Expression of trkC in a Mouse Osteoblastic Cell Line and Its Response to Neurotrophin-3

T. Nakanishi, K. Ohyama, Chiharu Sogawa, A. Kudo, Takako Hattori, K. Takahashi, S. Taniguchi, Masaharu Takigawa

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In a cultured osteoblastic cell line, MC3T3-E1 derived from newborn mouse calvaria, the mRNA encoding TRKC, which is the receptor molecule of neurotrophin-3 (NT-3), was detected by the polymerase chain reaction (PCR) method. The mRNAs of the normal type and one alternative form (C14) were highly expressed in the exponential growth phase of MC3T3-E1 cells and decreased as the cells reached the differentiation stage. NT-3, but not nerve growth factor (NGF), stimulated the poliferation of MC3T3-E1 cells in a dose-dependent manner. NT-3 also stimulated calcium incorporation through the surface of MC3T3-E1 cells, indicating the association of NT-3 and its receptor on the cell surface.

Original languageEnglish
Pages (from-to)1268-1274
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume203
Issue number2
DOIs
Publication statusPublished - Sep 15 1994

Fingerprint

Neurotrophin 3
trkC Receptor
Cells
Cell Line
Messenger RNA
Polymerase chain reaction
Nerve Growth Factor
Skull
Calcium
Cell Differentiation
Cultured Cells
Molecules
Polymerase Chain Reaction
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Expression of trkC in a Mouse Osteoblastic Cell Line and Its Response to Neurotrophin-3. / Nakanishi, T.; Ohyama, K.; Sogawa, Chiharu; Kudo, A.; Hattori, Takako; Takahashi, K.; Taniguchi, S.; Takigawa, Masaharu.

In: Biochemical and Biophysical Research Communications, Vol. 203, No. 2, 15.09.1994, p. 1268-1274.

Research output: Contribution to journalArticle

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