Expression patterns and ectopic expressions of fibroblast growth factor genes (Fgf4, Fgf8) during early chick eye development

Research output: Contribution to journalArticle

Abstract

We used whole-mount in situ hybridization to examine expression patterns of Fgf4 and Fgf8 genes during chick eye development, and found that Fgf8 is expressed in the inner and outer layers of the early optic cup, while Fgf4 transcripts were not detectable in the developing ocular tissues examined. Furthermore, to learn Fgf gene functions during chick eye development, the genes were ectopically expressed in the developing chick eye using a retrovirus. The outer layer of the optic cup overexpressing either Fgf gene became depigmented and thickened like a second neural retina. These results suggest that FGF 8 is an endogenous factor involved in neural retinal differentiation.

Original languageEnglish
Pages (from-to)1072-1076
Number of pages5
JournalFolia Ophthalmologica Japonica
Volume48
Issue number9
Publication statusPublished - Sep 1997
Externally publishedYes

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Fibroblast Growth Factors
Genes
Retroviridae
In Situ Hybridization
Retina
Ectopic Gene Expression

Keywords

  • Chick Embryo
  • Fgf4
  • Fgf8
  • Neural Retina
  • Retinal Pigment Epithelium

ASJC Scopus subject areas

  • Ophthalmology

Cite this

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abstract = "We used whole-mount in situ hybridization to examine expression patterns of Fgf4 and Fgf8 genes during chick eye development, and found that Fgf8 is expressed in the inner and outer layers of the early optic cup, while Fgf4 transcripts were not detectable in the developing ocular tissues examined. Furthermore, to learn Fgf gene functions during chick eye development, the genes were ectopically expressed in the developing chick eye using a retrovirus. The outer layer of the optic cup overexpressing either Fgf gene became depigmented and thickened like a second neural retina. These results suggest that FGF 8 is an endogenous factor involved in neural retinal differentiation.",
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AB - We used whole-mount in situ hybridization to examine expression patterns of Fgf4 and Fgf8 genes during chick eye development, and found that Fgf8 is expressed in the inner and outer layers of the early optic cup, while Fgf4 transcripts were not detectable in the developing ocular tissues examined. Furthermore, to learn Fgf gene functions during chick eye development, the genes were ectopically expressed in the developing chick eye using a retrovirus. The outer layer of the optic cup overexpressing either Fgf gene became depigmented and thickened like a second neural retina. These results suggest that FGF 8 is an endogenous factor involved in neural retinal differentiation.

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