Expression of Toll-like receptors 2 and 4 on human intestinal lymphatic vessels

Shin Ichiro Kuroshima, Yoshihiko Sawa, Tohru Kawamoto, Yuji Yamaoka, Kenji Notani, Shigemitsu Yoshida, Nobuo Inoue

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The Toll-like receptor (TLR) is a part of the innate immune system sensing pathogen-associated molecular patterns (PAMPs). Recently, TLRs 2 and 4 have been demonstrated for the ligand engagements, which result in the induction of cytokines. Here we investigated the expression of TLRs 2 and 4 on lymphatic vessels producing cys-cys chemokine ligand 21 (CCL21) in the human small intestine. The specificity of antibodies to TLRs was tested on a human monocyte leukemia cell line, umbilical vein endothelial cells (HUVEC), and periodontal ligament fibroblasts (PDLF) with the examination for the TLR gene expression by the reverse transcription-polymerase chain reaction (RT-PCR), and lymphatic vessels were identified by antibodies for platelet-endothelial cell adhesion molecule-1 (PECAM-1) and desmoplakin. The expression of CCL21 was not clearly detected on collecting lymphatic vessels in the submucosa while it was generally observed on the central lacteals of villi and lymphatic capillaries in the lamina propria mucosae. The reaction of antibodies to TLRs 2 and 4 was also not clearly detected on collecting lymphatic vessels in the submucosa and central lacteals of villi, but generally observed on lymphatic capillaries expressing CCL21 in the lamina propria mucosae of tissue where the expression of CCL21 and TLRs was not clearly observed in blood vessels. These may suggest that the expression of CCL21, and TLRs 2 and 4 is predominantly induced in the peripheral lymphatic endothelium of the small intestinal microcirculation. The lymphatic endothelium may contribute to allow dendritic cells to home into secondary lymphoid tissue through the expression of TLRs, the ligand engagements of which result in the induction of chemokines.

Original languageEnglish
Pages (from-to)90-95
Number of pages6
JournalMicrovascular Research
Volume67
Issue number1
DOIs
Publication statusPublished - Jan 2004
Externally publishedYes

Fingerprint

Toll-Like Receptor 2
Toll-Like Receptor 4
Lymphatic Vessels
Chemokines
Ligands
Mucous Membrane
Lymphatic Endothelium
Toll-Like Receptors
cysteinylcysteine
Antibodies
Milk
Desmoplakins
Tissue
CD31 Antigens
Microcirculation
Periodontal Ligament
Umbilical Veins
Antibody Specificity
Immune system
Polymerase chain reaction

Keywords

  • CCL21
  • Lymphatic endothelium
  • Small intestine
  • TLR2
  • TLR4

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

Kuroshima, S. I., Sawa, Y., Kawamoto, T., Yamaoka, Y., Notani, K., Yoshida, S., & Inoue, N. (2004). Expression of Toll-like receptors 2 and 4 on human intestinal lymphatic vessels. Microvascular Research, 67(1), 90-95. https://doi.org/10.1016/j.mvr.2003.09.005

Expression of Toll-like receptors 2 and 4 on human intestinal lymphatic vessels. / Kuroshima, Shin Ichiro; Sawa, Yoshihiko; Kawamoto, Tohru; Yamaoka, Yuji; Notani, Kenji; Yoshida, Shigemitsu; Inoue, Nobuo.

In: Microvascular Research, Vol. 67, No. 1, 01.2004, p. 90-95.

Research output: Contribution to journalArticle

Kuroshima, SI, Sawa, Y, Kawamoto, T, Yamaoka, Y, Notani, K, Yoshida, S & Inoue, N 2004, 'Expression of Toll-like receptors 2 and 4 on human intestinal lymphatic vessels', Microvascular Research, vol. 67, no. 1, pp. 90-95. https://doi.org/10.1016/j.mvr.2003.09.005
Kuroshima, Shin Ichiro ; Sawa, Yoshihiko ; Kawamoto, Tohru ; Yamaoka, Yuji ; Notani, Kenji ; Yoshida, Shigemitsu ; Inoue, Nobuo. / Expression of Toll-like receptors 2 and 4 on human intestinal lymphatic vessels. In: Microvascular Research. 2004 ; Vol. 67, No. 1. pp. 90-95.
@article{034b0e264361403ca5d0622ab51b161f,
title = "Expression of Toll-like receptors 2 and 4 on human intestinal lymphatic vessels",
abstract = "The Toll-like receptor (TLR) is a part of the innate immune system sensing pathogen-associated molecular patterns (PAMPs). Recently, TLRs 2 and 4 have been demonstrated for the ligand engagements, which result in the induction of cytokines. Here we investigated the expression of TLRs 2 and 4 on lymphatic vessels producing cys-cys chemokine ligand 21 (CCL21) in the human small intestine. The specificity of antibodies to TLRs was tested on a human monocyte leukemia cell line, umbilical vein endothelial cells (HUVEC), and periodontal ligament fibroblasts (PDLF) with the examination for the TLR gene expression by the reverse transcription-polymerase chain reaction (RT-PCR), and lymphatic vessels were identified by antibodies for platelet-endothelial cell adhesion molecule-1 (PECAM-1) and desmoplakin. The expression of CCL21 was not clearly detected on collecting lymphatic vessels in the submucosa while it was generally observed on the central lacteals of villi and lymphatic capillaries in the lamina propria mucosae. The reaction of antibodies to TLRs 2 and 4 was also not clearly detected on collecting lymphatic vessels in the submucosa and central lacteals of villi, but generally observed on lymphatic capillaries expressing CCL21 in the lamina propria mucosae of tissue where the expression of CCL21 and TLRs was not clearly observed in blood vessels. These may suggest that the expression of CCL21, and TLRs 2 and 4 is predominantly induced in the peripheral lymphatic endothelium of the small intestinal microcirculation. The lymphatic endothelium may contribute to allow dendritic cells to home into secondary lymphoid tissue through the expression of TLRs, the ligand engagements of which result in the induction of chemokines.",
keywords = "CCL21, Lymphatic endothelium, Small intestine, TLR2, TLR4",
author = "Kuroshima, {Shin Ichiro} and Yoshihiko Sawa and Tohru Kawamoto and Yuji Yamaoka and Kenji Notani and Shigemitsu Yoshida and Nobuo Inoue",
year = "2004",
month = "1",
doi = "10.1016/j.mvr.2003.09.005",
language = "English",
volume = "67",
pages = "90--95",
journal = "Microvascular Research",
issn = "0026-2862",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Expression of Toll-like receptors 2 and 4 on human intestinal lymphatic vessels

