Expression patterns of the lysophospholipid receptor genes during mouse early development

Hideyo Ohuchi, Aska Hamada, Hironao Matsuda, Akira Takagi, Masayuki Tanaka, Junken Aoki, Hiroyuki Arai, Sumihare Noji

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55 Citations (Scopus)

Abstract

Lysophospholipids (LPs) such as lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are known to mediate various biological responses, including cell proliferation, migration, and differentiation. To better understand the role of these lipids in mammalian early development, we applied whole-mount in situ hybridization techniques to E8.5 to E12.5 mouse embryos. We determined the expression patterns of the following LP receptor genes, which belong to the G protein-coupled receptor (GPCR) family: EDG1 to EDG8 (S1P 1 to S1P5 and LPA1 to LPA3), LPA4 (GPR23 /P2Y9), and LPA5 (GPR92). We found that the S1P/LPA receptor genes exhibit overlapping expression patterns in a variety of organ primordia, including the developing brain and cardiovascular system, presomitic mesoderm and somites, branchial arches, and limb buds. These results suggest that multiple receptor systems for LPA/S1P lysophospholipids may be functioning during organogenesis.

Original languageEnglish
Pages (from-to)3280-3294
Number of pages15
JournalDevelopmental Dynamics
Volume237
Issue number11
DOIs
Publication statusPublished - Nov 1 2008
Externally publishedYes

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Keywords

  • Edg
  • Endothelial differentiation genes
  • G protein-coupled receptors
  • GPR23
  • GPR92
  • Gna13
  • LPA
  • Lysophosphatidic acid
  • S1P
  • Sphingosine-1-phosphate

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Ohuchi, H., Hamada, A., Matsuda, H., Takagi, A., Tanaka, M., Aoki, J., Arai, H., & Noji, S. (2008). Expression patterns of the lysophospholipid receptor genes during mouse early development. Developmental Dynamics, 237(11), 3280-3294. https://doi.org/10.1002/dvdy.21736