Abstract
Cisplatin (CDDP) is a useful drug for the treatment of malignant solid tumors of the head and neck. Because CDDP includes the heavy metal platinum as a component, it is thought metallothionein (MT) may be involved in CDDP-resistance. However, functional differences between the four MT isoforms (MT-I, II, III and IV) remain unclear. The aim of this study was to investigate the relationship between MT isoform expression and CDDP-resistance. Two human tongue squamous cell carcinoma cell lines not exposed to anticancer chemotherapy were studied. The cell lines were subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) analysis before and after CDDP-treatment. Both cell lines expressed MT-I/II and MT-IV isoforms but not the MT-III isoform. Following CDDP treatment, MT-I/II mRNA levels were induced only in the CDDP-resistant cell line. Our results showed that expression of the MT I/II isoform was induced by CDDP treatment, and may play an important role in CDDP-resistance in squamous cell carcinoma of the human tongue.
| Original language | English |
|---|---|
| Pages (from-to) | 299-303 |
| Number of pages | 5 |
| Journal | Anticancer Research |
| Volume | 23 |
| Issue number | 1 A |
| Publication status | Published - Jan 2003 |
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Keywords
- Cell line
- Experimental study
- Human
- In vitro
- Metallothionein isoforms
- Squamous cell carcinoma
- Tongue
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Expression pattern of cisplatin-induced metallothionein isoforms in squamous cell carcinoma. / Nakano, Makoto; Sogawa, Chiharu; Sogawa, Norio; Mishima, Katsuaki; Yamachika, Eiki; Mizukawa, Nobuyoshi; Fukunaga, Joji; Kawamoto, Tomoaki; Sawaki, Koichi; Sugahara, Toshio; Furuta, Hiroaki.
In: Anticancer Research, Vol. 23, No. 1 A, 01.2003, p. 299-303.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression pattern of cisplatin-induced metallothionein isoforms in squamous cell carcinoma
AU - Nakano, Makoto
AU - Sogawa, Chiharu
AU - Sogawa, Norio
AU - Mishima, Katsuaki
AU - Yamachika, Eiki
AU - Mizukawa, Nobuyoshi
AU - Fukunaga, Joji
AU - Kawamoto, Tomoaki
AU - Sawaki, Koichi
AU - Sugahara, Toshio
AU - Furuta, Hiroaki
PY - 2003/1
Y1 - 2003/1
N2 - Cisplatin (CDDP) is a useful drug for the treatment of malignant solid tumors of the head and neck. Because CDDP includes the heavy metal platinum as a component, it is thought metallothionein (MT) may be involved in CDDP-resistance. However, functional differences between the four MT isoforms (MT-I, II, III and IV) remain unclear. The aim of this study was to investigate the relationship between MT isoform expression and CDDP-resistance. Two human tongue squamous cell carcinoma cell lines not exposed to anticancer chemotherapy were studied. The cell lines were subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) analysis before and after CDDP-treatment. Both cell lines expressed MT-I/II and MT-IV isoforms but not the MT-III isoform. Following CDDP treatment, MT-I/II mRNA levels were induced only in the CDDP-resistant cell line. Our results showed that expression of the MT I/II isoform was induced by CDDP treatment, and may play an important role in CDDP-resistance in squamous cell carcinoma of the human tongue.
AB - Cisplatin (CDDP) is a useful drug for the treatment of malignant solid tumors of the head and neck. Because CDDP includes the heavy metal platinum as a component, it is thought metallothionein (MT) may be involved in CDDP-resistance. However, functional differences between the four MT isoforms (MT-I, II, III and IV) remain unclear. The aim of this study was to investigate the relationship between MT isoform expression and CDDP-resistance. Two human tongue squamous cell carcinoma cell lines not exposed to anticancer chemotherapy were studied. The cell lines were subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) analysis before and after CDDP-treatment. Both cell lines expressed MT-I/II and MT-IV isoforms but not the MT-III isoform. Following CDDP treatment, MT-I/II mRNA levels were induced only in the CDDP-resistant cell line. Our results showed that expression of the MT I/II isoform was induced by CDDP treatment, and may play an important role in CDDP-resistance in squamous cell carcinoma of the human tongue.
KW - Cell line
KW - Experimental study
KW - Human
KW - In vitro
KW - Metallothionein isoforms
KW - Squamous cell carcinoma
KW - Tongue
UR - http://www.scopus.com/inward/record.url?scp=0344405741&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0344405741&partnerID=8YFLogxK
M3 - Article
C2 - 12680227
AN - SCOPUS:0344405741
VL - 23
SP - 299
EP - 303
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 1 A
ER -