Extending chestnut blight hypovirus host range within diaporthales by biolistic delivery of viral cDNA

Atsuko Sasaki, Mari Onoue, Satoko Kanematsu, Kouich Suzaki, Masaki Miyanishi, Nobuhiro Suzuki, Donald L. Nuss, Kouji Yoshida

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Biolistic bombardment was used to successfully transform three phytopathogenic fungal species with an infectious cDNA done of the prototypic hypovirus, CHV1-EP713, a genetic element responsible for the virulence attenuation (hypovirulence) of the chestnut blight fungus, Cryphonectria parasitica. The fungal species included two strains each of C. parasitica and Valsa ceratosperma, as well as one strain of Phomopsis G-type (teleomorph Diaporthe Nitschke); all are members of the order Diaporthales but classified into three different genera. A subset of transformants for each of the fungal species contained CHV1-EP713 dsRNA derived from chromosomally integrated viral cDNA. As has been reported for CHV1-EP713 infection of the natural host C. parasitica, biolistic introduction of CHV1-EP713 into the new fungal hosts V. ceratosperma and Phomopsis G-type resulted in altered colony morphology and, more importantly, reduced virulence. These results suggest a potential for hypoviruses as biological control agents in plant-infecting fungal pathogens other than the chestnut blight fungus and closely related species. In addition, the particle delivery technique offers a convenient means of transmitting hypoviruses to potential host fungi that provides new avenues for fundamental mycovirus research and may have practical applications for conferring hypovirulence directly on infected plants in the field.

Original languageEnglish
Pages (from-to)780-789
Number of pages10
JournalMolecular Plant-Microbe Interactions
Volume15
Issue number8
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Hypovirus
Diaporthales
Biolistics
Cryphonectria parasitica
biolistics
Host Specificity
Fungi
host range
Diaporthe
Hypoviridae
Complementary DNA
virulence
Virulence
Biological Control Agents
fungi
Pathogens
Valsa
double-stranded RNA
biological control agents
Infection

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Cite this

Sasaki, A., Onoue, M., Kanematsu, S., Suzaki, K., Miyanishi, M., Suzuki, N., ... Yoshida, K. (2002). Extending chestnut blight hypovirus host range within diaporthales by biolistic delivery of viral cDNA. Molecular Plant-Microbe Interactions, 15(8), 780-789.

Extending chestnut blight hypovirus host range within diaporthales by biolistic delivery of viral cDNA. / Sasaki, Atsuko; Onoue, Mari; Kanematsu, Satoko; Suzaki, Kouich; Miyanishi, Masaki; Suzuki, Nobuhiro; Nuss, Donald L.; Yoshida, Kouji.

In: Molecular Plant-Microbe Interactions, Vol. 15, No. 8, 2002, p. 780-789.

Research output: Contribution to journalArticle

Sasaki, A, Onoue, M, Kanematsu, S, Suzaki, K, Miyanishi, M, Suzuki, N, Nuss, DL & Yoshida, K 2002, 'Extending chestnut blight hypovirus host range within diaporthales by biolistic delivery of viral cDNA', Molecular Plant-Microbe Interactions, vol. 15, no. 8, pp. 780-789.
Sasaki, Atsuko ; Onoue, Mari ; Kanematsu, Satoko ; Suzaki, Kouich ; Miyanishi, Masaki ; Suzuki, Nobuhiro ; Nuss, Donald L. ; Yoshida, Kouji. / Extending chestnut blight hypovirus host range within diaporthales by biolistic delivery of viral cDNA. In: Molecular Plant-Microbe Interactions. 2002 ; Vol. 15, No. 8. pp. 780-789.
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