Expression of the cDNA encoding lipocortin-like 39 kDa protein of guinea pig neutrophils in yeast. Purification and biological characterization

Eisuke F. Sato; Yoshikazu Tanaka; Keisuke Edashige; Hirotsugu Kobuchi; Seiji Morishita; Yasuo M. Sugino; Masayasu Inoue; Kozo Utsumi

doi:10.1016/0014-5793(89)81097-X

Expression of the cDNA encoding lipocortin-like 39 kDa protein of guinea pig neutrophils in yeast. Purification and biological characterization

Eisuke F. Sato, Yoshikazu Tanaka, Keisuke Edashige, Hirotsugu Kobuchi, Seiji Morishita, Yasuo M. Sugino, Masayasu Inoue, Kozo Utsumi

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The cDNA encoding lipocortin-like 39 kDa protein in guinea pig neutrophils was cloned into a yeast expression vector and the constructed plasmid was introduced into a yeast. The gene was expressed in an eukaryotic cell, yeast Saccharomyces cerevisiae and the recombinant protein was purified and characterized. The purified protein was identical with the native one with respect to the antigenicity and several biochemical properties, such as inhibitory action against phospholipase A₂, Ca²⁺-dependent binding to acidic-phospholipids and F-actin and availability as a substrate for tyrosine kinase (EGF receptor/kinase) and protein kinase C.

Original language	English
Pages (from-to)	231-236
Number of pages	6
Journal	FEBS Letters
Volume	255
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(89)81097-X
Publication status	Published - Sep 25 1989
Externally published	Yes

Keywords

Lipocortin
Neutrophil
Protein kinase
Recombinant protein

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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Expression of the cDNA encoding lipocortin-like 39 kDa protein of guinea pig neutrophils in yeast. Purification and biological characterization. / Sato, Eisuke F.; Tanaka, Yoshikazu; Edashige, Keisuke; Kobuchi, Hirotsugu; Morishita, Seiji; Sugino, Yasuo M.; Inoue, Masayasu; Utsumi, Kozo.

In: FEBS Letters, Vol. 255, No. 2, 25.09.1989, p. 231-236.

Research output: Contribution to journal › Article › peer-review

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title = "Expression of the cDNA encoding lipocortin-like 39 kDa protein of guinea pig neutrophils in yeast. Purification and biological characterization",

abstract = "The cDNA encoding lipocortin-like 39 kDa protein in guinea pig neutrophils was cloned into a yeast expression vector and the constructed plasmid was introduced into a yeast. The gene was expressed in an eukaryotic cell, yeast Saccharomyces cerevisiae and the recombinant protein was purified and characterized. The purified protein was identical with the native one with respect to the antigenicity and several biochemical properties, such as inhibitory action against phospholipase A2, Ca2+-dependent binding to acidic-phospholipids and F-actin and availability as a substrate for tyrosine kinase (EGF receptor/kinase) and protein kinase C.",

keywords = "Lipocortin, Neutrophil, Protein kinase, Recombinant protein",

author = "Sato, {Eisuke F.} and Yoshikazu Tanaka and Keisuke Edashige and Hirotsugu Kobuchi and Seiji Morishita and Sugino, {Yasuo M.} and Masayasu Inoue and Kozo Utsumi",

note = "Funding Information: AcknowledgementTsh: is work was supportedb y a Grant-in-Aid from the Ministry of Education, Sciencea nd Culture of Japan. The authorsw ish to thank Dr K. Owadaf or the gift of A-431 cells and Miss Yumi Kitaoka for preparing the manuscript.",

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AU - Sato, Eisuke F.

AU - Tanaka, Yoshikazu

AU - Edashige, Keisuke

AU - Kobuchi, Hirotsugu

AU - Morishita, Seiji

AU - Sugino, Yasuo M.

AU - Inoue, Masayasu

AU - Utsumi, Kozo

N1 - Funding Information: AcknowledgementTsh: is work was supportedb y a Grant-in-Aid from the Ministry of Education, Sciencea nd Culture of Japan. The authorsw ish to thank Dr K. Owadaf or the gift of A-431 cells and Miss Yumi Kitaoka for preparing the manuscript.

PY - 1989/9/25

Y1 - 1989/9/25

N2 - The cDNA encoding lipocortin-like 39 kDa protein in guinea pig neutrophils was cloned into a yeast expression vector and the constructed plasmid was introduced into a yeast. The gene was expressed in an eukaryotic cell, yeast Saccharomyces cerevisiae and the recombinant protein was purified and characterized. The purified protein was identical with the native one with respect to the antigenicity and several biochemical properties, such as inhibitory action against phospholipase A2, Ca2+-dependent binding to acidic-phospholipids and F-actin and availability as a substrate for tyrosine kinase (EGF receptor/kinase) and protein kinase C.

AB - The cDNA encoding lipocortin-like 39 kDa protein in guinea pig neutrophils was cloned into a yeast expression vector and the constructed plasmid was introduced into a yeast. The gene was expressed in an eukaryotic cell, yeast Saccharomyces cerevisiae and the recombinant protein was purified and characterized. The purified protein was identical with the native one with respect to the antigenicity and several biochemical properties, such as inhibitory action against phospholipase A2, Ca2+-dependent binding to acidic-phospholipids and F-actin and availability as a substrate for tyrosine kinase (EGF receptor/kinase) and protein kinase C.

KW - Lipocortin

KW - Neutrophil

KW - Protein kinase

KW - Recombinant protein

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EP - 236

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

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Expression of the cDNA encoding lipocortin-like 39 kDa protein of guinea pig neutrophils in yeast. Purification and biological characterization

Abstract

Keywords

ASJC Scopus subject areas

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