Expression of the cDNA encoding lipocortin-like 39 kDa protein of guinea pig neutrophils in yeast. Purification and biological characterization

Eisuke F. Sato, Yoshikazu Tanaka, Keisuke Edashige, Hirotsugu Kobuchi, Seiji Morishita, Yasuo M. Sugino, Masayasu Inoue, Kozo Utsumi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The cDNA encoding lipocortin-like 39 kDa protein in guinea pig neutrophils was cloned into a yeast expression vector and the constructed plasmid was introduced into a yeast. The gene was expressed in an eukaryotic cell, yeast Saccharomyces cerevisiae and the recombinant protein was purified and characterized. The purified protein was identical with the native one with respect to the antigenicity and several biochemical properties, such as inhibitory action against phospholipase A2, Ca2+-dependent binding to acidic-phospholipids and F-actin and availability as a substrate for tyrosine kinase (EGF receptor/kinase) and protein kinase C.

Original languageEnglish
Pages (from-to)231-236
Number of pages6
JournalFEBS Letters
Volume255
Issue number2
DOIs
Publication statusPublished - Sep 25 1989
Externally publishedYes

Fingerprint

Annexins
Yeast
Purification
Guinea Pigs
Neutrophils
Complementary DNA
Yeasts
Saccharomyces cerevisiae Proteins
Fungal Proteins
Phospholipases A2
Eukaryotic Cells
Recombinant Proteins
Epidermal Growth Factor Receptor
Protein-Tyrosine Kinases
Actins
Phospholipids
Proteins
Plasmids
Genes
Availability

Keywords

  • Lipocortin
  • Neutrophil
  • Protein kinase
  • Recombinant protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Expression of the cDNA encoding lipocortin-like 39 kDa protein of guinea pig neutrophils in yeast. Purification and biological characterization. / Sato, Eisuke F.; Tanaka, Yoshikazu; Edashige, Keisuke; Kobuchi, Hirotsugu; Morishita, Seiji; Sugino, Yasuo M.; Inoue, Masayasu; Utsumi, Kozo.

In: FEBS Letters, Vol. 255, No. 2, 25.09.1989, p. 231-236.

Research output: Contribution to journalArticle

Sato, Eisuke F. ; Tanaka, Yoshikazu ; Edashige, Keisuke ; Kobuchi, Hirotsugu ; Morishita, Seiji ; Sugino, Yasuo M. ; Inoue, Masayasu ; Utsumi, Kozo. / Expression of the cDNA encoding lipocortin-like 39 kDa protein of guinea pig neutrophils in yeast. Purification and biological characterization. In: FEBS Letters. 1989 ; Vol. 255, No. 2. pp. 231-236.
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