Expression of type XVII collagen α1 chain mRNA in the mouse heart

Jun Kondo, Shozo Kusachi, Yoshifumi Ninomiya, Hidekatsu Yoshioka, Toshitaka Oohashi, Masayuki Doi, Takashi Murakami, Hiroki Moritani, Hirofumi Kumashiro, Takao Tsuji

Research output: Contribution to journalArticle

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Abstract

The type XVII collagen α1 chain has been identified as a component of the type I hemidesmosome, and is thus thought to play a role in extracellular matrix (ECM) maintenance and signal transduction between the cell and the ECM. We examined the expression of type XVII collagen α1 chain mRNA in the mouse heart by Northern blot analysis and determined the sequential changes of its expression in different developmental stages of the heart using the reverse transcriptase-polymerase chain reaction (RT-PCR) method. Northern blotting: Total RNA was extracted from 10 adult mouse hearts by the guanidine/cesium method. Hybridization was performed with mouse cDNA for α1 (XVII) collagen. RT-PCR: Total RNA was extracted from 7 embryos, 4 neonates and 8 adult mice. Reverse transcription was performed using oligo-dT primer and MMLV. Amplification was carried out in α1 (XVII) collagen and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). GAPDH served as an internal control. Northern blotting revealed a 5.6 kb signal that was identical to that of the α1 (XVII) of skin and transformed keratinocyte reported previously. The sequences of the PCR products were also identical to those reported. The normalized expression ratios of α1 (XVII) were 0.91 ± 0.20 in the embryonic heart, 0.36 ± 0.20 in the neonatal heart and 0.96 ± 0.21 in the adult heart. In conclusion, we identified the expression of type XVII collagen α1 chain mRNA in the mouse heart, suggesting that the type I hemidesmosome is located in the heart. The results of the RT-PCR at different developmental stages of the heart suggest that type XVII collagen contributes not only to cardiogenesis in the embryonic stage but also to maintenance of architecture and function in the adult heart.

Original languageEnglish
Pages (from-to)211-220
Number of pages10
JournalJapanese Heart Journal
Volume39
Issue number2
Publication statusPublished - 1998

Fingerprint

Collagen Type I
Messenger RNA
Reverse Transcriptase Polymerase Chain Reaction
Hemidesmosomes
Northern Blotting
Glyceraldehyde-3-Phosphate Dehydrogenases
Extracellular Matrix
Maintenance
RNA
Cesium
Guanidine
Keratinocytes
Reverse Transcription
Signal Transduction
Embryonic Structures
Complementary DNA
Polymerase Chain Reaction
Skin

Keywords

  • Basement membrane
  • Extracellular matrix
  • Molecular biology
  • Myocardium
  • Reverse transcriptase-polymerase chain reaction

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Kondo, J., Kusachi, S., Ninomiya, Y., Yoshioka, H., Oohashi, T., Doi, M., ... Tsuji, T. (1998). Expression of type XVII collagen α1 chain mRNA in the mouse heart. Japanese Heart Journal, 39(2), 211-220.

Expression of type XVII collagen α1 chain mRNA in the mouse heart. / Kondo, Jun; Kusachi, Shozo; Ninomiya, Yoshifumi; Yoshioka, Hidekatsu; Oohashi, Toshitaka; Doi, Masayuki; Murakami, Takashi; Moritani, Hiroki; Kumashiro, Hirofumi; Tsuji, Takao.

In: Japanese Heart Journal, Vol. 39, No. 2, 1998, p. 211-220.

Research output: Contribution to journalArticle

Kondo, J, Kusachi, S, Ninomiya, Y, Yoshioka, H, Oohashi, T, Doi, M, Murakami, T, Moritani, H, Kumashiro, H & Tsuji, T 1998, 'Expression of type XVII collagen α1 chain mRNA in the mouse heart', Japanese Heart Journal, vol. 39, no. 2, pp. 211-220.
Kondo J, Kusachi S, Ninomiya Y, Yoshioka H, Oohashi T, Doi M et al. Expression of type XVII collagen α1 chain mRNA in the mouse heart. Japanese Heart Journal. 1998;39(2):211-220.
Kondo, Jun ; Kusachi, Shozo ; Ninomiya, Yoshifumi ; Yoshioka, Hidekatsu ; Oohashi, Toshitaka ; Doi, Masayuki ; Murakami, Takashi ; Moritani, Hiroki ; Kumashiro, Hirofumi ; Tsuji, Takao. / Expression of type XVII collagen α1 chain mRNA in the mouse heart. In: Japanese Heart Journal. 1998 ; Vol. 39, No. 2. pp. 211-220.
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