Extracellular-regulated-kinase 5-mediated renal protection against ischemia-reperfusion injury

Tomoko Kawakami, Sang Won Park, Ryuji Kaku, Jay Yang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

ERK5, a member of the mitogen activated protein kinase, expressed in the kidneys was smaller (~80. kDa) in apparent molecular mass compared to other organs (~120. kDa). A blocking peptide experiment confirmed that the ~80. kDa detected on Western blots was a specific band detected by the anti-ERK5 antibody. Expression of the known ERK5 variants ERK5a, b, c, and T confirmed that none of the known splice variants encoded for the renal-specific ~80. kDa protein. However, RT-PCR with primers targeting the potential splice sites did not reveal a novel transcript in the kidney. The smaller molecular mass of the kidney-specific ERK5-immunoreactive protein suggested that this cyto-protective molecule may not be fully functional in the kidneys. Lentivirus-mediated in vivo overexpression of full length ERK5 in the mouse kidneys provided protection against renal IR injury. The identity of the renal-specific ~80. kDa ERK5 remains unknown but a better understanding of the ERK5 expression and post-translational processing in the kidneys may reveal a novel strategy for renal protection.

Original languageEnglish
Pages (from-to)603-608
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume418
Issue number4
DOIs
Publication statusPublished - Feb 24 2012

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Keywords

  • ERK5
  • IR injury
  • MAPK
  • Mobility shift
  • Viral transduction
  • Western blot

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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