Expression of root HvPIPs of barley seedling under chilling stress

Rui Jun Duan, Hui Yan Xiong, Maki Katsuhara

Research output: Contribution to journalArticle

Abstract

The plant plasma membrane aquaporin (plasma membrane intrinsic proteins, PIPs) is a kind of membrane intrinsic proteins which has high selectivity and water transport. They play important roles in many physiological activities in plant growth and development. In this study, seedlings of barley variety 'Haruna-nijo' were treated with chilling stress. Expression of water channel protein PIPs of root in two processes which were stress period (4℃, 48 h) and recovery period (16℃, 48 h) were analyzed with quantitative real-time reverse transcriptase PCR (qRT-PCR). And root hydraulic conductivity (Lpr), root length and seedling height were analyzed at the same time. The results showed that: after 4℃ and 48 h stress, the growth of the barley seedlings was significantly inhibited, but root growth was no significant changed; after 16℃, 48 h recovery, shoot height of stressed-seedling caught up that of control seedlings and root growth did not change significantly; root hydraulic conductivity decreased in the period of chilling stress and increased rapidly in recovery period but there were no significant difference. The results of qRT-PCR showed: the highest expression were HvPIP1;2 and HvPIP1;3, the lowest expression were HvPIP1;1 and HvPIP2;3. Compared with the control group, HvPIPs expression were decreased in all after cold treatment, in which HvPIP1;2, HvPIP1;3, HvPIP1;4, HvPIP1;5, HvPIP2;1, HvPIP2;2 were down-regulated significantly. In recovery period, most of HvPIPs expression were increased, espeacally HvPIP1;1, HvPIP1;2, HvPIP1;5, HvPIP2;3 were up-regulated significantly, but HvPIP1;4, HvPIP2;5 were down-regulated. This study found that HvPIPs expressions of barley root were down-regulated overall in chilling stress and in recovery growth most HvPIPs were up-regulated. Combined with changes of root hydraulic conductivity and plant growth, the role of barley HvPIPs in chilling resistance reaction speculated that HvPIPs effected water absorption were different in different stages.

Original languageEnglish
Pages (from-to)1203-1208
Number of pages6
JournalZhiwu Shengli Xuebao/Plant Physiology Journal
Volume50
Issue number8
DOIs
Publication statusPublished - Aug 1 2014

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Hordeum
Seedlings
root hydraulic conductivity
barley
seedlings
membrane proteins
Growth
Aquaporins
Membrane Proteins
plasma membrane
Cell Membrane
Reverse Transcriptase Polymerase Chain Reaction
blood proteins
Blood Proteins
Real-Time Polymerase Chain Reaction
root growth
reverse transcriptase polymerase chain reaction
plant growth
Plant Development
aquaporins

Keywords

  • Aquaporins
  • Barley
  • Chilling stress
  • Gene expression

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Expression of root HvPIPs of barley seedling under chilling stress. / Duan, Rui Jun; Xiong, Hui Yan; Katsuhara, Maki.

In: Zhiwu Shengli Xuebao/Plant Physiology Journal, Vol. 50, No. 8, 01.08.2014, p. 1203-1208.

Research output: Contribution to journalArticle

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abstract = "The plant plasma membrane aquaporin (plasma membrane intrinsic proteins, PIPs) is a kind of membrane intrinsic proteins which has high selectivity and water transport. They play important roles in many physiological activities in plant growth and development. In this study, seedlings of barley variety 'Haruna-nijo' were treated with chilling stress. Expression of water channel protein PIPs of root in two processes which were stress period (4℃, 48 h) and recovery period (16℃, 48 h) were analyzed with quantitative real-time reverse transcriptase PCR (qRT-PCR). And root hydraulic conductivity (Lpr), root length and seedling height were analyzed at the same time. The results showed that: after 4℃ and 48 h stress, the growth of the barley seedlings was significantly inhibited, but root growth was no significant changed; after 16℃, 48 h recovery, shoot height of stressed-seedling caught up that of control seedlings and root growth did not change significantly; root hydraulic conductivity decreased in the period of chilling stress and increased rapidly in recovery period but there were no significant difference. The results of qRT-PCR showed: the highest expression were HvPIP1;2 and HvPIP1;3, the lowest expression were HvPIP1;1 and HvPIP2;3. Compared with the control group, HvPIPs expression were decreased in all after cold treatment, in which HvPIP1;2, HvPIP1;3, HvPIP1;4, HvPIP1;5, HvPIP2;1, HvPIP2;2 were down-regulated significantly. In recovery period, most of HvPIPs expression were increased, espeacally HvPIP1;1, HvPIP1;2, HvPIP1;5, HvPIP2;3 were up-regulated significantly, but HvPIP1;4, HvPIP2;5 were down-regulated. This study found that HvPIPs expressions of barley root were down-regulated overall in chilling stress and in recovery growth most HvPIPs were up-regulated. Combined with changes of root hydraulic conductivity and plant growth, the role of barley HvPIPs in chilling resistance reaction speculated that HvPIPs effected water absorption were different in different stages.",
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