Abstract
Change of the glial cell line-derived neurotrophic factor (GDNF) gene expression in rat brain was examined after transient middle cerebral artery (MCA) occlusion of adult rats. Northern blot analysis showed that the mRNA began to be induced in the occluded MCA from 1 h of reperfusion with a peak at 3 h, and almost diminished by 1 day of reperfusion. Immunohistochemical analysis with brain sections showed an expression of GDNF-like immunoreactivity in neurons of the cerebral cortex and caudate after 90 min of ischemia in a similar way to the mRNA, but the staining was moro disseminated and stronger in the cerebral cortex than the caudate. No glial cell was stained in the brain sections. The present results indicate that the GDNF gene was expressed in an early stage of reperfusion in neuronal cells of the MCA territory, but that the staining property was different between in the cerebral cortex and caudate.
| Original language | English |
|---|---|
| Pages (from-to) | 230-234 |
| Number of pages | 5 |
| Journal | Brain Research |
| Volume | 776 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Nov 21 1997 |
| Externally published | Yes |
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Keywords
- GDNF
- Ischemia
- Middle cerebral artery
- Rat
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Expression of the glial cell line-derived neurotrophic factor gene in rat brain after transient MCA occlusion. / Abe, Koji; Hayashi, T.
In: Brain Research, Vol. 776, No. 1-2, 21.11.1997, p. 230-234.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of the glial cell line-derived neurotrophic factor gene in rat brain after transient MCA occlusion
AU - Abe, Koji
AU - Hayashi, T.
PY - 1997/11/21
Y1 - 1997/11/21
N2 - Change of the glial cell line-derived neurotrophic factor (GDNF) gene expression in rat brain was examined after transient middle cerebral artery (MCA) occlusion of adult rats. Northern blot analysis showed that the mRNA began to be induced in the occluded MCA from 1 h of reperfusion with a peak at 3 h, and almost diminished by 1 day of reperfusion. Immunohistochemical analysis with brain sections showed an expression of GDNF-like immunoreactivity in neurons of the cerebral cortex and caudate after 90 min of ischemia in a similar way to the mRNA, but the staining was moro disseminated and stronger in the cerebral cortex than the caudate. No glial cell was stained in the brain sections. The present results indicate that the GDNF gene was expressed in an early stage of reperfusion in neuronal cells of the MCA territory, but that the staining property was different between in the cerebral cortex and caudate.
AB - Change of the glial cell line-derived neurotrophic factor (GDNF) gene expression in rat brain was examined after transient middle cerebral artery (MCA) occlusion of adult rats. Northern blot analysis showed that the mRNA began to be induced in the occluded MCA from 1 h of reperfusion with a peak at 3 h, and almost diminished by 1 day of reperfusion. Immunohistochemical analysis with brain sections showed an expression of GDNF-like immunoreactivity in neurons of the cerebral cortex and caudate after 90 min of ischemia in a similar way to the mRNA, but the staining was moro disseminated and stronger in the cerebral cortex than the caudate. No glial cell was stained in the brain sections. The present results indicate that the GDNF gene was expressed in an early stage of reperfusion in neuronal cells of the MCA territory, but that the staining property was different between in the cerebral cortex and caudate.
KW - GDNF
KW - Ischemia
KW - Middle cerebral artery
KW - Rat
UR - http://www.scopus.com/inward/record.url?scp=0031458886&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031458886&partnerID=8YFLogxK
U2 - 10.1016/S0006-8993(97)01041-X
DO - 10.1016/S0006-8993(97)01041-X
M3 - Article
C2 - 9439817
AN - SCOPUS:0031458886
VL - 776
SP - 230
EP - 234
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -