Expression of trkc in a mouse osteoblastic cell line and its response to neurotrophin-3

Tohru Nakanishi, Kazumi Ohyama, Chiharu Sogawa, Akira Kudo, Takako Hattori, Kojiro Takahashi, Shigehiko Taniguchi, Masaharu Takigawa

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


In a cultured osteoblastic cell line, MC3T3-E1 derived from newborn mouse calvaria, the mRNA encoding TRKC, which is the receptor molecule of neurotrophin-3 (NT-3), was detected by the polymerase chain reaction (PCR) method. The mRNAs of the normal type and one alternative form (C14) were highly expressed in the exponential growth phase of MC3T3-E1 cells and decreased as the cells reached the differentiation stage. NT-3, but not nerve growth factor (NGF), stimulated the poliferation of MC3T3-E1 cells in a dose-dependent manner. NT-3 also stimulated calcium incorporation through the surface of MC3T3-E1 cells, indicating the association of NT-3 and its receptor on the cell surface.

Original languageEnglish
Pages (from-to)1268-1274
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - Sept 15 1994

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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