AU - Kuroshima, Shin Ichiro

AU - Sawa, Yoshihiko

AU - Kawamoto, Tohru

AU - Yamaoka, Yuji

AU - Notani, Kenji

AU - Yoshida, Shigemitsu

AU - Inoue, Nobuo

PY - 2004/1

Y1 - 2004/1

N2 - The Toll-like receptor (TLR) is a part of the innate immune system sensing pathogen-associated molecular patterns (PAMPs). Recently, TLRs 2 and 4 have been demonstrated for the ligand engagements, which result in the induction of cytokines. Here we investigated the expression of TLRs 2 and 4 on lymphatic vessels producing cys-cys chemokine ligand 21 (CCL21) in the human small intestine. The specificity of antibodies to TLRs was tested on a human monocyte leukemia cell line, umbilical vein endothelial cells (HUVEC), and periodontal ligament fibroblasts (PDLF) with the examination for the TLR gene expression by the reverse transcription-polymerase chain reaction (RT-PCR), and lymphatic vessels were identified by antibodies for platelet-endothelial cell adhesion molecule-1 (PECAM-1) and desmoplakin. The expression of CCL21 was not clearly detected on collecting lymphatic vessels in the submucosa while it was generally observed on the central lacteals of villi and lymphatic capillaries in the lamina propria mucosae. The reaction of antibodies to TLRs 2 and 4 was also not clearly detected on collecting lymphatic vessels in the submucosa and central lacteals of villi, but generally observed on lymphatic capillaries expressing CCL21 in the lamina propria mucosae of tissue where the expression of CCL21 and TLRs was not clearly observed in blood vessels. These may suggest that the expression of CCL21, and TLRs 2 and 4 is predominantly induced in the peripheral lymphatic endothelium of the small intestinal microcirculation. The lymphatic endothelium may contribute to allow dendritic cells to home into secondary lymphoid tissue through the expression of TLRs, the ligand engagements of which result in the induction of chemokines.

AB - The Toll-like receptor (TLR) is a part of the innate immune system sensing pathogen-associated molecular patterns (PAMPs). Recently, TLRs 2 and 4 have been demonstrated for the ligand engagements, which result in the induction of cytokines. Here we investigated the expression of TLRs 2 and 4 on lymphatic vessels producing cys-cys chemokine ligand 21 (CCL21) in the human small intestine. The specificity of antibodies to TLRs was tested on a human monocyte leukemia cell line, umbilical vein endothelial cells (HUVEC), and periodontal ligament fibroblasts (PDLF) with the examination for the TLR gene expression by the reverse transcription-polymerase chain reaction (RT-PCR), and lymphatic vessels were identified by antibodies for platelet-endothelial cell adhesion molecule-1 (PECAM-1) and desmoplakin. The expression of CCL21 was not clearly detected on collecting lymphatic vessels in the submucosa while it was generally observed on the central lacteals of villi and lymphatic capillaries in the lamina propria mucosae. The reaction of antibodies to TLRs 2 and 4 was also not clearly detected on collecting lymphatic vessels in the submucosa and central lacteals of villi, but generally observed on lymphatic capillaries expressing CCL21 in the lamina propria mucosae of tissue where the expression of CCL21 and TLRs was not clearly observed in blood vessels. These may suggest that the expression of CCL21, and TLRs 2 and 4 is predominantly induced in the peripheral lymphatic endothelium of the small intestinal microcirculation. The lymphatic endothelium may contribute to allow dendritic cells to home into secondary lymphoid tissue through the expression of TLRs, the ligand engagements of which result in the induction of chemokines.

KW - CCL21

KW - Lymphatic endothelium

KW - Small intestine

KW - TLR2

KW - TLR4

UR - http://www.scopus.com/inward/record.url?scp=0347989364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0347989364&partnerID=8YFLogxK

U2 - 10.1016/j.mvr.2003.09.005

DO - 10.1016/j.mvr.2003.09.005

M3 - Article

C2 - 14709406

AN - SCOPUS:0347989364

VL - 67

SP - 90

EP - 95

JO - Microvascular Research

JF - Microvascular Research

SN - 0026-2862

IS - 1

ER -