Expression of stress response genes in barley Hordeum vulgare in a spaceflight environment

E. I. Shagimardanova, O. A. Gusev, V. N. Sychev, M. A. Levinskikh, M. R. Sharipova, O. N. Il'inskaya, G. Bingham, Manabu Sugimoto

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The transcriptome of barley Hordeum vulgare grown aboard the International Space Station was studied using microarray analysis. In the spaceflight environment, mRNA levels of over 500 genes were changed more than twofold; among them, genes of stress response proteins, in particular, heat shock proteins, pathogenesis-related proteins, and antioxidant proteins. Further analysis by real-time PCR confirmed enhanced transcription of reactive oxygen species scavenging genes. The superoxide dismutase (sod) mRNA level in the space environment was 6-fold higher than in earth conditions. The transcript levels of glutamyl transferase (gst), catalase (cat), and ascorbate peroxidase (apx) were increased in spaceflight 24, 18, and 3 times in comparison to ground control, respectively. For the first time, it has been shown that spaceflight environment can induce oxidative stress in plants.

Original languageEnglish
Pages (from-to)734-740
Number of pages7
JournalMolecular Biology
Volume44
Issue number5
DOIs
Publication statusPublished - 2010

Fingerprint

Space Flight
Hordeum
Heat-Shock Proteins
Ascorbate Peroxidases
Genes
Messenger RNA
Microarray Analysis
Transferases
Transcriptome
Catalase
Superoxide Dismutase
Real-Time Polymerase Chain Reaction
Reactive Oxygen Species
Proteins
Oxidative Stress
Antioxidants

Keywords

  • antioxidant defense
  • gene expression
  • Hordeum vulgare
  • microgravity
  • real-time PCR

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology

Cite this

Shagimardanova, E. I., Gusev, O. A., Sychev, V. N., Levinskikh, M. A., Sharipova, M. R., Il'inskaya, O. N., ... Sugimoto, M. (2010). Expression of stress response genes in barley Hordeum vulgare in a spaceflight environment. Molecular Biology, 44(5), 734-740. https://doi.org/10.1134/S0026893310050080

Expression of stress response genes in barley Hordeum vulgare in a spaceflight environment. / Shagimardanova, E. I.; Gusev, O. A.; Sychev, V. N.; Levinskikh, M. A.; Sharipova, M. R.; Il'inskaya, O. N.; Bingham, G.; Sugimoto, Manabu.

In: Molecular Biology, Vol. 44, No. 5, 2010, p. 734-740.

Research output: Contribution to journalArticle

Shagimardanova, EI, Gusev, OA, Sychev, VN, Levinskikh, MA, Sharipova, MR, Il'inskaya, ON, Bingham, G & Sugimoto, M 2010, 'Expression of stress response genes in barley Hordeum vulgare in a spaceflight environment', Molecular Biology, vol. 44, no. 5, pp. 734-740. https://doi.org/10.1134/S0026893310050080
Shagimardanova EI, Gusev OA, Sychev VN, Levinskikh MA, Sharipova MR, Il'inskaya ON et al. Expression of stress response genes in barley Hordeum vulgare in a spaceflight environment. Molecular Biology. 2010;44(5):734-740. https://doi.org/10.1134/S0026893310050080
Shagimardanova, E. I. ; Gusev, O. A. ; Sychev, V. N. ; Levinskikh, M. A. ; Sharipova, M. R. ; Il'inskaya, O. N. ; Bingham, G. ; Sugimoto, Manabu. / Expression of stress response genes in barley Hordeum vulgare in a spaceflight environment. In: Molecular Biology. 2010 ; Vol. 44, No. 5. pp. 734-740.
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