Expression of salivary miR-203a-3p was related with oral health-related quality of life in healthy volunteers

Terumasa Kobayashi, Takaaki Tomofuji, Tatsuya Machida, Toshiki Yoneda, Daisuke Ekuni, Tetsuji Azuma, Takayuki Maruyama, Akiko Hirose, Manabu Morita

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Oral health-related quality of life (OHRQoL) is a multidimensional construct that involves subjective evaluation of an individual’s oral health. Although it is difficult to evaluate OHRQoL biologically, recently, it has been reported that circulating microRNAs (miRNAs) in several body fluids could reflect various health conditions. The aim of this pilot study was to investigate whether salivary miRNAs expression differs according to OHRQoL in healthy volunteers. Forty-six volunteers (median age, 23.0 years) were recruited, and their OHRQoL was assessed using the Japanese version of the Oral Health Impact Profile (OHIP-J). Then, we compared salivary microRNA profiles of the high-OHRQoL group (≤25th percentile score of OHIP-J) and the low-OHRQoL group (≥75th percentile score of OHIP-J) using the polymerase chain reaction (PCR) array and the quantitative real-time PCR. There were no significant differences between the two groups in terms of oral health status. In the PCR array, miR-203a-3p and miR-30b-5p were significantly more expressed in the low-OHRQoL group (p < 0.05). Quantitative real-time PCR assay also showed that miR-203a-3p was more highly expressed in the low-OHRQoL group than in the high-OHRQoL group (p < 0.05). These observations suggest that expression of salivary miR-203a-3p was related with OHRQoL in healthy volunteers.

Original languageEnglish
Article number1263
JournalInternational Journal of Molecular Sciences
Volume18
Issue number6
DOIs
Publication statusPublished - Jun 13 2017

Fingerprint

Oral Health
health
Healthy Volunteers
Quality of Life
Health
polymerase chain reaction
Polymerase chain reaction
MicroRNAs
Real-Time Polymerase Chain Reaction
Polymerase Chain Reaction
body fluids
Body Fluids
Body fluids
profiles
Health Status
Volunteers
Assays

Keywords

  • Biomarkers
  • MicroRNA
  • Oral health-related quality of life
  • Saliva

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Spectroscopy
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Expression of salivary miR-203a-3p was related with oral health-related quality of life in healthy volunteers. / Kobayashi, Terumasa; Tomofuji, Takaaki; Machida, Tatsuya; Yoneda, Toshiki; Ekuni, Daisuke; Azuma, Tetsuji; Maruyama, Takayuki; Hirose, Akiko; Morita, Manabu.

In: International Journal of Molecular Sciences, Vol. 18, No. 6, 1263, 13.06.2017.

Research output: Contribution to journalArticle

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