TY - JOUR
T1 - Expression pattern of sonic hedgehog signaling and calcitonin gene-related peptide in the socket healing process after tooth extraction
AU - Pang, Pai
AU - Shimo, Tsuyoshi
AU - Takada, Hiroyuki
AU - Matsumoto, Kenichi
AU - Yoshioka, Norie
AU - Ibaragi, Soichiro
AU - Sasaki, Akira
N1 - Funding Information:
This work was partly supported by a Grant-in-Aid for scientific Research (B) (to T.S.) from the Ministry of Education, Culture, Sports, Science, and Technology ( 26293428 ) of Japan.
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/11/6
Y1 - 2015/11/6
N2 - Sonic Hedgehog (SHH), a neural development inducer, plays a significant role in the bone healing process. Calcitonin gene-related peptide (CGRP), a neuropeptide marker of sensory nerves, has been demonstrated to affect bone formation. The roles of SHH signaling and CGRP-positive sensory nerves in the alveolar bone formation process have been unknown. Here we examined the expression patterns of SHH signaling and CGRP in mouse socket by immunohistochemistry and immunofluorescence analysis. We found that the expression level of SHH peaked at day 3 and was then decreased at 5 days after tooth extraction. CGRP, PTCH1 and GLI2 were each expressed in a similar pattern with their highest expression levels at day 5 and day 7 after tooth extraction. CGRP and GLI2 were co-expressed in some inflammatory cells and bone forming cells. In some areas, CGRP-positive neurons expressed GLI2. In conclusion, SHH may affect alveolar bone healing by interacting with CGRP-positive sensory neurons and thus regulate the socket's healing process after tooth extraction.
AB - Sonic Hedgehog (SHH), a neural development inducer, plays a significant role in the bone healing process. Calcitonin gene-related peptide (CGRP), a neuropeptide marker of sensory nerves, has been demonstrated to affect bone formation. The roles of SHH signaling and CGRP-positive sensory nerves in the alveolar bone formation process have been unknown. Here we examined the expression patterns of SHH signaling and CGRP in mouse socket by immunohistochemistry and immunofluorescence analysis. We found that the expression level of SHH peaked at day 3 and was then decreased at 5 days after tooth extraction. CGRP, PTCH1 and GLI2 were each expressed in a similar pattern with their highest expression levels at day 5 and day 7 after tooth extraction. CGRP and GLI2 were co-expressed in some inflammatory cells and bone forming cells. In some areas, CGRP-positive neurons expressed GLI2. In conclusion, SHH may affect alveolar bone healing by interacting with CGRP-positive sensory neurons and thus regulate the socket's healing process after tooth extraction.
KW - Calcitonin gene-related peptide
KW - Sonic hedgehog
KW - Tooth extraction
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U2 - 10.1016/j.bbrc.2015.09.139
DO - 10.1016/j.bbrc.2015.09.139
M3 - Article
C2 - 26427874
AN - SCOPUS:84944876206
SN - 0006-291X
VL - 467
SP - 21
EP - 26
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -