Abstract
The cDNA encoding lipocortin-like 39 kDa protein in guinea pig neutrophils was cloned into a yeast expression vector and the constructed plasmid was introduced into a yeast. The gene was expressed in an eukaryotic cell, yeast Saccharomyces cerevisiae and the recombinant protein was purified and characterized. The purified protein was identical with the native one with respect to the antigenicity and several biochemical properties, such as inhibitory action against phospholipase A2, Ca2+-dependent binding to acidic-phospholipids and F-actin and availability as a substrate for tyrosine kinase (EGF receptor/kinase) and protein kinase C.
| Original language | English |
|---|---|
| Pages (from-to) | 231-236 |
| Number of pages | 6 |
| Journal | FEBS Letters |
| Volume | 255 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Sep 25 1989 |
| Externally published | Yes |
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Keywords
- Lipocortin
- Neutrophil
- Protein kinase
- Recombinant protein
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Expression of the cDNA encoding lipocortin-like 39 kDa protein of guinea pig neutrophils in yeast. Purification and biological characterization. / Sato, Eisuke F.; Tanaka, Yoshikazu; Edashige, Keisuke; Kobuchi, Hirotsugu; Morishita, Seiji; Sugino, Yasuo M.; Inoue, Masayasu; Utsumi, Kozo.
In: FEBS Letters, Vol. 255, No. 2, 25.09.1989, p. 231-236.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of the cDNA encoding lipocortin-like 39 kDa protein of guinea pig neutrophils in yeast. Purification and biological characterization
AU - Sato, Eisuke F.
AU - Tanaka, Yoshikazu
AU - Edashige, Keisuke
AU - Kobuchi, Hirotsugu
AU - Morishita, Seiji
AU - Sugino, Yasuo M.
AU - Inoue, Masayasu
AU - Utsumi, Kozo
PY - 1989/9/25
Y1 - 1989/9/25
N2 - The cDNA encoding lipocortin-like 39 kDa protein in guinea pig neutrophils was cloned into a yeast expression vector and the constructed plasmid was introduced into a yeast. The gene was expressed in an eukaryotic cell, yeast Saccharomyces cerevisiae and the recombinant protein was purified and characterized. The purified protein was identical with the native one with respect to the antigenicity and several biochemical properties, such as inhibitory action against phospholipase A2, Ca2+-dependent binding to acidic-phospholipids and F-actin and availability as a substrate for tyrosine kinase (EGF receptor/kinase) and protein kinase C.
AB - The cDNA encoding lipocortin-like 39 kDa protein in guinea pig neutrophils was cloned into a yeast expression vector and the constructed plasmid was introduced into a yeast. The gene was expressed in an eukaryotic cell, yeast Saccharomyces cerevisiae and the recombinant protein was purified and characterized. The purified protein was identical with the native one with respect to the antigenicity and several biochemical properties, such as inhibitory action against phospholipase A2, Ca2+-dependent binding to acidic-phospholipids and F-actin and availability as a substrate for tyrosine kinase (EGF receptor/kinase) and protein kinase C.
KW - Lipocortin
KW - Neutrophil
KW - Protein kinase
KW - Recombinant protein
UR - http://www.scopus.com/inward/record.url?scp=0024310103&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024310103&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(89)81097-X
DO - 10.1016/0014-5793(89)81097-X
M3 - Article
C2 - 2529138
AN - SCOPUS:0024310103
VL - 255
SP - 231
EP - 236
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -