TY - JOUR
T1 - Expression of trkc in a mouse osteoblastic cell line and its response to neurotrophin-3
AU - Nakanishi, Tohru
AU - Ohyama, Kazumi
AU - Sogawa, Chiharu
AU - Kudo, Akira
AU - Hattori, Takako
AU - Takahashi, Kojiro
AU - Taniguchi, Shigehiko
AU - Takigawa, Masaharu
PY - 1994/9/15
Y1 - 1994/9/15
N2 - In a cultured osteoblastic cell line, MC3T3-E1 derived from newborn mouse calvaria, the mRNA encoding TRKC, which is the receptor molecule of neurotrophin-3 (NT-3), was detected by the polymerase chain reaction (PCR) method. The mRNAs of the normal type and one alternative form (C14) were highly expressed in the exponential growth phase of MC3T3-E1 cells and decreased as the cells reached the differentiation stage. NT-3, but not nerve growth factor (NGF), stimulated the poliferation of MC3T3-E1 cells in a dose-dependent manner. NT-3 also stimulated calcium incorporation through the surface of MC3T3-E1 cells, indicating the association of NT-3 and its receptor on the cell surface.
AB - In a cultured osteoblastic cell line, MC3T3-E1 derived from newborn mouse calvaria, the mRNA encoding TRKC, which is the receptor molecule of neurotrophin-3 (NT-3), was detected by the polymerase chain reaction (PCR) method. The mRNAs of the normal type and one alternative form (C14) were highly expressed in the exponential growth phase of MC3T3-E1 cells and decreased as the cells reached the differentiation stage. NT-3, but not nerve growth factor (NGF), stimulated the poliferation of MC3T3-E1 cells in a dose-dependent manner. NT-3 also stimulated calcium incorporation through the surface of MC3T3-E1 cells, indicating the association of NT-3 and its receptor on the cell surface.
UR - http://www.scopus.com/inward/record.url?scp=0028110847&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028110847&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1994.2319
DO - 10.1006/bbrc.1994.2319
M3 - Article
C2 - 8093043
AN - SCOPUS:0028110847
SN - 0006-291X
VL - 203
SP - 1268
EP - 1274
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -