TY - JOUR
T1 - Expression of P45017α hydroxylase and P450 aromatase genes in the chicken gonad before and after sexual differentiation
AU - Yoshida, Kumiko
AU - Shimada, Kiyoshi
AU - Saito, Noboru
N1 - Funding Information:
1This research was supported by grants-in-aid from the Ministry of Education of Japan (04454106 and 05660326) and Monbusho International Scientific Research Program (Joint Research, 07044190) to K.S.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1996/5
Y1 - 1996/5
N2 - The onset and localization of P45017α-hydroxylase (P45017α) and P450 aromatase (P450arom) mRNA expression were studied in the gonads of chicken embryos at Days 4-9 of incubation by in situ hybridization analysis, and expression of both mRNAs was measured in the ovary and testis of the chicken after sexual differentiation by Northern and slot-blot analysis and was related to changes in concentration of 17β-estradiol (E2) in the gonad. In situ hybridization analysis showed the first detection of P45017α mRNA at Days 5-6 of incubation in the genetic male and female gonads and the first detection of P450arom at Day 6.5 of incubation in the female gonad but none was detected in the male gonad. Both mRNAs were observed in the medullary cords of the gonad. Slot-blot analysis demonstrated that levels of P45017α mRNA in the ovary remained high during the period between Day 10 of incubation and 7 days of posthatching, while those in the testis remained low at Days 10-14 of incubation but increased at Day 16 of incubation up to posthatching days. Levels of P450arom mRNA in the left ovary were invariably higher than those of the right ovary and testis throughout the period between Day 10 of incubation and 7 days of posthatching. These results suggest that expression of P450arom mRNA occurring in the genetic female gonad around Day 6 is associated, at least in a part, with sexual differentiation toward femaleness by increasing E2 production which may promote cellular proliferation of the left gonad. In contrast, in the genetic male gonad marked expression of P45017α mRNA with little expression of P450arom mRNA may lead to production of androgen which promotes the gonadal development to the testis.
AB - The onset and localization of P45017α-hydroxylase (P45017α) and P450 aromatase (P450arom) mRNA expression were studied in the gonads of chicken embryos at Days 4-9 of incubation by in situ hybridization analysis, and expression of both mRNAs was measured in the ovary and testis of the chicken after sexual differentiation by Northern and slot-blot analysis and was related to changes in concentration of 17β-estradiol (E2) in the gonad. In situ hybridization analysis showed the first detection of P45017α mRNA at Days 5-6 of incubation in the genetic male and female gonads and the first detection of P450arom at Day 6.5 of incubation in the female gonad but none was detected in the male gonad. Both mRNAs were observed in the medullary cords of the gonad. Slot-blot analysis demonstrated that levels of P45017α mRNA in the ovary remained high during the period between Day 10 of incubation and 7 days of posthatching, while those in the testis remained low at Days 10-14 of incubation but increased at Day 16 of incubation up to posthatching days. Levels of P450arom mRNA in the left ovary were invariably higher than those of the right ovary and testis throughout the period between Day 10 of incubation and 7 days of posthatching. These results suggest that expression of P450arom mRNA occurring in the genetic female gonad around Day 6 is associated, at least in a part, with sexual differentiation toward femaleness by increasing E2 production which may promote cellular proliferation of the left gonad. In contrast, in the genetic male gonad marked expression of P45017α mRNA with little expression of P450arom mRNA may lead to production of androgen which promotes the gonadal development to the testis.
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U2 - 10.1006/gcen.1996.0064
DO - 10.1006/gcen.1996.0064
M3 - Article
C2 - 8998967
AN - SCOPUS:0029981877
VL - 102
SP - 233
EP - 240
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
SN - 0016-6480
IS - 2
ER -