Expression profile of vesicular nucleotide transporter (VNUT, SLC17A9) in subpopulations of rat dorsal root ganglion neurons

Kentaro Nishida, Yuka Nomura, Kanako Kawamori, Yoshinori Moriyama, Kazuki Nagasawa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

ATP plays an important role in the signal transduction between sensory neurons and satellite cells in dorsal root ganglia (DRGs). In primary cultured DRG neurons, ATP is known to be stored in lysosomes via a vesicular nucleotide transporter (VNUT), and to be released into the intercellular space through exocytosis. DRGs consist of large-, medium- and small-sized neurons, which play different roles in sensory transmission, but there is no information on the expression profiles of VNUT in DRG subpopulations. Here, we obtained detailed expression profiles of VNUT in isolated rat DRG tissues. On immunohistochemical analysis, VNUT was found in DRG neurons, and was predominantly expressed by the small- and medium-sized DRG ones, as judged upon visual inspection, and this was compatible with the finding that the number of VNUT-positive DRG neurons in IB4-positive cells was greater than that in NF200-positive ones. These results suggest that VNUT play a role in ATP accumulation in DRG neurons, especially in small- and medium-sized ones, and might be involved in ATP-mediated nociceptive signaling in DRGs.

Original languageEnglish
Pages (from-to)75-79
Number of pages5
JournalNeuroscience Letters
Volume579
DOIs
Publication statusPublished - Sep 5 2014

Fingerprint

Spinal Ganglia
Nucleotides
Neurons
Adenosine Triphosphate
Exocytosis
Extracellular Space
Sensory Receptor Cells
Lysosomes
Signal Transduction

Keywords

  • ATP
  • Dorsal root ganglion
  • SLC17A9
  • Vesicular nucleotide transporter

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Expression profile of vesicular nucleotide transporter (VNUT, SLC17A9) in subpopulations of rat dorsal root ganglion neurons. / Nishida, Kentaro; Nomura, Yuka; Kawamori, Kanako; Moriyama, Yoshinori; Nagasawa, Kazuki.

In: Neuroscience Letters, Vol. 579, 05.09.2014, p. 75-79.

Research output: Contribution to journalArticle

Nishida, Kentaro ; Nomura, Yuka ; Kawamori, Kanako ; Moriyama, Yoshinori ; Nagasawa, Kazuki. / Expression profile of vesicular nucleotide transporter (VNUT, SLC17A9) in subpopulations of rat dorsal root ganglion neurons. In: Neuroscience Letters. 2014 ; Vol. 579. pp. 75-79.
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