiuchi, Akihiko ; Furusaki, Shintaro. / Extraction of DNA by reversed micelles. In: Journal of Chemical Engineering of Japan. 1999 ; Vol. 32, No. 1. pp. 123-125.
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