Expression of TRAF6 protein and TRAF6 mRNA during different stages of deciduous tooth root physiological absorption

Guirong Zhang, Linxi Zhou, Koji Ito, Yoshihiko Sugita, Keisuke Nakano, Hatsuhiko Maeda, Xue Wang, Minghua Wang

Research output: Contribution to journalArticle

Abstract

The expression of TRAF6 protein and TRAF6 mRNA during different stages of deciduous tooth root physiological absorption was observed. Deciduous teeth with physiological absorption period were divided into 3 groups according to the length of deciduous tooth root absorption: early stage of deciduous tooth root physiological absorption (I group), intermediate stage of deciduous teeth roots' physiological absorption (II group), final stage of deciduous tooth root physiological absorption (III group). TRAF6 antibody and TRAF6 hybridization in situ agent were used to detect the expression of TRAF6 protein and TRAF6 mRNA of odontoclasts of deciduous teeth with different physiological absorption stages. Im-pro-plas 6.0 analytical system software was used to measure the optical density of odontoclasts stained in three groups. SPSS13.0 software was used to analyze the regularity of expression. Optical density value of TRAF6 immunohistochemistry stain and TRAF6 mRNA in situ hybridization stain of odontoclasts was larger in I and II group compared with the value in III group. It indicated that odontoclasts in early and intermediate stages showed more activity than in the final stage. Expression of TRAF6 protein and TRAF6 mRNA during different stages of deciduous tooth root physiological absorption was consistent with the activity of odontoclast.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalJournal of Hard Tissue Biology
Volume22
Issue number1
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

TNF Receptor-Associated Factor 6
Tooth Root
Deciduous Tooth
Proteins
Messenger RNA
Osteoclasts
Density (optical)
Antibodies
In Situ Hybridization
Coloring Agents
Software
Physiological Absorption
Immunohistochemistry

Keywords

  • Odontoclast
  • Root physiological absorption
  • TRAF6
  • TRAF6 mRNA
  • TRAP

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biomaterials
  • Medicine (miscellaneous)
  • Orthopedics and Sports Medicine
  • Dentistry(all)

Cite this

Expression of TRAF6 protein and TRAF6 mRNA during different stages of deciduous tooth root physiological absorption. / Zhang, Guirong; Zhou, Linxi; Ito, Koji; Sugita, Yoshihiko; Nakano, Keisuke; Maeda, Hatsuhiko; Wang, Xue; Wang, Minghua.

In: Journal of Hard Tissue Biology, Vol. 22, No. 1, 2013, p. 1-6.

Research output: Contribution to journalArticle

Zhang, Guirong ; Zhou, Linxi ; Ito, Koji ; Sugita, Yoshihiko ; Nakano, Keisuke ; Maeda, Hatsuhiko ; Wang, Xue ; Wang, Minghua. / Expression of TRAF6 protein and TRAF6 mRNA during different stages of deciduous tooth root physiological absorption. In: Journal of Hard Tissue Biology. 2013 ; Vol. 22, No. 1. pp. 1-6.
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