Expressions of lipoprotein receptors and cholesterol efflux regulatory proteins during luteolysis in bovine corpus luteum

Kei Horihata, Shin Yoshioka, Masahiro Sano, Yuki Yamamoto, Koji Kimura, Dariusz J. Skarzynski, Kiyoshi Okuda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The corpus luteum (CL) synthesises and secretes progesterone (P4), which is essential for the establishment and maintenance of pregnancy in mammals. P4 is synthesised from cholesterol. Cholesterol is internalised by low-density lipoprotein receptor (LDLR) and/or scavenger receptor B1 (SR-BI), and is effluxed by ATP-binding cassette (ABC) transporter A1 (ABCA1) and G1 (ABCG1). To test the hypothesis that lipoprotein receptors and ABC transporters are involved in functional luteolysis, we examined the expression of LDLR, SR-BI, ABCA1 and ABCG1 in bovine CL during the luteal stages and after injection of prostaglandin (PG) F2α on Day 10 after ovulation. Expression of LDLR and SR-BI mRNA and protein was lower in the regressed luteal than late luteal stage. Injection of cows with a PGF2α did not affect LDLR mRNA and protein levels in the CL. Although expression of SR-BI mRNA did not change, SR-BI protein expression decreased 12 and 24h after PGF2α injection. The overall findings of the present study suggest that the decreased expression of SR-BI induced by PGF2α is one of the factors responsible for the continuous decrease in P4 production during functional luteolysis.

Original languageEnglish
Pages (from-to)1280-1286
Number of pages7
JournalReproduction, Fertility and Development
Volume29
Issue number7
DOIs
Publication statusPublished - 2017

Fingerprint

ATP Binding Cassette Transporter 1
Lipoprotein Receptors
Luteolysis
luteolysis
Corpus Luteum
regulatory proteins
corpus luteum
lipoproteins
Dinoprost
LDL Receptors
cholesterol
low density lipoprotein
receptors
ABC transporters
ATP-Binding Cassette Transporters
cattle
CD36 Antigens
injection
Messenger RNA
Injections

Keywords

  • luteal phase
  • ovary
  • progesterone
  • prostaglandin
  • reproduction.

ASJC Scopus subject areas

  • Biotechnology
  • Reproductive Medicine
  • Molecular Biology
  • Animal Science and Zoology
  • Genetics
  • Endocrinology
  • Developmental Biology

Cite this

Expressions of lipoprotein receptors and cholesterol efflux regulatory proteins during luteolysis in bovine corpus luteum. / Horihata, Kei; Yoshioka, Shin; Sano, Masahiro; Yamamoto, Yuki; Kimura, Koji; Skarzynski, Dariusz J.; Okuda, Kiyoshi.

In: Reproduction, Fertility and Development, Vol. 29, No. 7, 2017, p. 1280-1286.

Research output: Contribution to journalArticle

Horihata, Kei ; Yoshioka, Shin ; Sano, Masahiro ; Yamamoto, Yuki ; Kimura, Koji ; Skarzynski, Dariusz J. ; Okuda, Kiyoshi. / Expressions of lipoprotein receptors and cholesterol efflux regulatory proteins during luteolysis in bovine corpus luteum. In: Reproduction, Fertility and Development. 2017 ; Vol. 29, No. 7. pp. 1280-1286.
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