Abstract
We cloned and characterized cDNA of two storage protein (SP) genes, PxSP1 and PxSP2, from the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae) and investigated their expression. PxSP1 and PxSP2 each encoded a putative protein of 91 kDa. Nucleotide and deduced amino acid identities between the two genes were 79% and 82%, respectively. Amino acid composition (methionine > 4%), sequence homology with other insect storage proteins and the phylogenetic analysis suggested that the genes belong to the subfamily of moderately methionine-rich SP genes. The genes were predominantly expressed in the last instar female larvae and the mRNA levels were suppressed by treatment with a juvenile hormone-analog. Treatment of female larvae with sublethal dose of a pyrethroid caused a significant increase in mRNA levels of both genes. Induction of PxSP1 and PxSP2 genes as a result of pyrethroid application may have implications with respect to reproduction as methionine-rich proteins are known as a key element for egg production.
| Original language | English |
|---|---|
| Pages (from-to) | 84-92 |
| Number of pages | 9 |
| Journal | Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology |
| Volume | 148 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Sep 2007 |
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Keywords
- Gene expression
- Juvenile hormone
- Methionine-rich storage protein
- Plutella xylostella
- Pyrethroid
ASJC Scopus subject areas
- Biochemistry
- Physiology
Cite this
Expression of two methionine-rich storage protein genes of Plutella xylostella (L.) in response to development, juvenile hormone-analog and pyrethroid. / Ashfaq, Muhammad; Sonoda, Shoji; Tsumuki, Hisaaki.
In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 148, No. 1, 09.2007, p. 84-92.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of two methionine-rich storage protein genes of Plutella xylostella (L.) in response to development, juvenile hormone-analog and pyrethroid
AU - Ashfaq, Muhammad
AU - Sonoda, Shoji
AU - Tsumuki, Hisaaki
PY - 2007/9
Y1 - 2007/9
N2 - We cloned and characterized cDNA of two storage protein (SP) genes, PxSP1 and PxSP2, from the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae) and investigated their expression. PxSP1 and PxSP2 each encoded a putative protein of 91 kDa. Nucleotide and deduced amino acid identities between the two genes were 79% and 82%, respectively. Amino acid composition (methionine > 4%), sequence homology with other insect storage proteins and the phylogenetic analysis suggested that the genes belong to the subfamily of moderately methionine-rich SP genes. The genes were predominantly expressed in the last instar female larvae and the mRNA levels were suppressed by treatment with a juvenile hormone-analog. Treatment of female larvae with sublethal dose of a pyrethroid caused a significant increase in mRNA levels of both genes. Induction of PxSP1 and PxSP2 genes as a result of pyrethroid application may have implications with respect to reproduction as methionine-rich proteins are known as a key element for egg production.
AB - We cloned and characterized cDNA of two storage protein (SP) genes, PxSP1 and PxSP2, from the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae) and investigated their expression. PxSP1 and PxSP2 each encoded a putative protein of 91 kDa. Nucleotide and deduced amino acid identities between the two genes were 79% and 82%, respectively. Amino acid composition (methionine > 4%), sequence homology with other insect storage proteins and the phylogenetic analysis suggested that the genes belong to the subfamily of moderately methionine-rich SP genes. The genes were predominantly expressed in the last instar female larvae and the mRNA levels were suppressed by treatment with a juvenile hormone-analog. Treatment of female larvae with sublethal dose of a pyrethroid caused a significant increase in mRNA levels of both genes. Induction of PxSP1 and PxSP2 genes as a result of pyrethroid application may have implications with respect to reproduction as methionine-rich proteins are known as a key element for egg production.
KW - Gene expression
KW - Juvenile hormone
KW - Methionine-rich storage protein
KW - Plutella xylostella
KW - Pyrethroid
UR - http://www.scopus.com/inward/record.url?scp=34447643570&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34447643570&partnerID=8YFLogxK
U2 - 10.1016/j.cbpb.2007.04.017
DO - 10.1016/j.cbpb.2007.04.017
M3 - Article
C2 - 17561427
AN - SCOPUS:34447643570
VL - 148
SP - 84
EP - 92
JO - Comparative biochemistry and physiology. B, Comparative biochemistry
JF - Comparative biochemistry and physiology. B, Comparative biochemistry
SN - 1096-4959
IS - 1
ER -