Expression patterns in alternative splicing forms of prosaposin mRNA in the rat facial nerve nucleus after facial nerve transection

Jie Chen, Shouichiro Saito, Naoto Kobayashi, Kohji Sato, Takehiro Terashita, Tetsuya Shimokawa, Katsumi Mominoki, Kyojy Miyawaki, Akira Sano, Seiji Matsuda

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18 Citations (Scopus)

Abstract

Prosaposin acts as a neurotrophic factor, in addition to its role as the precursor protein for saposins A, B, C, and D, which are activators for specific sphingolipid hydrolases in lysosomes. In rats, the prosaposin gene generates two alternative splicing forms of mRNA: Pro + 9 containing a 9-base insertion and Pro + 0 without. The expression of these mRNAs changes after brain injury. We examined the expression patterns of the alternative splicing forms of prosaposin mRNA in the rat facial nerve nucleus for 52 days following facial nerve transection. Pro + 0 mRNA increased within 3 days of transection, peaked after 5-10 days, and remained significantly elevated for 21 days. In contrast, the expression of Pro + 9 mRNA was constant throughout the regenerative period. Prosaposin mRNA expression increased not only in facial motoneurons, but also in microglia during facial nerve regeneration. Our findings indicate that the saposin B domain of prosaposin, which is the domain affected by alternative splicing, plays an important role in both neurons and microglia during neuroregeneration.

Original languageEnglish
Pages (from-to)82-94
Number of pages13
JournalNeuroscience Research
Volume60
Issue number1
DOIs
Publication statusPublished - Jan 1 2008

Keywords

  • Facial nerve nucleus
  • In situ hybridization
  • Microglia
  • Neuroregeneration
  • Neurotrophic factor
  • Saposin B

ASJC Scopus subject areas

  • Neuroscience(all)

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    Chen, J., Saito, S., Kobayashi, N., Sato, K., Terashita, T., Shimokawa, T., Mominoki, K., Miyawaki, K., Sano, A., & Matsuda, S. (2008). Expression patterns in alternative splicing forms of prosaposin mRNA in the rat facial nerve nucleus after facial nerve transection. Neuroscience Research, 60(1), 82-94. https://doi.org/10.1016/j.neures.2007.09.010