Abstract
Objective: To investigate the expression of Sox9 protein in the process of cartilage formation after periosteal grafting using the rat periosteum graft model. Materials and Methods: Periostea taken from twenty 7-week-old rats were immediately grafted into the suprahyoid muscles. Grafted tissues were harvested 0, 7, 14, and 21 days after grafting and examined by microscopy and immunohistochemistry using CD44, type 2 collagen, and Sox9 antibodies as markers. Results: Periosteal cells expressed CD44 after 7 days and Sox9 after 14 days. The Sox9-positive cells showed chondrogenic differentiation with type 2 collagen expression by 21 days after grafting. Endochondral ossification was also seen 21 days after grafting. Sox9 was not detected in hypertrophic chondrocytes. Conclusion: These findings suggested that Sox9 may be related to chondrogenic differentiation of periosteal cells in grafted periosteum.
| Original language | English |
|---|---|
| Pages (from-to) | 35-40 |
| Number of pages | 6 |
| Journal | Asian Journal of Oral and Maxillofacial Surgery |
| Volume | 18 |
| Issue number | 1 |
| Publication status | Published - 2006 |
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Keywords
- Periosteum
- Sox-9 transcription factor
ASJC Scopus subject areas
- Surgery
Cite this
Expression of transcription factor Sox9 in cartilage formation from grafted periosteal cells. / Ueno, Takaaki; Kagawa, Toshimasa; Kanou, Miwa; Ishida, Nobuhisa; Sakata, Yoshiro; Fujii, Takashi; Imura, Hideaki; Kondou, Seiji; Mizukawa, Nobuyoshi; Sugahara, Toshio.
In: Asian Journal of Oral and Maxillofacial Surgery, Vol. 18, No. 1, 2006, p. 35-40.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of transcription factor Sox9 in cartilage formation from grafted periosteal cells
AU - Ueno, Takaaki
AU - Kagawa, Toshimasa
AU - Kanou, Miwa
AU - Ishida, Nobuhisa
AU - Sakata, Yoshiro
AU - Fujii, Takashi
AU - Imura, Hideaki
AU - Kondou, Seiji
AU - Mizukawa, Nobuyoshi
AU - Sugahara, Toshio
PY - 2006
Y1 - 2006
N2 - Objective: To investigate the expression of Sox9 protein in the process of cartilage formation after periosteal grafting using the rat periosteum graft model. Materials and Methods: Periostea taken from twenty 7-week-old rats were immediately grafted into the suprahyoid muscles. Grafted tissues were harvested 0, 7, 14, and 21 days after grafting and examined by microscopy and immunohistochemistry using CD44, type 2 collagen, and Sox9 antibodies as markers. Results: Periosteal cells expressed CD44 after 7 days and Sox9 after 14 days. The Sox9-positive cells showed chondrogenic differentiation with type 2 collagen expression by 21 days after grafting. Endochondral ossification was also seen 21 days after grafting. Sox9 was not detected in hypertrophic chondrocytes. Conclusion: These findings suggested that Sox9 may be related to chondrogenic differentiation of periosteal cells in grafted periosteum.
AB - Objective: To investigate the expression of Sox9 protein in the process of cartilage formation after periosteal grafting using the rat periosteum graft model. Materials and Methods: Periostea taken from twenty 7-week-old rats were immediately grafted into the suprahyoid muscles. Grafted tissues were harvested 0, 7, 14, and 21 days after grafting and examined by microscopy and immunohistochemistry using CD44, type 2 collagen, and Sox9 antibodies as markers. Results: Periosteal cells expressed CD44 after 7 days and Sox9 after 14 days. The Sox9-positive cells showed chondrogenic differentiation with type 2 collagen expression by 21 days after grafting. Endochondral ossification was also seen 21 days after grafting. Sox9 was not detected in hypertrophic chondrocytes. Conclusion: These findings suggested that Sox9 may be related to chondrogenic differentiation of periosteal cells in grafted periosteum.
KW - Periosteum
KW - Sox-9 transcription factor
UR - http://www.scopus.com/inward/record.url?scp=33645913558&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645913558&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:33645913558
VL - 18
SP - 35
EP - 40
JO - Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology
JF - Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology
SN - 2212-5558
IS - 1
ER -