Expression patterns of the opsin 5-related genes in the developing chicken retina

Sayuri Tomonari, Kyoichi Migita, Akira Takagi, Sumihare Noji, Hideyo Ohuchi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The opsin gene family encodes G protein-coupled seven-transmembrane proteins that bind to a retinaldehyde chromophore for photoreception. It has been reported that opsin 5 is expressed in mammalian neural tissue, but its function has been elusive. As a first step to understand the function for opsin 5 in the developing eye, we searched for chicken opsin 5-related genes in the genome by a bioinformatic approach and isolated opsin 5 cDNA fragments from the embryonic retina by RT-PCR. We found that there are three opsin 5-related genes, designated cOpn5m (chicken opsin 5, mammalian type), cOpn5L1 (chicken opsin 5-like 1), and cOpn5L2 (chicken opsin 5-like 2), in the chicken genome. Quantitative PCR analysis has revealed that cOpn5m is the most abundant in the developing and early posthatching neural retina. In situ hybridization analysis has shown that cOpn5m is specifically expressed in subsets of differentiating ganglion cells and amacrine cells. These results suggest that the mammalian type opsin 5 may contribute to the development of these retinal cells in the chicken.

Original languageEnglish
Pages (from-to)1910-1922
Number of pages13
JournalDevelopmental Dynamics
Volume237
Issue number7
DOIs
Publication statusPublished - Jul 2008
Externally publishedYes

Fingerprint

Opsins
Retina
Chickens
Genes
Genome
Retinaldehyde
Amacrine Cells
Polymerase Chain Reaction
Computational Biology
GTP-Binding Proteins
Ganglia
In Situ Hybridization

Keywords

  • Amacrine cells
  • Chicken
  • Development
  • Ganglion cells
  • Opsin 5
  • Retina

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Expression patterns of the opsin 5-related genes in the developing chicken retina. / Tomonari, Sayuri; Migita, Kyoichi; Takagi, Akira; Noji, Sumihare; Ohuchi, Hideyo.

In: Developmental Dynamics, Vol. 237, No. 7, 07.2008, p. 1910-1922.

Research output: Contribution to journalArticle

Tomonari, Sayuri ; Migita, Kyoichi ; Takagi, Akira ; Noji, Sumihare ; Ohuchi, Hideyo. / Expression patterns of the opsin 5-related genes in the developing chicken retina. In: Developmental Dynamics. 2008 ; Vol. 237, No. 7. pp. 1910-1922.
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