Expression profile of arylphorin gene during diapause and cold acclimation in the rice stem borer, Chilo suppressalis Walker (Lepidoptera: Crambidae)

Shoji Sonoda, Katsue Fukumoto, Yohei Izumi, Muhammad Ashfaq, Hideya Yoshida, Hisaaki Tsumuki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A storage protein gene, CsSP2, was cloned and sequenced from the rice stem borer, Chilo suppressalis. Analysis of the cDNA sequence revealed an open reading frame of 2,118 bp in length which encodes an arylphorin-like protein with a calculated molecular weight of 83.7 kDa. The expression level of CsSP2 was higher in non-diapausing larvae than in diapausing larvae at 20°C. When diapausing larvae were acclimatized in a stepwise fashion from 20°C to 5°C, CsSP2 expression was up-regulated five days after acclimation at 15°C. The level was maintained for 10 days after acclimation at 5°C and down-regulated thereafter. Some up-regulation in CsSP2 expression was detected in the post-diapause state. In non-diapausing larvae, CsSP2 expression was down-regulated in the course of cold acclimation. Protein products of CsSP2 might have an important role as an amino acid reservoir or a cellular defense mechanism during diapause.

Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalApplied Entomology and Zoology
Volume42
Issue number1
DOIs
Publication statusPublished - 2007

Fingerprint

arylphorins
Chilo suppressalis
Crambidae
diapause
acclimation
Lepidoptera
larvae
genes
protein products
storage proteins
defense mechanisms
open reading frames
molecular weight
amino acids
proteins

Keywords

  • Arylphorin
  • Cold acclimation
  • Diapause
  • Hexamerin
  • Rice stem borer

ASJC Scopus subject areas

  • Insect Science

Cite this

Expression profile of arylphorin gene during diapause and cold acclimation in the rice stem borer, Chilo suppressalis Walker (Lepidoptera : Crambidae). / Sonoda, Shoji; Fukumoto, Katsue; Izumi, Yohei; Ashfaq, Muhammad; Yoshida, Hideya; Tsumuki, Hisaaki.

In: Applied Entomology and Zoology, Vol. 42, No. 1, 2007, p. 35-40.

Research output: Contribution to journalArticle

Sonoda, Shoji ; Fukumoto, Katsue ; Izumi, Yohei ; Ashfaq, Muhammad ; Yoshida, Hideya ; Tsumuki, Hisaaki. / Expression profile of arylphorin gene during diapause and cold acclimation in the rice stem borer, Chilo suppressalis Walker (Lepidoptera : Crambidae). In: Applied Entomology and Zoology. 2007 ; Vol. 42, No. 1. pp. 35-40.
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