Expression pattern of cisplatin-induced metallothionein isoforms in squamous cell carcinoma

Makoto Nakano; Chiharu Aoki Sogawa; Norio Sogawa; Katsuaki Mishima; Eiki Yamachika; Nobuyoshi Mizukawa; Joji Fukunaga; Tomoaki Kawamoto; Koichi Sawaki; Toshio Sugahara; Hiroaki Furuta

Expression pattern of cisplatin-induced metallothionein isoforms in squamous cell carcinoma

Makoto Nakano, Chiharu Aoki Sogawa, Norio Sogawa, Katsuaki Mishima, Eiki Yamachika, Nobuyoshi Mizukawa, Joji Fukunaga, Tomoaki Kawamoto, Koichi Sawaki, Toshio Sugahara, Hiroaki Furuta

University Hospital

Research output: Contribution to journal › Article

8 Citations (Scopus)

Abstract

Cisplatin (CDDP) is a useful drug for the treatment of malignant solid tumors of the head and neck. Because CDDP includes the heavy metal platinum as a component, it is thought metallothionein (MT) may be involved in CDDP-resistance. However, functional differences between the four MT isoforms (MT-I, II, III and IV) remain unclear. The aim of this study was to investigate the relationship between MT isoform expression and CDDP-resistance. Two human tongue squamous cell carcinoma cell lines not exposed to anticancer chemotherapy were studied. The cell lines were subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) analysis before and after CDDP-treatment. Both cell lines expressed MT-I/II and MT-IV isoforms but not the MT-III isoform. Following CDDP treatment, MT-I/II mRNA levels were induced only in the CDDP-resistant cell line. Our results showed that expression of the MT I/II isoform was induced by CDDP treatment, and may play an important role in CDDP-resistance in squamous cell carcinoma of the human tongue.

Original language	English
Pages (from-to)	299-303
Number of pages	5
Journal	Anticancer research
Volume	23
Issue number	1 A
Publication status	Published - Jan 2003

Keywords

Cell line
Experimental study
Human
In vitro
Metallothionein isoforms
Squamous cell carcinoma
Tongue

ASJC Scopus subject areas

Oncology
Cancer Research

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Expression pattern of cisplatin-induced metallothionein isoforms in squamous cell carcinoma. / Nakano, Makoto; Sogawa, Chiharu Aoki; Sogawa, Norio; Mishima, Katsuaki; Yamachika, Eiki; Mizukawa, Nobuyoshi; Fukunaga, Joji; Kawamoto, Tomoaki; Sawaki, Koichi; Sugahara, Toshio; Furuta, Hiroaki.

In: Anticancer research, Vol. 23, No. 1 A, 01.2003, p. 299-303.

Research output: Contribution to journal › Article

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abstract = "Cisplatin (CDDP) is a useful drug for the treatment of malignant solid tumors of the head and neck. Because CDDP includes the heavy metal platinum as a component, it is thought metallothionein (MT) may be involved in CDDP-resistance. However, functional differences between the four MT isoforms (MT-I, II, III and IV) remain unclear. The aim of this study was to investigate the relationship between MT isoform expression and CDDP-resistance. Two human tongue squamous cell carcinoma cell lines not exposed to anticancer chemotherapy were studied. The cell lines were subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) analysis before and after CDDP-treatment. Both cell lines expressed MT-I/II and MT-IV isoforms but not the MT-III isoform. Following CDDP treatment, MT-I/II mRNA levels were induced only in the CDDP-resistant cell line. Our results showed that expression of the MT I/II isoform was induced by CDDP treatment, and may play an important role in CDDP-resistance in squamous cell carcinoma of the human tongue.",

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AU - Yamachika, Eiki

AU - Mizukawa, Nobuyoshi

AU - Fukunaga, Joji

AU - Kawamoto, Tomoaki

AU - Sawaki, Koichi

AU - Sugahara, Toshio

AU - Furuta, Hiroaki

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