Expressions of estrogen receptors in the bovine corpus luteum: Cyclic changes and effects of prostaglandin F2α and cytokines

Masami Shibaya, Ayumi Matsuda, Takuo Hojo, Tomas Javier Acosta, Kiyoshi Okuda

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Estrogen (E) exerts its function by binding to two intracellular estrogen receptors, ERα and ERβ. Although ERs have been reported to be expressed in the bovine corpus luteum (CL), the mechanisms that control ER expression in the bovine CL are not fully understood. To determine the possible regulatory mechanisms of ERα and ERβ that meditate distinct E functions, we examined 1) the changes in the protein expressions of ERs in the CL throughout the luteal phase and 2) the effects of prostaglandin (PG) F2α, tumor necrosis factor-α (TNFα) and interferon-γ (IFNγ) on the expressions of ERs in cultured bovine luteal cells. Western blot analyses revealed that ERα and ERβ proteins were expressed throughout the luteal phase. The ERα protein level was high at the early luteal (Days 2-3 after ovulation) and mid-luteal stages (Days 8-12) and was extremely low at the regressed luteal stage (Days 19-21). The ERβ protein level increased from the early to developing luteal stage, remained at the same level at the mid-luteal stage and decreased thereafter. The ratio of ERβ to ERα was higher in the regressed stage than in the other stages. Luteal cells obtained from mid-stage CLs (Days 8-12) were incubated with PGF2α (0.01-1 μM), TNFα (0.0145-1.45 nM) or IFNγ (0.0125-1.25 nM) for 24 h. PGF2α and TNFα inhibited ERα and ERβ mRNA expressions. IFNγ suppressed ERβ mRNA expression but did not affect the expression of ERα mRNA. However, the ERα and ERβ protein levels were not affected by any of the above treatments. These data indicate that PGF2α, TNFα and IFNγ regulate ERα and ERβ mRNA expressions in bovine luteal cells. Moreover, the changes in the ERβ/ ERα ratio throughout the luteal phase suggest that ERα is associated with luteal maintenance. Therefore, a dramatic decrease in ERα at the regressed luteal stage could result in progression of structural luteolysis in the bovine CL.

Original languageEnglish
Pages (from-to)1059-1068
Number of pages10
JournalJournal of Reproduction and Development
Volume53
Issue number5
DOIs
Publication statusPublished - Oct 2007

Fingerprint

Dinoprost
Corpus Luteum
corpus luteum
Estrogen Receptors
prostaglandins
cytokines
Cytokines
cattle
Luteal Cells
Interferons
Luteal Phase
tumor necrosis factors
Tumor Necrosis Factor-alpha
interferons
luteal cells
Messenger RNA
Proteins
Corpus Luteum Maintenance
estrogen receptors
Luteolysis

Keywords

  • Cattle
  • Corpus luteum
  • Cytokines
  • Estrogen receptor
  • Prostaglandin F2α

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine

Cite this

Expressions of estrogen receptors in the bovine corpus luteum : Cyclic changes and effects of prostaglandin F2α and cytokines. / Shibaya, Masami; Matsuda, Ayumi; Hojo, Takuo; Acosta, Tomas Javier; Okuda, Kiyoshi.

In: Journal of Reproduction and Development, Vol. 53, No. 5, 10.2007, p. 1059-1068.

Research output: Contribution to journalArticle

Shibaya, Masami ; Matsuda, Ayumi ; Hojo, Takuo ; Acosta, Tomas Javier ; Okuda, Kiyoshi. / Expressions of estrogen receptors in the bovine corpus luteum : Cyclic changes and effects of prostaglandin F2α and cytokines. In: Journal of Reproduction and Development. 2007 ; Vol. 53, No. 5. pp. 1059-1068.
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