Extensive cell death in thymocytes in colon 26-induced cachectic mice

S. Otsuka, H. Iwagaki, Tadashi Yoshino, Y. Nitta, Y. Takeuchi, M. Uomoto, A. Gouchi, Masahiro Nishibori, N. Tanaka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Extensive atrophy has been reported to occur in the thymus in a cancer-burden state but the mechanisms of this atrophy have not been fully elucidated. We investigated changes in the thymus in tumour-bearing mice inoculated with two subclones of the murine colon 26 adenocarcinoma cell line: clone 5 (non-cachectic) and clone 20 (cachectic). In clone 20 mice, body weights and thymocyte numbers decreased significantly compared with controls. Flow cytometric analysis of the thymocytes demonstrated that the frequency of single positive cells (CD4+ CD8- and CD4- CD8+) was significantly increased and that of double positive cells (CD4+ CD8+) was significantly decreased in clone 20 mice and, to a lesser extent, in clone 5 mice compared with controls. Serum levels of interleukin 6 and granulocyte-macrophage colony-stimulating factor (GM-CSF) were significantly elevated. These results suggested that thymocyte apoptosis was accelerated in the cancer-cachectic state, and increased GM-CSF might be partly responsible for thymic atrophy.

Original languageEnglish
Pages (from-to)36-45
Number of pages10
JournalJournal of International Medical Research
Volume28
Issue number1
Publication statusPublished - 2000

Fingerprint

Cell death
Thymocytes
Thymus
Colon
Cell Death
Clone Cells
Cells
Granulocyte-Macrophage Colony-Stimulating Factor
Atrophy
Bearings (structural)
Tumors
Interleukin-6
Thymus Neoplasms
Apoptosis
Thymus Gland
Neoplasms
Adenocarcinoma
Body Weight
Cell Line
Serum

Keywords

  • Apoptosis
  • Cachexia
  • Cancer
  • Thymus

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Otsuka, S., Iwagaki, H., Yoshino, T., Nitta, Y., Takeuchi, Y., Uomoto, M., ... Tanaka, N. (2000). Extensive cell death in thymocytes in colon 26-induced cachectic mice. Journal of International Medical Research, 28(1), 36-45.

Extensive cell death in thymocytes in colon 26-induced cachectic mice. / Otsuka, S.; Iwagaki, H.; Yoshino, Tadashi; Nitta, Y.; Takeuchi, Y.; Uomoto, M.; Gouchi, A.; Nishibori, Masahiro; Tanaka, N.

In: Journal of International Medical Research, Vol. 28, No. 1, 2000, p. 36-45.

Research output: Contribution to journalArticle

Otsuka, S, Iwagaki, H, Yoshino, T, Nitta, Y, Takeuchi, Y, Uomoto, M, Gouchi, A, Nishibori, M & Tanaka, N 2000, 'Extensive cell death in thymocytes in colon 26-induced cachectic mice', Journal of International Medical Research, vol. 28, no. 1, pp. 36-45.
Otsuka, S. ; Iwagaki, H. ; Yoshino, Tadashi ; Nitta, Y. ; Takeuchi, Y. ; Uomoto, M. ; Gouchi, A. ; Nishibori, Masahiro ; Tanaka, N. / Extensive cell death in thymocytes in colon 26-induced cachectic mice. In: Journal of International Medical Research. 2000 ; Vol. 28, No. 1. pp. 36-45.
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