Expression pattern of sonic hedgehog signaling and calcitonin gene-related peptide in the socket healing process after tooth extraction

Pai Pang, Tsuyoshi Shimo, Hiroyuki Takada, Kenichi Matsumoto, Norie Yoshioka, Soichiro Ibaragi, Akira Sasaki

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Sonic Hedgehog (SHH), a neural development inducer, plays a significant role in the bone healing process. Calcitonin gene-related peptide (CGRP), a neuropeptide marker of sensory nerves, has been demonstrated to affect bone formation. The roles of SHH signaling and CGRP-positive sensory nerves in the alveolar bone formation process have been unknown. Here we examined the expression patterns of SHH signaling and CGRP in mouse socket by immunohistochemistry and immunofluorescence analysis. We found that the expression level of SHH peaked at day 3 and was then decreased at 5 days after tooth extraction. CGRP, PTCH1 and GLI2 were each expressed in a similar pattern with their highest expression levels at day 5 and day 7 after tooth extraction. CGRP and GLI2 were co-expressed in some inflammatory cells and bone forming cells. In some areas, CGRP-positive neurons expressed GLI2. In conclusion, SHH may affect alveolar bone healing by interacting with CGRP-positive sensory neurons and thus regulate the socket's healing process after tooth extraction.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume467
Issue number1
DOIs
Publication statusPublished - Nov 6 2015

Fingerprint

Tooth Extraction
Hedgehogs
Calcitonin Gene-Related Peptide
Bone
Osteogenesis
Bone and Bones
Neurons
Sensory Receptor Cells
Neuropeptides
Fluorescent Antibody Technique
Immunohistochemistry

Keywords

  • Calcitonin gene-related peptide
  • Sonic hedgehog
  • Tooth extraction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Expression pattern of sonic hedgehog signaling and calcitonin gene-related peptide in the socket healing process after tooth extraction. / Pang, Pai; Shimo, Tsuyoshi; Takada, Hiroyuki; Matsumoto, Kenichi; Yoshioka, Norie; Ibaragi, Soichiro; Sasaki, Akira.

In: Biochemical and Biophysical Research Communications, Vol. 467, No. 1, 06.11.2015, p. 21-26.

Research output: Contribution to journalArticle

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AU - Yoshioka, Norie

AU - Ibaragi, Soichiro

AU - Sasaki, Akira

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