Expression of the hepatocyte growth factor gene during chick limb development

Fumio Myokai, Norifumi Washio, Yoji Asahara, Tomoichiro Yamaai, Nobuya Tanda, Tetsuya Ishikawa, Shunsuke Aoki, Hidemi Kurihara, Yoji Murayama, Taiichi Saito, Kunio Matsumoto, Toshikazu Nakamura, Sumihare Noji, Tsutomu Nohno

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37 Citations (Scopus)

Abstract

It has been shown that mirrorimage duplications of the zeugopodia and digits are formed when MRC‐5 fibroblasts producing hepatocyte growth factor (HGF) are applied to the anterior region of the chick limb bud (Yonei et al. [1993] Dev. Biol. 160:246–253). To evaluate the role of HGF in limb development, we observed the expression pattern of the HGF gene using in situ hybridization. The HGF gene was expressed in the mesoderm of the limb bud and in the central core region of mandibular arch and maxillary processes at stages 17 to 24. When both wing and leg buds begin to extend distally, the HGF gene is expressed in the mesenchymal cells, but not in the ectodermal cells and somites. Concomitant with establishment of the apical ectodermal ridge, distal mesenchymal cells of the limb bud express the HGF gene intensely with a gradient higher in the distal region. The HGF expression is later confined to the ventral and subapical mesenchyme of the limb bud, although no signal is detectable in the apical and non‐ridge ectoderm. However, signal for the c‐met proto‐oncogene encoding the HGF receptor is not detectable in the limb bud at stages 17 to 24. These results suggest that HGF produced in the limb mesoderm may be involved in initial induction and maintenance of the apical ectoderm during limb development. © 1995 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)80-90
Number of pages11
JournalDevelopmental Dynamics
Volume202
Issue number1
DOIs
Publication statusPublished - Jan 1995
Externally publishedYes

Keywords

  • Chick embryo
  • Gene expression
  • Hepatocyte growth factor
  • In situ hybridization
  • Limb development
  • c‐met

ASJC Scopus subject areas

  • Developmental Biology

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  • Cite this

    Myokai, F., Washio, N., Asahara, Y., Yamaai, T., Tanda, N., Ishikawa, T., Aoki, S., Kurihara, H., Murayama, Y., Saito, T., Matsumoto, K., Nakamura, T., Noji, S., & Nohno, T. (1995). Expression of the hepatocyte growth factor gene during chick limb development. Developmental Dynamics, 202(1), 80-90. https://doi.org/10.1002/aja.1002020108