Expression of two methionine-rich storage protein genes of Plutella xylostella (L.) in response to development, juvenile hormone-analog and pyrethroid

Muhammad Ashfaq, Shoji Sonoda, Hisaaki Tsumuki

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    We cloned and characterized cDNA of two storage protein (SP) genes, PxSP1 and PxSP2, from the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae) and investigated their expression. PxSP1 and PxSP2 each encoded a putative protein of 91 kDa. Nucleotide and deduced amino acid identities between the two genes were 79% and 82%, respectively. Amino acid composition (methionine > 4%), sequence homology with other insect storage proteins and the phylogenetic analysis suggested that the genes belong to the subfamily of moderately methionine-rich SP genes. The genes were predominantly expressed in the last instar female larvae and the mRNA levels were suppressed by treatment with a juvenile hormone-analog. Treatment of female larvae with sublethal dose of a pyrethroid caused a significant increase in mRNA levels of both genes. Induction of PxSP1 and PxSP2 genes as a result of pyrethroid application may have implications with respect to reproduction as methionine-rich proteins are known as a key element for egg production.

    Original languageEnglish
    Pages (from-to)84-92
    Number of pages9
    JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
    Volume148
    Issue number1
    DOIs
    Publication statusPublished - Sep 2007

    Keywords

    • Gene expression
    • Juvenile hormone
    • Methionine-rich storage protein
    • Plutella xylostella
    • Pyrethroid

    ASJC Scopus subject areas

    • Biochemistry
    • Physiology
    • Molecular Biology

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