TY - JOUR
T1 - Expression of the glial cell line-derived neurotrophic factor gene in rat brain after transient MCA occlusion
AU - Abe, K.
AU - Hayashi, T.
N1 - Funding Information:
This work was partly supported by Grant-in-Aid for Scientific Research on Priority Areas (Kanazawa I.) 07264204 and 08256204, and Grant-in-Aid for Scientific Research (B) 09470151 and (C) 06807055 from the Ministry of Education, Science and Culture of Japan.
PY - 1997/11/21
Y1 - 1997/11/21
N2 - Change of the glial cell line-derived neurotrophic factor (GDNF) gene expression in rat brain was examined after transient middle cerebral artery (MCA) occlusion of adult rats. Northern blot analysis showed that the mRNA began to be induced in the occluded MCA from 1 h of reperfusion with a peak at 3 h, and almost diminished by 1 day of reperfusion. Immunohistochemical analysis with brain sections showed an expression of GDNF-like immunoreactivity in neurons of the cerebral cortex and caudate after 90 min of ischemia in a similar way to the mRNA, but the staining was moro disseminated and stronger in the cerebral cortex than the caudate. No glial cell was stained in the brain sections. The present results indicate that the GDNF gene was expressed in an early stage of reperfusion in neuronal cells of the MCA territory, but that the staining property was different between in the cerebral cortex and caudate.
AB - Change of the glial cell line-derived neurotrophic factor (GDNF) gene expression in rat brain was examined after transient middle cerebral artery (MCA) occlusion of adult rats. Northern blot analysis showed that the mRNA began to be induced in the occluded MCA from 1 h of reperfusion with a peak at 3 h, and almost diminished by 1 day of reperfusion. Immunohistochemical analysis with brain sections showed an expression of GDNF-like immunoreactivity in neurons of the cerebral cortex and caudate after 90 min of ischemia in a similar way to the mRNA, but the staining was moro disseminated and stronger in the cerebral cortex than the caudate. No glial cell was stained in the brain sections. The present results indicate that the GDNF gene was expressed in an early stage of reperfusion in neuronal cells of the MCA territory, but that the staining property was different between in the cerebral cortex and caudate.
KW - GDNF
KW - Ischemia
KW - Middle cerebral artery
KW - Rat
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U2 - 10.1016/S0006-8993(97)01041-X
DO - 10.1016/S0006-8993(97)01041-X
M3 - Article
C2 - 9439817
AN - SCOPUS:0031458886
VL - 776
SP - 230
EP - 234
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1-2
ER -