TY - JOUR
T1 - Expression of two methionine-rich storage protein genes of Plutella xylostella (L.) in response to development, juvenile hormone-analog and pyrethroid
AU - Ashfaq, Muhammad
AU - Sonoda, Shoji
AU - Tsumuki, Hisaaki
N1 - Funding Information:
This study was supported in part by a Grant-in-Aid for Scientific Research (C) from the Japan Society for the Promotion of Science (JSPS) (No. 19580055) and the Ohara Foundation. M. Ashfaq thanks JSPS for the award of Postdoctoral Fellowship for Foreign Researchers.
PY - 2007/9
Y1 - 2007/9
N2 - We cloned and characterized cDNA of two storage protein (SP) genes, PxSP1 and PxSP2, from the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae) and investigated their expression. PxSP1 and PxSP2 each encoded a putative protein of 91 kDa. Nucleotide and deduced amino acid identities between the two genes were 79% and 82%, respectively. Amino acid composition (methionine > 4%), sequence homology with other insect storage proteins and the phylogenetic analysis suggested that the genes belong to the subfamily of moderately methionine-rich SP genes. The genes were predominantly expressed in the last instar female larvae and the mRNA levels were suppressed by treatment with a juvenile hormone-analog. Treatment of female larvae with sublethal dose of a pyrethroid caused a significant increase in mRNA levels of both genes. Induction of PxSP1 and PxSP2 genes as a result of pyrethroid application may have implications with respect to reproduction as methionine-rich proteins are known as a key element for egg production.
AB - We cloned and characterized cDNA of two storage protein (SP) genes, PxSP1 and PxSP2, from the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae) and investigated their expression. PxSP1 and PxSP2 each encoded a putative protein of 91 kDa. Nucleotide and deduced amino acid identities between the two genes were 79% and 82%, respectively. Amino acid composition (methionine > 4%), sequence homology with other insect storage proteins and the phylogenetic analysis suggested that the genes belong to the subfamily of moderately methionine-rich SP genes. The genes were predominantly expressed in the last instar female larvae and the mRNA levels were suppressed by treatment with a juvenile hormone-analog. Treatment of female larvae with sublethal dose of a pyrethroid caused a significant increase in mRNA levels of both genes. Induction of PxSP1 and PxSP2 genes as a result of pyrethroid application may have implications with respect to reproduction as methionine-rich proteins are known as a key element for egg production.
KW - Gene expression
KW - Juvenile hormone
KW - Methionine-rich storage protein
KW - Plutella xylostella
KW - Pyrethroid
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U2 - 10.1016/j.cbpb.2007.04.017
DO - 10.1016/j.cbpb.2007.04.017
M3 - Article
C2 - 17561427
AN - SCOPUS:34447643570
VL - 148
SP - 84
EP - 92
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
SN - 0305-0491
IS - 1
ER -