Extract of Vinegar "Kurosu" from Unpolished Rice Inhibits the Proliferation of Human Cancer Cells

Kumiko Nanda, N. Miyoshi, Yoshimasa Nakamura, Y. Shimoji, Y. Tamura, Y. Nishikawa, K. Uenakai, H. Kohno, T. Tanaka

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The effects of the ethyl acetate extract of "Kurosu" (EK), Japanese traditional vinegar from unpolished rice, on the proliferation of a variety of human cancer cell lines were investigated by using the alamar blue assay. Cancer cell lines included colon adenocarcinoma (Caco-2), lung carcinoma (A549), breast adenocarcinoma (MCF-7), bladder carcinoma (5637), and prostate carcinoma (LNCaP) cells. EK inhibited the proliferation of all tested cell lines in a dose-dependent manner, with inhibition mostly pronounced in Caco-2 cells (up to 62% inhibition at a dose level of 0.025%). Flow cytometry of EK-treated Caco-2 cells showed a decrease in cell number in the G2/M phase and an increase in the sub-G1 phase (apoptotic). In addition, DNA fragmentation was detected in Caco-2 cells cultured with EK by immunostaining. RT-PCR analysis revealed p21 mRNA expression was induced in EK-treated Caco-2 cells. Moreover, PARP cleavage was promoted in EK-treated Caco-2 cells. These results suggest that EK causes G0/G1 arrest through p21 induction and, thus, is a potential apoptosis inducer in Caco-2 cells.

Original languageEnglish
Pages (from-to)69-75
Number of pages7
JournalJournal of Experimental and Clinical Cancer Research
Volume23
Issue number1
Publication statusPublished - Mar 2004
Externally publishedYes

Fingerprint

Caco-2 Cells
Acetic Acid
Neoplasms
Cell Line
Adenocarcinoma
Carcinoma
G2 Phase
G1 Phase
DNA Fragmentation
Cell Division
Oryza
Prostate
Flow Cytometry
Colon
Urinary Bladder
Cell Count
Apoptosis
Breast Neoplasms
Polymerase Chain Reaction
Lung

Keywords

  • "Kurosu"
  • Apoptosis
  • Cell cycle
  • Human cancer cell line
  • Proliferation
  • Vinegar

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Extract of Vinegar "Kurosu" from Unpolished Rice Inhibits the Proliferation of Human Cancer Cells. / Nanda, Kumiko; Miyoshi, N.; Nakamura, Yoshimasa; Shimoji, Y.; Tamura, Y.; Nishikawa, Y.; Uenakai, K.; Kohno, H.; Tanaka, T.

In: Journal of Experimental and Clinical Cancer Research, Vol. 23, No. 1, 03.2004, p. 69-75.

Research output: Contribution to journalArticle

Nanda, K, Miyoshi, N, Nakamura, Y, Shimoji, Y, Tamura, Y, Nishikawa, Y, Uenakai, K, Kohno, H & Tanaka, T 2004, 'Extract of Vinegar "Kurosu" from Unpolished Rice Inhibits the Proliferation of Human Cancer Cells', Journal of Experimental and Clinical Cancer Research, vol. 23, no. 1, pp. 69-75.
Nanda, Kumiko ; Miyoshi, N. ; Nakamura, Yoshimasa ; Shimoji, Y. ; Tamura, Y. ; Nishikawa, Y. ; Uenakai, K. ; Kohno, H. ; Tanaka, T. / Extract of Vinegar "Kurosu" from Unpolished Rice Inhibits the Proliferation of Human Cancer Cells. In: Journal of Experimental and Clinical Cancer Research. 2004 ; Vol. 23, No. 1. pp. 69-75.
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