Expression of the cadherin-11 gene is a discriminative factor between articular and growth plate chondrocytes

Takashi Matsusaki, T. Aoyama, K. Nishijo, T. Okamoto, T. Nakayama, T. Nakamura, J. Toguchida

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objective: Calcification of hypertrophic chondrocytes is the final step in the differentiation of growth plates, although the precise mechanism is not known. We have established two growth plate-derived chondrocyte cell lines, MMR14 and MMR17, from p53-/- mice (Nakamata T, Aoyama T, Okamoto T, Hosaka T, Nishijo K, Nakayama T, et al. In vitro demonstration of cell-to-cell interaction in growth plate cartilage using chondrocytes established from p53-/- mice. J Bone Miner Res 2003;18:97-107). Prolonged in vitro culture produced calcified nodules in MMR14, but not in MMR17. Factors responsible for the difference in calcification between the two cell lines may also be involved in the physiological calcification in growth plate. Design: Gene expression profiles of MMR14 and MMR17 were compared using a cDNA microarray to identify candidate genes involved in the calcification process. Results: Forty-five genes were identified as upregulated in MMR14, including the cadherin-11 (Cdh-11) gene. The expression of Cdh-11 in MMR14 was detected in cell-cell junctions, while no expression was observed in MMR17. Primary cultured chondrocytes from growth plate (GC) also expressed the Cdh-11, and the staining of Cdh-11 was observed in the late hypertrophic zone of growth plate. Cell aggregation assays showed that chondrocytes required Ca2+ to form nodules, and knockdown of the Cdh-11 gene expression using short interfering RNA inhibited the formation of calcified nodules in MMR14. The introduction of Cdh-11 into MMR17 failed to produce calcified nodules indicating that Cdh-11 is one, but not the sole, factor responsible for the production of calcified nodules. Conclusion: Although the physiological role is still unclear, Cdh-11 is a discriminative factor between articular and growth plate chondrocytes.

Original languageEnglish
Pages (from-to)353-366
Number of pages14
JournalOsteoarthritis and Cartilage
Volume14
Issue number4
DOIs
Publication statusPublished - Apr 2006
Externally publishedYes

Fingerprint

Growth Plate
Chondrocytes
Genes
Joints
Gene expression
Cells
Miners
Cell Line
Physiologic Calcification
Cell Aggregation
Cartilage
Microarrays
Intercellular Junctions
osteoblast cadherin
RNA
Oligonucleotide Array Sequence Analysis
Transcriptome
Assays
Cell Communication
Bone

Keywords

  • Cadherin-11
  • Calcification
  • Chondrocyte
  • Growth plate

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Expression of the cadherin-11 gene is a discriminative factor between articular and growth plate chondrocytes. / Matsusaki, Takashi; Aoyama, T.; Nishijo, K.; Okamoto, T.; Nakayama, T.; Nakamura, T.; Toguchida, J.

In: Osteoarthritis and Cartilage, Vol. 14, No. 4, 04.2006, p. 353-366.

Research output: Contribution to journalArticle

Matsusaki, Takashi ; Aoyama, T. ; Nishijo, K. ; Okamoto, T. ; Nakayama, T. ; Nakamura, T. ; Toguchida, J. / Expression of the cadherin-11 gene is a discriminative factor between articular and growth plate chondrocytes. In: Osteoarthritis and Cartilage. 2006 ; Vol. 14, No. 4. pp. 353-366.
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AU - Matsusaki, Takashi

AU - Aoyama, T.

AU - Nishijo, K.

AU - Okamoto, T.

AU - Nakayama, T.

AU - Nakamura, T.

AU - Toguchida, J.

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N2 - Objective: Calcification of hypertrophic chondrocytes is the final step in the differentiation of growth plates, although the precise mechanism is not known. We have established two growth plate-derived chondrocyte cell lines, MMR14 and MMR17, from p53-/- mice (Nakamata T, Aoyama T, Okamoto T, Hosaka T, Nishijo K, Nakayama T, et al. In vitro demonstration of cell-to-cell interaction in growth plate cartilage using chondrocytes established from p53-/- mice. J Bone Miner Res 2003;18:97-107). Prolonged in vitro culture produced calcified nodules in MMR14, but not in MMR17. Factors responsible for the difference in calcification between the two cell lines may also be involved in the physiological calcification in growth plate. Design: Gene expression profiles of MMR14 and MMR17 were compared using a cDNA microarray to identify candidate genes involved in the calcification process. Results: Forty-five genes were identified as upregulated in MMR14, including the cadherin-11 (Cdh-11) gene. The expression of Cdh-11 in MMR14 was detected in cell-cell junctions, while no expression was observed in MMR17. Primary cultured chondrocytes from growth plate (GC) also expressed the Cdh-11, and the staining of Cdh-11 was observed in the late hypertrophic zone of growth plate. Cell aggregation assays showed that chondrocytes required Ca2+ to form nodules, and knockdown of the Cdh-11 gene expression using short interfering RNA inhibited the formation of calcified nodules in MMR14. The introduction of Cdh-11 into MMR17 failed to produce calcified nodules indicating that Cdh-11 is one, but not the sole, factor responsible for the production of calcified nodules. Conclusion: Although the physiological role is still unclear, Cdh-11 is a discriminative factor between articular and growth plate chondrocytes.

AB - Objective: Calcification of hypertrophic chondrocytes is the final step in the differentiation of growth plates, although the precise mechanism is not known. We have established two growth plate-derived chondrocyte cell lines, MMR14 and MMR17, from p53-/- mice (Nakamata T, Aoyama T, Okamoto T, Hosaka T, Nishijo K, Nakayama T, et al. In vitro demonstration of cell-to-cell interaction in growth plate cartilage using chondrocytes established from p53-/- mice. J Bone Miner Res 2003;18:97-107). Prolonged in vitro culture produced calcified nodules in MMR14, but not in MMR17. Factors responsible for the difference in calcification between the two cell lines may also be involved in the physiological calcification in growth plate. Design: Gene expression profiles of MMR14 and MMR17 were compared using a cDNA microarray to identify candidate genes involved in the calcification process. Results: Forty-five genes were identified as upregulated in MMR14, including the cadherin-11 (Cdh-11) gene. The expression of Cdh-11 in MMR14 was detected in cell-cell junctions, while no expression was observed in MMR17. Primary cultured chondrocytes from growth plate (GC) also expressed the Cdh-11, and the staining of Cdh-11 was observed in the late hypertrophic zone of growth plate. Cell aggregation assays showed that chondrocytes required Ca2+ to form nodules, and knockdown of the Cdh-11 gene expression using short interfering RNA inhibited the formation of calcified nodules in MMR14. The introduction of Cdh-11 into MMR17 failed to produce calcified nodules indicating that Cdh-11 is one, but not the sole, factor responsible for the production of calcified nodules. Conclusion: Although the physiological role is still unclear, Cdh-11 is a discriminative factor between articular and growth plate chondrocytes.

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KW - Calcification

KW - Chondrocyte

KW - Growth plate

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