Expression of two ecdysteroid-regulated genes, Broad-Complex and E75, in the brain and ovary of the honeybee (Apis mellifera L.)

Rajib Kumar Paul, Hideaki Takeuchi, Takeo Kubo

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We previously demonstrated that two ecdysteroid-regulated genes, Mblk-1/E93 and E74, are expressed selectively in Kenyon cell subtypes in the mushroom bodies of the honeybee (Apis mellifera L.) brain. To further examine the possible involvement of ecdysteroid-regulated genes in brain function as well as in oogenesis in the honeybee, we isolated cDNAs for two other ecdysteroid-regulated genes, Broad-Complex (BR-C) and E75, and analyzed their expression in the worker brain as well as in the queen abdomen. In situ hybridization revealed that BR-C, like Mblk-1/E93, is expressed selectively in the large-type Kenyon cells of the mushroom bodies in the worker brain, whereas E75 is expressed in all mushroom body neuron subtypes, suggesting a difference in the mode of response to ecdysteroid among Kenyon cell subtypes. In the queen ovary, both BR-C and £75 are expressed preferentially in the follicle cells that surround egg cells at the late stage, suggesting their role in oogenesis. These results suggest that BR-C and E75 are involved in the regulation of brain function as well as in reproductive physiology in the adult honeybee.

Original languageEnglish
Pages (from-to)1085-1092
Number of pages8
JournalZoological Science
Volume23
Issue number12
DOIs
Publication statusPublished - Dec 2006
Externally publishedYes

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Keywords

  • Broad-Complex
  • E75
  • Ecdysteroid
  • Honeybee
  • Mushroom body
  • Ovary

ASJC Scopus subject areas

  • Animal Science and Zoology

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