Expression of Ten-m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice

Takashi Murakami, Tomohiro Fukunaga, Nobuo Takeshita, Koichi Hiratsuka, Yoshimitsu Abiko, Takashi Yamashiro, Teruko Takano-Yamamoto

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

It has been speculated that the mandibular condyle develops via the differentiation of the fibroblast-like cells covering the condyle into chondrocytes; however, the developmental mechanisms behind this process have not been revealed. We used laser-capture microdissection and cDNA microarray analysis to elucidate the genes that are highly expressed in these fibroblast-like cells. Among these genes, the transcription of Ten-m/Odz3 was significantly increased in the fibroblast-like cells compared with other cartilage tissues. For the first time, we describe the temporal and spatial expression of Ten-m/Odz3 mRNA in relation to the expression of type I, II, and X collagen mRNA, as determined by in-situ hybridization in mouse mandibular condylar cartilage and mouse femoral cartilage during the early stages of development. Ten-m/Odz3 was expressed in the fibrous layer and the proliferating and mature chondrocyte layers, which expressed type I and II collagen, respectively, but was not detected in the hypertrophic chondrocyte layer. Furthermore, we evaluated the in-vitro expression of Ten-m/Odz3 using ATDC5 cells, a mouse chondrogenic cell line. Ten-m/Odz3 was expressed during the early stage of the differentiation of mesenchymal cells into chondrocytes. These findings suggest that Ten-m/Odz3 is involved in the differentiation of chondrocytes and that it acts as a regulatory factor in the early stages of the development of mandibular condylar cartilage.

Original languageEnglish
Pages (from-to)236-244
Number of pages9
JournalJournal of Anatomy
Volume217
Issue number3
DOIs
Publication statusPublished - Sep 2010

Fingerprint

chondrocytes
Chondrocytes
cartilage
Cartilage
collagen
mice
fibroblasts
Growth
Collagen Type II
Fibroblasts
Collagen Type I
gene
Collagen Type X
cells
Laser Capture Microdissection
Mandibular Condyle
laser
Messenger RNA
Microarray Analysis
thighs

Keywords

  • Cartilage
  • Chondrocyte
  • Gene expression
  • Odd Oz
  • Tenascin-like molecule major

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Developmental Biology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Cell Biology

Cite this

Murakami, T., Fukunaga, T., Takeshita, N., Hiratsuka, K., Abiko, Y., Yamashiro, T., & Takano-Yamamoto, T. (2010). Expression of Ten-m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice. Journal of Anatomy, 217(3), 236-244. https://doi.org/10.1111/j.1469-7580.2010.01267.x

Expression of Ten-m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice. / Murakami, Takashi; Fukunaga, Tomohiro; Takeshita, Nobuo; Hiratsuka, Koichi; Abiko, Yoshimitsu; Yamashiro, Takashi; Takano-Yamamoto, Teruko.

In: Journal of Anatomy, Vol. 217, No. 3, 09.2010, p. 236-244.

Research output: Contribution to journalArticle

Murakami, T, Fukunaga, T, Takeshita, N, Hiratsuka, K, Abiko, Y, Yamashiro, T & Takano-Yamamoto, T 2010, 'Expression of Ten-m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice', Journal of Anatomy, vol. 217, no. 3, pp. 236-244. https://doi.org/10.1111/j.1469-7580.2010.01267.x
Murakami, Takashi ; Fukunaga, Tomohiro ; Takeshita, Nobuo ; Hiratsuka, Koichi ; Abiko, Yoshimitsu ; Yamashiro, Takashi ; Takano-Yamamoto, Teruko. / Expression of Ten-m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice. In: Journal of Anatomy. 2010 ; Vol. 217, No. 3. pp. 236-244.
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