Expression profiles and unc-27 mutation rescue of the striated muscle type troponin I isoform-3in Caenorhabditis elegans

Yasuo Takashima, Shun Kitaoka, Tetsuya Bando, Hiroaki Kagawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Transcription control of multiple genes in tissue- and stage-specific patterns is still of major interest. We show here that troponin I (TNI) is expressed under the control of upstream non-coding sequences and had functions as an isoform of intermediate type between pharynx and body-wall of the gene. In Caenorhabditis elegans, three striated muscle TNIs are expressed in body-wall muscles and a cardiac isoform is expressed in the pharynx. We have analyzed the gene expression mechanisms of tni-3 gene and motility function of its protein product. Promoter deletion analysis of the tni-3 gene identified muscle enhancers including the head enhancer. The CBF1/Su(H)/LAG-1-binding motif was included in the head enhancer. Yeast one-hybrid screening isolated the lag-1 clone in five candidates.Functional differences between the three striated muscle TNIs were investigated by the expression of promoter-fusion genes into tni-2/unc-27(e155) null mutant animals. The results suggest that the cis-elements in the promoters of the three genes are important for their tissue-specific expression and that from the function of TNI-3, the tni-3 gene would be an intermediate in the evolution of these genes by gene duplication. Mechanisms of tni-3 expression and its molecular function may contribute to our understanding of gene evolution and developmental programs.

Original languageEnglish
Pages (from-to)243-251
Number of pages9
JournalGenes and Genetic Systems
Volume87
Issue number4
Publication statusPublished - 2012

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Keywords

  • Body-wall muscle
  • Caenorhabditis elegans
  • CBF1/Su(H)/LAG-1
  • Transcriptional regulation
  • Troponin I

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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