Expression of the 12-oxophytodienoic acid 10,11-reductase gene in the compatible interaction between pea and fungal pathogen

Yasuhiro Ishiga, Akiko Funato, Tomoyuki Tachiki, Kazuhiro Toyoda, Tomonori Shiraishi, Tetsuji Yamada, Yuki Ichinose

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Suppressors produced by Mycosphaerella pinodes are glycopeptides to block pea defense responses induced by elicitors. A clone, S64, was isolated as cDNA for suppressor-inducible gene from pea epicotyls. The treatment of pea epicotyls with suppressor alone induced an increase of S64 mRNA within 1 h, and it reached a maximum level at 3 h after treatment. The induction was not affected by application of the elicitor, indicating that the suppressor has a dominant action to regulate S64 gene expression. S64 was also induced by inoculation with a virulent pathogen, M. pinodes, but not by inoculation with a non-pathogen, Ascochyta rabid, nor by treatment with fungal elicitor. The deduced structure of S64 showed high homology to 12-oxophytodienoic acid reductase (OPR) in Arabidopsis thaliana. A recombinant protein derived from S64 had OPR activity, suggesting compatibility-specific activation of the octadecanoid pathway in plants. Treatment with jasmonic acid (JA) or methyl jasmonic acid, end products of the octadecanoid pathway, inhibited the elicitor-induced accumulation of PAL mRNA in pea. These results indicate that the suppressor-induced S64 gene expression leads to the production of JA or related compounds, which might contribute to the establishment of compatibility by inhibiting the phenylpropanoid biosynthetic pathway.

Original languageEnglish
Pages (from-to)1210-1220
Number of pages11
JournalPlant and Cell Physiology
Volume43
Issue number10
Publication statusPublished - Oct 1 2002

Fingerprint

Peas
peas
Oxidoreductases
Mycosphaerella pinodes
epicotyls
pathogens
acids
jasmonic acid
Genes
genes
Suppressor Genes
glycopeptides
Gene Expression
Ascochyta
gene expression
Messenger RNA
Glycopeptides
Biosynthetic Pathways
methyl jasmonate
phenylpropanoids

Keywords

  • Jasmonic acid
  • Oxophytodienoic acid reductase
  • Suppressor

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Expression of the 12-oxophytodienoic acid 10,11-reductase gene in the compatible interaction between pea and fungal pathogen. / Ishiga, Yasuhiro; Funato, Akiko; Tachiki, Tomoyuki; Toyoda, Kazuhiro; Shiraishi, Tomonori; Yamada, Tetsuji; Ichinose, Yuki.

In: Plant and Cell Physiology, Vol. 43, No. 10, 01.10.2002, p. 1210-1220.

Research output: Contribution to journalArticle

Ishiga, Yasuhiro ; Funato, Akiko ; Tachiki, Tomoyuki ; Toyoda, Kazuhiro ; Shiraishi, Tomonori ; Yamada, Tetsuji ; Ichinose, Yuki. / Expression of the 12-oxophytodienoic acid 10,11-reductase gene in the compatible interaction between pea and fungal pathogen. In: Plant and Cell Physiology. 2002 ; Vol. 43, No. 10. pp. 1210-1220.
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