Expression of transcription factor Sox9 in cartilage formation from grafted periosteal cells

Takaaki Ueno, Toshimasa Kagawa, Miwa Kanou, Nobuhisa Ishida, Yoshiro Sakata, Takashi Fujii, Hideaki Imura, Seiji Kondou, Nobuyoshi Mizukawa, Toshio Sugahara

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the expression of Sox9 protein in the process of cartilage formation after periosteal grafting using the rat periosteum graft model. Materials and Methods: Periostea taken from twenty 7-week-old rats were immediately grafted into the suprahyoid muscles. Grafted tissues were harvested 0, 7, 14, and 21 days after grafting and examined by microscopy and immunohistochemistry using CD44, type 2 collagen, and Sox9 antibodies as markers. Results: Periosteal cells expressed CD44 after 7 days and Sox9 after 14 days. The Sox9-positive cells showed chondrogenic differentiation with type 2 collagen expression by 21 days after grafting. Endochondral ossification was also seen 21 days after grafting. Sox9 was not detected in hypertrophic chondrocytes. Conclusion: These findings suggested that Sox9 may be related to chondrogenic differentiation of periosteal cells in grafted periosteum.

Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalAsian Journal of Oral and Maxillofacial Surgery
Volume18
Issue number1
Publication statusPublished - 2006

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Periosteum
Cartilage
Transcription Factors
Collagen Type II
Chondrocytes
Osteogenesis
Cell Differentiation
Microscopy
Immunohistochemistry
Transplants
Muscles
Antibodies
Proteins

Keywords

  • Periosteum
  • Sox-9 transcription factor

ASJC Scopus subject areas

  • Surgery

Cite this

Expression of transcription factor Sox9 in cartilage formation from grafted periosteal cells. / Ueno, Takaaki; Kagawa, Toshimasa; Kanou, Miwa; Ishida, Nobuhisa; Sakata, Yoshiro; Fujii, Takashi; Imura, Hideaki; Kondou, Seiji; Mizukawa, Nobuyoshi; Sugahara, Toshio.

In: Asian Journal of Oral and Maxillofacial Surgery, Vol. 18, No. 1, 2006, p. 35-40.

Research output: Contribution to journalArticle

Ueno, T, Kagawa, T, Kanou, M, Ishida, N, Sakata, Y, Fujii, T, Imura, H, Kondou, S, Mizukawa, N & Sugahara, T 2006, 'Expression of transcription factor Sox9 in cartilage formation from grafted periosteal cells', Asian Journal of Oral and Maxillofacial Surgery, vol. 18, no. 1, pp. 35-40.
Ueno, Takaaki ; Kagawa, Toshimasa ; Kanou, Miwa ; Ishida, Nobuhisa ; Sakata, Yoshiro ; Fujii, Takashi ; Imura, Hideaki ; Kondou, Seiji ; Mizukawa, Nobuyoshi ; Sugahara, Toshio. / Expression of transcription factor Sox9 in cartilage formation from grafted periosteal cells. In: Asian Journal of Oral and Maxillofacial Surgery. 2006 ; Vol. 18, No. 1. pp. 35-40.
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AU - Fujii, Takashi

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AB - Objective: To investigate the expression of Sox9 protein in the process of cartilage formation after periosteal grafting using the rat periosteum graft model. Materials and Methods: Periostea taken from twenty 7-week-old rats were immediately grafted into the suprahyoid muscles. Grafted tissues were harvested 0, 7, 14, and 21 days after grafting and examined by microscopy and immunohistochemistry using CD44, type 2 collagen, and Sox9 antibodies as markers. Results: Periosteal cells expressed CD44 after 7 days and Sox9 after 14 days. The Sox9-positive cells showed chondrogenic differentiation with type 2 collagen expression by 21 days after grafting. Endochondral ossification was also seen 21 days after grafting. Sox9 was not detected in hypertrophic chondrocytes. Conclusion: These findings suggested that Sox9 may be related to chondrogenic differentiation of periosteal cells in grafted periosteum.

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